EMAGE mouse gene expression spatial database

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EMAGE:10453

Tspan17 tetraspanin 17 ( MGI:1921507)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000923. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000923_01	euxassay_000923_02	euxassay_000923_03	euxassay_000923_04

euxassay_000923_05	euxassay_000923_06	euxassay_000923_07	euxassay_000923_08	euxassay_000923_09

euxassay_000923_10	euxassay_000923_11	euxassay_000923_12	euxassay_000923_13	euxassay_000923_14

euxassay_000923_15	euxassay_000923_16	euxassay_000923_17	euxassay_000923_18	euxassay_000923_19

euxassay_000923_20	euxassay_000923_21	euxassay_000923_22	euxassay_000923_23	euxassay_000923_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10453_wholemount_strong.wlz

10453_wholemount_moderate.wlz

10453_wholemount_weak.wlz

10453_wholemount_possible.wlz

10453_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10453_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

homogeneous

moderate expression: see section 11 12 13 14 15 16

hindbrain

weak

homogeneous

weak expression: see section 13 14 15 not examined expression: see section 07 08 09 10 11 12 16 17 18

medulla oblongata basal plate

strong

homogeneous

strong expression: see section 07 08 09 10 15 16 17 18 not examined expression: see section 12

pons mantle layer

strong

homogeneous

strong expression: see section 07 moderate expression: see section 18 weak expression: see section 08 09

midbrain floor plate

strong

homogeneous

strong expression: see section 10 11 15

facial vii ganglion

strong

homogeneous

strong expression: see section 06 07 20 21 22 moderate expression: see section 04 05

inferior glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 07 18 19 moderate expression: see section 06

superior glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 06 moderate expression: see section 07 weak expression: see section 18

trigeminal v ganglion

strong

homogeneous

strong expression: see section 06 07 08 09 18 19 20 21 22 23 moderate expression: see section 03 04 05

vagus x ganglion

strong

homogeneous

strong expression: see section 08 moderate expression: see section 09 17 not examined expression: see section 06 07 18 19

vestibulocochlear viii ganglion vestibular component

moderate

homogeneous

moderate expression: see section 04

dorsal root ganglion

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2252
Entity Detected:	Tspan17, tetraspanin 17 ( MGI:1921507)
Sequence:	sense strand is shown >T2252 TGGCCTCGAGCCAGATTCGGATCCTTGGTGAGAAGGGCGTTCTCTCCAACATCTCGGCGCTGACAGATCT GGGCGGTCTTGACCCCGTGTGGCTGTTTGTGGTGGTGGGGGGAGTCATGTCAGTGTTGGGCTTCGCCGGC TGCATTGGGGCCCTCCGGGAAAACACCTTCCTGCTCAAGTTCTTCTCTGTGTTCCTCGGCCTCATCTTCT TCCTGGAGCTGGCCGCGGGGATCCTGGCCTTCGTCTTCAAGGATTGGATCCGAGACCAACTTAATCTCTT CATCAACAACAATGTCAAAGCCTACCGGGATGATCTCGACCTTCAGAACCTTATCGACTTTGCTCAGGAA TACTGGTCTTGCTGTGGTGCCCGAGGGCCCAATGACTGGAACCTCAATATCTACTTCAACTGCACTGACT TGAACCCAAGCCGTGAGCGCTGTGGGGTGCCCTTTTCCTGCTGTGTTAGGGACCCAGCGGAAGATGTCCT CAATACCCAGTGTGGCTATGACATCCGACTCAAACTGG
Notes:	The probe template was PCR amplified from IMAGE:1064383 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1064383 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10455 same embryo
	EMAGE:10454 same embryo
	EMAGE:10452 same embryo
	EurExpress:euxassay_000923 same experiment
	MGI:4828962 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source