EMAGE mouse gene expression spatial database

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EMAGE:10448

Ces2e carboxylesterase 2E ( MGI:2443170)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000577. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000577_01	euxassay_000577_02	euxassay_000577_03	euxassay_000577_04

euxassay_000577_05	euxassay_000577_06	euxassay_000577_07	euxassay_000577_08	euxassay_000577_09

euxassay_000577_10	euxassay_000577_11	euxassay_000577_12	euxassay_000577_13	euxassay_000577_14

euxassay_000577_15	euxassay_000577_16	euxassay_000577_17	euxassay_000577_18	euxassay_000577_19

euxassay_000577_20	euxassay_000577_21	euxassay_000577_22	euxassay_000577_23	euxassay_000577_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10448_wholemount_strong.wlz

10448_wholemount_moderate.wlz

10448_wholemount_weak.wlz

10448_wholemount_possible.wlz

10448_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10448_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T800
Entity Detected:	Ces2e, carboxylesterase 2E ( MGI:2443170)
Sequence:	sense strand is shown >T800 CTCAGNCTGTTGGCCTACTGGATAGCACACAGATATCCTGGAGCAACACAGACTCAGATCTAAGAGCTGA CTATGCCACTATACAAACTTCTTGGATGGCTGAATGCTGTGGCCTGTGGGGTCCTGCTTCTCGTTCTGCA TGTGCAAGGTCAGGACTCAGCCAGCCCCATCAGGAACACACACACAGGCCAGGTGAGAGGCAGCCTTGTC CACGTGAAGGACACTGACATCGCTGTTCATACCTTCCTGGGAATCCCCTTTGCCAAGCCTCCTGTAGGAC CACTGCGCTTTGCACCTCCTGAGGCCCCTGAGCCATGGAGTGGGGTGAGGGATGGGACCTCACATCCAAA CATGTGTCTACAAAATGACAATTTGATGGGTTCAGAGGATCTGAAGATGATGAACTTGATCCTGCCTCCC ATCTCTATGTCTGAGGACTGCCTGTATCTCAACATCTATGTACCAGCCCATGCCCATGAGGGTTCTAACC TACCTGTGATGGTATGGATCCATGGTGGTGCACTCACTGTTGGCATGGCTTCCATGTATGATGGATCCAT GCTG
Notes:	The probe template was PCR amplified from IMAGE:1921057 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1921057 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10450 same embryo
	EMAGE:10449 same embryo
	EMAGE:10451 same embryo
	EurExpress:euxassay_000577 same experiment
	MGI:4823823 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source