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EMAGE:10208

Zfp646 zinc finger protein 646 ( MGI:3665412)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:10208 EMAGE:10208 EMAGE:10208 EMAGE:10208 EMAGE:10208
"Pseudo-wholemount" of euxassay_008221. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008221_01 euxassay_008221_02 euxassay_008221_03 euxassay_008221_04
EMAGE:10208 EMAGE:10208 EMAGE:10208 EMAGE:10208 EMAGE:10208
euxassay_008221_05 euxassay_008221_06 euxassay_008221_07 euxassay_008221_08 euxassay_008221_09
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euxassay_008221_10 euxassay_008221_11 euxassay_008221_12 euxassay_008221_13 euxassay_008221_14
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euxassay_008221_15 euxassay_008221_16 euxassay_008221_17 euxassay_008221_18 euxassay_008221_19
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euxassay_008221_20 euxassay_008221_21 euxassay_008221_22 euxassay_008221_23 euxassay_008221_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:10208Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
10208_wholemount_strong.wlz
10208_wholemount_moderate.wlz
10208_wholemount_weak.wlz
10208_wholemount_possible.wlz
10208_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:10208_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1695
Entity Detected:Zfp646, zinc finger protein 646 ( MGI:3665412)
Sequence:sense strand is shown

>T1695
TGGCCTCGAGCCAGATTCGGACGAGGGCACCAGCAGCAAGCAGAGAGGATTCCTCTACAGCCTCGCTTCC
GAACCCCTCACCCCAGTGGCCTGCAGACCTCAGTCTCTCCCTCTGAATGACAGGTCTCCAAAGAACAGTT
GACTGAAGGATGGGGCAGGCAGGGAGAGGCTTGCTCTCCTCATTTTGCTCAGGAGTTGCTTGGGCATCCC
CATCTCCTCTTCCCACGAAGAGGACCCTAATCTGGCTTTTTCCCCCAAGAAGGGCATGAGGTAGTCTTCA
TGTCTGTGCCCATGAAGGATGAAGGATCTCCTTCCCAACCCTTTGTCACCATGATTTTCCAGGGCCAGAC
TCTGCCAAAATGCAGAGAGAGCCAGATTCTCACCCAGGGAGAGGCAGATCAGAGAGAACCACTGGTGCGA
AAGCTGCCTGTGACAGGGGAGCCTTTTACTATAGTTTGTAACTTATTTTCTAAAATCTATTTTGTAACAA
TTTAAGTTTTAAAAAAGGAAATTGCTCCCCAAACCCCCCACCCCCAAGGAAAAAAAAAACAACTTTTTTT
TTCACAACA
Notes:The probe template was PCR amplified from IMAGE:476615 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:476615 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:10209 same assay
 EurExpress:euxassay_008221 same experiment
 EMAGE:10207 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS