Camera Calibration Technical Report:
Video Camera Calibration for Optical Densitometry

Video Camera Calibration for Optical Densitometry

Richard A Baldock
Ian Poole
MRC Human Genetics Unit, Crewe Road, Edinburgh, EH4 2XU, UK.

Abstract:

We describe an efficient technique by which to calibrate video cameras to record optical density (OD) from microscopic images. The method corrects for variation over the field of the bright and dark field intensities, does not assume a linear response of the camera to the incident intensity and requires a single calibration filter.

Key words optical density, camera calibration, shading correction, microscopic images.