EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:10738

1700011H14Rik RIKEN cDNA 1700011H14 gene ( MGI:1914332)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001019. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001019_01	euxassay_001019_02	euxassay_001019_03	euxassay_001019_04

euxassay_001019_05	euxassay_001019_06	euxassay_001019_07	euxassay_001019_08	euxassay_001019_09

euxassay_001019_10	euxassay_001019_11	euxassay_001019_12	euxassay_001019_13	euxassay_001019_14

euxassay_001019_15	euxassay_001019_16	euxassay_001019_17	euxassay_001019_18	euxassay_001019_19

euxassay_001019_20	euxassay_001019_21	euxassay_001019_22	euxassay_001019_23	euxassay_001019_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10738_wholemount_strong.wlz

10738_wholemount_moderate.wlz

10738_wholemount_weak.wlz

10738_wholemount_possible.wlz

10738_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10738_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

stomach

moderate

regional

moderate expression: see section 02 03 04 05

midgut

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 11 12 13 14

kidney calyx

moderate

regional

moderate expression: see section 07 08 11 19 20 21

kidney pelvis

moderate

regional

moderate expression: see section 09 10 17 18

testis

moderate

regional

moderate expression: see section 07 08 09 10 11 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1099
Entity Detected:	1700011H14Rik, RIKEN cDNA 1700011H14 gene ( MGI:1914332)
Sequence:	sense strand is shown >T1099 TCCTCGAGNCTGTTGGCCTACTGGACAGAACAGGGAAGATGTCATGAAGGAAACACCTGCTTTGGAACAT GGGCCTGGGCCACTCCAAGGCTCACCCTCGGGTGATCAAAGTCACACCTTTACAAAGCCAAGAAACAGAG ACACCCTCAACTGGCCCTGTGTTCTTTGCATTGAATCGGAACCTGGAAGAAGAAAGCTCATTCACGAGAC TGCAGGACCAGAATCGAACTCGAGAAGGACAGCTACCACCGCTTCGAGAAACTTGGTATGGAAGACTCCC GGCAGTATCCAGGGCCATGTATCTTGACATCCCACTGAAACATGAAGAAACAAGTATTATCAAAAGGCAT CCACCCCGAAGAATTCAAAAGCTTGAACCCATTGACCTGCCACAAGCAATTACCTCTGAAAGGCTTCTGT GCCATCAAGAAGGCAGAACAAAGTCCAACACAAAGCAGGAAAATGAGAAGAAAATTCAACTTCCTATGTA CACTTCTGGGAAAAGACAATATTTGCATAAGATGAAAATGCTAGAGATGAACCATAAAAGACAAGAGGCC CA
Notes:	The probe template was PCR amplified from IMAGE:2123348 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2123348 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10736 same embryo
	EMAGE:10735 same embryo
	EMAGE:10734 same embryo
	EMAGE:10737 same embryo
	EMAGE:10733 same embryo
	EurExpress:euxassay_001019 same experiment
	MGI:4822624 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source