EMAGE mouse gene expression spatial database

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EMAGE:10616

Fam164a family with sequence similarity 164, member A ( MGI:1914556)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000836. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000836_01	euxassay_000836_02	euxassay_000836_03	euxassay_000836_04

euxassay_000836_05	euxassay_000836_06	euxassay_000836_07	euxassay_000836_08	euxassay_000836_09

euxassay_000836_10	euxassay_000836_11	euxassay_000836_12	euxassay_000836_13	euxassay_000836_14

euxassay_000836_15	euxassay_000836_16	euxassay_000836_17	euxassay_000836_18	euxassay_000836_19

euxassay_000836_20	euxassay_000836_21	euxassay_000836_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10616_wholemount_strong.wlz

10616_wholemount_moderate.wlz

10616_wholemount_weak.wlz

10616_wholemount_possible.wlz

10616_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10616_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

telencephalon

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

hindbrain

moderate

homogeneous

moderate expression: see section 06 07 08 11 12 13 14 15 16 17 18 not examined expression: see section 05

medulla oblongata

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

metencephalon

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

midbrain

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

facial vii ganglion

strong

homogeneous

strong expression: see section 05 06 08 20 21 22

inferior glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 07 08 18 20

superior glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 08 18

trigeminal v ganglion

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 17 18 20 21 22

vagus x ganglion

strong

homogeneous

strong expression: see section 09 10 11 17

vestibulocochlear viii ganglion cochlear component

strong

homogeneous

strong expression: see section 07 08 09 18 20

vestibulocochlear viii ganglion vestibular component

strong

homogeneous

strong expression: see section 06 07 08 09 18 20 21

spinal cord

moderate

homogeneous

moderate expression: see section 11 12 13 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 14 15 17 18 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 15 16 17 18 19

not examined

homogeneous

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1003
Entity Detected:	Fam164a, family with sequence similarity 164, member A ( MGI:1914556)
Sequence:	sense strand is shown >T1003 TCCTCGAGNCTGTTGGCCTACTGGAGGCGGCGCGTCATGGACGGACTGGAAGAAAATGGAAGTGTTGTGC AAGTTGGAGACTTGTTGCCCTGCAAGATTTGCGGAAGGACCTTCTTCCCGTTGGCTCTGAAAAAACATGG ACCTATTTGCCAGAAAACTGCCACTAAGAAGCGGAAGACTTTTGATTCCAGCCGGCAGAGAGCTGAAGGG ACTGATATTCCCACGGTCAAACCTCTCAAACCCAGGCCAGAACCACCAAAGAAGCCATCCAATTGGAGAC GAAAGCACGAGGAGTTCATTGCCACCATCAGAGCAGCGAAAGGCCTGGACCAGGCTCTCAAGGAGGGCGG CAAGCTGCCTCCTCCTCCTCCGCCCTCTTACGACCCAGATTACATCCAGTGTCCGTATTGCCAGAGGAGA TTCAATGAAAATGCAGCCGACAGACATATAAACTTTTGTAAAGAACAGGCAGCACGTATTAGTAATAAGG GCAAATTTTCTACTGATTCTAAAGGAAAGCCAGCTTCTCGGCCACAGTATAAGCCGTCTCCGCTTAAA
Notes:	The probe template was PCR amplified from IMAGE:2064672 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2064672 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10617 same embryo
	EMAGE:10615 same embryo
	EMAGE:10614 same embryo
	EurExpress:euxassay_000836 same experiment
	MGI:4824717 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source