EMAGE mouse gene expression spatial database

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EMAGE:10166

2410004A20Rik RIKEN cDNA 2410004A20 gene ( MGI:1914241)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000131. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000131_01	euxassay_000131_02	euxassay_000131_03	euxassay_000131_04

euxassay_000131_05	euxassay_000131_06	euxassay_000131_07	euxassay_000131_08	euxassay_000131_09

euxassay_000131_10	euxassay_000131_11	euxassay_000131_12	euxassay_000131_13	euxassay_000131_14

euxassay_000131_15	euxassay_000131_16	euxassay_000131_17	euxassay_000131_18	euxassay_000131_19

euxassay_000131_20	euxassay_000131_21	euxassay_000131_22	euxassay_000131_23	euxassay_000131_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10166_wholemount_strong.wlz

10166_wholemount_moderate.wlz

10166_wholemount_weak.wlz

10166_wholemount_possible.wlz

10166_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10166_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

homogeneous

moderate expression: see section 05

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 07

trigeminal v ganglion

strong

homogeneous

strong expression: see section 05 06 07 moderate expression: see section 08

vagus x ganglion

strong

homogeneous

strong expression: see section 06 07

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 06

dorsal root ganglion

moderate

homogeneous

moderate expression: see section 08 09 10 11 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1522
Entity Detected:	2410004A20Rik, RIKEN cDNA 2410004A20 gene ( MGI:1914241)
Sequence:	sense strand is shown >T1522 CGAGGCTGCCACCCGGCCGGCTCCCGGGAAGGTCCGCAAGGCGGCCACCCAGCCGGCTCCGGTGCAGGTT TGCCAGGAGGCCACCCAGTTGGCTCCCGTGAAGGTCCGCGAGGCGGCCACCCAGCCGGCTTCCGGGAAGG TCCGCGAGGCGGCCACCCAGTTGGCTCCTGTGAAGGTCCGCAAGGCAGCCACCCAGTTGGCTCCTGTGAA GGTCCACGAGGCGGCCACCCAGCCGGCTCCGGGGAAGGTCAGCGATGCTGCCACGCAGTCGGCTTCGGTG CAGGTTCGTGAGGCTGCCACGCAGCTGTCTCCCGTGGAGGCCACTGATACTAGCCAGTTGGCTCAGGTGA AGGCTGATGAAGCCTTTGCCCAGCACACTTCAGGGGAGGCCCACCAGGTTGCCAATGGGCAGTCTCCCAT TGAAGTCTGTGAGACTGCCACCGGGCAGCATTCTCTAGATGTCTCTAGGGCCTTGTCCCAGAAGTGTCCT GAGGTTTTTG
Notes:	The probe template was PCR amplified from IMAGE:805697 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:805697 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10167 same embryo
	EMAGE:10165 same embryo
	EurExpress:euxassay_000131 same experiment
	MGI:4822672 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source