EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10653

Slc25a39 solute carrier family 25, member 39 ( MGI:1196386)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019719. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019719_01	euxassay_019719_02	euxassay_019719_03	euxassay_019719_04

euxassay_019719_05	euxassay_019719_06	euxassay_019719_07	euxassay_019719_08	euxassay_019719_09

euxassay_019719_10	euxassay_019719_11	euxassay_019719_12	euxassay_019719_13	euxassay_019719_14

euxassay_019719_15	euxassay_019719_16	euxassay_019719_17	euxassay_019719_18	euxassay_019719_19

euxassay_019719_20	euxassay_019719_21	euxassay_019719_22	euxassay_019719_23	euxassay_019719_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10653_wholemount_strong.wlz

10653_wholemount_moderate.wlz

10653_wholemount_weak.wlz

10653_wholemount_possible.wlz

10653_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10653_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

weak

regional

weak expression: see section 04 05 06 07 19 20 21 22 23

adrenal gland

moderate

regional

moderate expression: see section 10 11 19 weak expression: see section 12 20 21

submandibular gland primordium

moderate

regional

moderate expression: see section 09 19 20 weak expression: see section 08 10 11 21 22

pancreas

weak

regional

weak expression: see section 09 10 11 12

midgut

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 weak expression: see section 08 09 10 11 12

lower jaw incisor

moderate

regional

moderate expression: see section 13 17 18 19 weak expression: see section 14

lower jaw molar

moderate

regional

moderate expression: see section 09 20

upper jaw incisor

moderate

regional

moderate expression: see section 13 18 19 weak expression: see section 14

upper jaw molar

moderate

regional

moderate expression: see section 09 20

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 12

testis

weak

regional

weak expression: see section 07 08 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T56042
Entity Detected:	Slc25a39, solute carrier family 25, member 39 ( MGI:1196386)
Sequence:	sense strand is shown >T56042 TGCCTTTGTGAAGATTGTGCGGCATGAGGGCACTAGGACCCTGTGGAGCGGCCTCCCAGCCACCCTGGTG ATGACCGTGCCAGCTACTGCTATCTACTTCACTGCTTACGACCAACTCAAGGCCTTCCTGTGTGGTCAGT CCTTGACCTCTGACCTCTACGCACCCATGGTGGCTGGTGCCCTCGCCCGAATGGGCACCGTGACAGTTGT CAGCCCCTTGGAGCTCGTGCGGACCAAGCTGCAGGCTCAGCATGTGTCATACCGTGAGCTGGCTTCCTCT GTTCAAGCTGCGGTGACTCAGGGTGGCTGGCGCTCTCTGTGGCTGGGCTGGGGTCCCACAGCTCTTCGAG ATGTCCCCTTCTCAGCTCTGTACTGGTTCAACTACGAGTTGGTGAAGAGCTGGCTGAGCGGACTGAGACC AAAAGACCAGACATCGGTGGGCATCAGCTTCGTGGCCGGTGGCATCTCAGGAATGGTGGCTGCCACCCTT ACCCTACCCTTCGATGTGGTGAAGACACAGCGACAGATGTCACTGGGAGCGGTGGAGGCTGTGAGAGTGA AGCCGCCCAGAGTGGACTCCACCTGGCTCCTGCTTCGCAGAATCCGGGCTGAATCTGGCACCAGGGGACT CTTTGCAGGCTTCCTCCCCAGGATCATCAAGGCTGCACCCTCCTGTGCCATCATGATCAGCACTTACGAG TTTGGCAAAAGCTTCTTCCAGAGGCTCAACCAGGAGCAGCCTCTGGGCCGCTGAAAGTCGGGGAGCAACC GGCTCTTGCACGGGTGGGATCAGGAGACTGAGCCAAGTGCCTTCCCTCCCAGTCCAGTCACCCAGGCTGT CCTCAGACCTGGATTCCTGCTGTTTCCCTTCCCAGGATT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TGCCTTTGTGAAGATTGTGC; Reverse Primer - name:unspecified, sequence:AATCCTGGGAAGGGAAACAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4828160 same experiment
	EurExpress:euxassay_019719 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source