EMAGE mouse gene expression spatial database

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EMAGE:10834

Ankrd24 ankyrin repeat domain 24 ( MGI:1890394)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001100. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001100_01	euxassay_001100_02	euxassay_001100_03	euxassay_001100_04

euxassay_001100_05	euxassay_001100_06	euxassay_001100_07	euxassay_001100_08	euxassay_001100_09

euxassay_001100_10	euxassay_001100_11	euxassay_001100_12	euxassay_001100_13	euxassay_001100_14

euxassay_001100_15	euxassay_001100_16	euxassay_001100_17	euxassay_001100_18	euxassay_001100_19

euxassay_001100_20	euxassay_001100_21	euxassay_001100_22	euxassay_001100_23	euxassay_001100_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10834_wholemount_strong.wlz

10834_wholemount_moderate.wlz

10834_wholemount_weak.wlz

10834_wholemount_possible.wlz

10834_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10834_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pituitary gland

weak

regional

weak expression: see section 11 12 13 14 15 16 17

vagus x ganglion

weak

regional

weak expression: see section 07 08 09 19 20

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 20 21 weak expression: see section 06 07 08 09 18 19

cochlea

moderate

regional

moderate expression: see section 02 04 05 22 23

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 13 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4337
Entity Detected:	Ankrd24, ankyrin repeat domain 24 ( MGI:1890394)
Sequence:	sense strand is shown >T4337 GAGCCTGGGCCGGGAGGTGGAGAGCCTGCAGAGCCGTCTGTCCCTGCTGGAGAATGAGAGAGAAAACACA AGCTATGATGTAGCTACCCTGCAGGATGAGGAGGGTGAGATGCCTGACTTCCCAGGAGCTGATGCACTGA TGCCAAAGAACCAGAGTCCATCAGCAGAGGAAATAGTTGCTTCCCTACAGGAGCAGGTGGCTCAGCTCAC CAGGCAGAACCAGGAGCTGCTGGAAAAGGTCCAGATCCTTGAGGAGTTTGAAAAGGATGAGGCGCAGATG GCAGAAGAAAGTCAGGCCGAGGTCGTCCCCCTGGTCTTGTATGAATCCTTACGGGCAGAGCTTGAGCAGC TTCGGAGGCAATACACAGAGGCCATGCATTCGCAGCAGCAGCAGCAGGAGGGGGAGCCACCCAGGGCTCA AGAAGGAGAAGAGACAGCATACCAGGAAATCAAGGATAAGGGAATCACTATCCAGAATGGACCCAGCGTC CCAGACCTCAACGGCACCACATATGCAGAAACCAAAGCAAATGGAATGGAACTCCAAGCAGGAGGCTCCA AGGGTGTCTGGAATACTGAAGCAGGGGTTTCAGAAGCAGCACCCATAGAACCCGAGGCTGCAGGTTCAGA GGCCACGGGGAAAGACAGACTGGCAGCCAAGGAAATGGACACTAGTGCTACTATGGCTGAGGCCCTAAAT GTGAAAGCTCTAGGTGACAATGCCGAAAGTGAGCCAGTGGCTGCCGAAGATACAGGAGGAAAAGAGAATC CAGGAATGAAAGCTGATGAGGTGGATGTGTTGGCACAGGCAGGGCTCACAGGTACAGTGATTAGAAACAT GGAGGCCATCGGAGTGCGGGATACAGGAATTCAGGCCACAGGATTAGAGGCTAAGGCAGTGAAGACCACT GGAGTGCAGGCCACAGTGGCGGAGGTCATAGGAGTGAAGGTCACAGGAGTGCAGACCACAGCGATAGAAG CTATAGGAGTCAAGGACACCACCCAGGTGGCCACAGGAGCGCAGGCCGATTGCTGGCAGGCCACGGAGGC AGACTGCACCGGAGCGCAGGACACAGCTATGGAGCCCACGGGGGCACAGGCCACTGTGACAGAGACTACG GAAGCCGAGACCAGCGGCACGGAGGACCCTTGCGCTGCGATCCTGCACCCGGGGGCGGCGGCGGCGGCGC TGCAGGCCGAGCTGGAGACCCGGATCCGCGGTCTGGAGGAGGCGCTACGCAGAAGGGAGCGGGAGGCTGC GGCCGAGTTAGAGGCTGCTCGGGGCCGTTTTGCAGAGGCTGAGGAGGCGGCGCGGGGGCGGAGCCGCGAG CTAGAGGCGCTTCGGGAATTGCTGGCCACGGCCACGGCTACCGGCGAGCGCGCACGCACAGAGGCCGCCG AGTTGCGCCAAGCGCTAGCTGCCTCCGAGGCTCGGGTCGCCGAACTCAGCTCTACCGTGGACGCCGCTCG CGAGGAGCTGGAGCGCATGCGCGGGGCCTCGGTTCCTGCGGACGAGCACGAGCACGCGCTGAGCGCCCTG CGCGACCACGTGACCCGGCTGCAGGCGCAGCTGGCGGACCTGGCGCGCAGGCACGAGAAGACTAGCGCAG AGGTCTTCCAGATCACAGACCTGTCCAAAGAAGTCTTTACACTGAAGGAGGCTCTGAAGGTGCAGCAGTC CACCCCAGCCAGCTCCAAGGAGGAGGAGGCTCTGCGCGGCCAGGTGACAGCCCTGCAGCAGCAGATACAG GAGGAGGCCCGGGAGCATGGCGCTGTGGTGGCTTTATACCGCACCCATCTCCTGTATGCCATTCAGGGAC AGATGGATGAGGATGTACAGTGCATTTTGAGCCAGATTCTGCAGATGCAGCGGCTTCAGGCTCAGGGTCG CTGAGGACACAGCTCAACTGTTCAGTCTCCATCCTTCACCTCTACAAGCAGAAGCCACAGACACCATCTT CCCCAGCTCCCAGGGGATGAACAAGATTCAACACTCAATGGGAGACCAGCCTGGGGCCTTATCGATGGTG GCCAGCTGACCACCCCACACTCCCTATAGTCTGTTCCTCTGATTCCCAGCACTCCCCATTTGATATATTG GGAACCCTGATATGCCACCTACATCACAACCCCCACTCAGGAGGACAAGCTTGGTGTGTGTATGTGTGTG TGTAAAAGGGCGGGGGGTGGGGTGGGGCAGGAATACCCAGTGTGACACACTGGGTAAGGGCAGCTATGGG GCAGGGGACTCAGGACACAGTTTGATCTTCTGGTTCCATAACACGACTATGGCAATAAATGTAAGTGCAA TGTAATATTTAAAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4526980 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4526980 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10835 same embryo
	EMAGE:10836 same embryo
	EMAGE:10837 same embryo
	EurExpress:euxassay_001100 same experiment
	MGI:4823132 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source