EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11183

U2af2 U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 ( MGI:98886)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003614. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003614_01	euxassay_003614_02	euxassay_003614_03	euxassay_003614_04

euxassay_003614_05	euxassay_003614_06	euxassay_003614_07	euxassay_003614_08	euxassay_003614_09

euxassay_003614_10	euxassay_003614_11	euxassay_003614_12	euxassay_003614_13	euxassay_003614_14

euxassay_003614_15	euxassay_003614_16	euxassay_003614_17	euxassay_003614_18	euxassay_003614_19

euxassay_003614_20	euxassay_003614_21	euxassay_003614_22	euxassay_003614_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11183_wholemount_strong.wlz

11183_wholemount_moderate.wlz

11183_wholemount_weak.wlz

11183_wholemount_possible.wlz

11183_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11183_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15

submandibular gland primordium

strong

regional

strong expression: see section 06 07 18 19 moderate expression: see section 08 09 16 17

thyroid gland

moderate

regional

moderate expression: see section 10 11 15 16

vibrissa

strong

regional

strong expression: see section 05 06 07 20 21 22

naris

weak

regional

weak expression: see section 13

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 09 10 11 12 14 15 16 17 18 19

nasal cavity respiratory epithelium

weak

regional

weak expression: see section 11 12 15 16

esophagus

strong

regional

strong expression: see section 11 12 13

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 weak expression: see section 07 08

hindgut

weak

regional

weak expression: see section 11 12 13 14

rectum

weak

regional

weak expression: see section 13 14

midgut

moderate

regional

moderate expression: see section 06 weak expression: see section 07 08 09 10 11 12 13 14 15 16

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23

bladder

weak

regional

weak expression: see section 13 14 15

urethra of male

weak

regional

weak expression: see section 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6579
Entity Detected:	U2af2, U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2 ( MGI:98886)
Sequence:	sense strand is shown >T6579 AGCTGCGGGTCAGATTCCAGCCACTGCCCTTCTCCCGACCATGACTCCTGATGGTCTGGCTGTGACCCCA ACGCCAGTGCCTGTGGTTGGGAGCCAGATGACCAGACAGGCCAGACGTCTCTACGTGGGCAACATCCCTT TTGGCATTACTGAGTGAAATATTGTGGGGCTGAGATCCCTCGTAGCAACAAAAAGTTTGCTACCATCGTT GAAGGCAAGTAAGGTCCATTCTGACTTTCTCAACCTGCACTTTGCTACATTTGATAAACCAGCCCCCGGA CTCCAGGGCCAGATCCCTCCACATCACTCTTTTCTCCTCCCCTCCCGCCCTACCCCTTCTTCCCCTGCTC CTCCACCCCTCCCCGCCCCTGCACTCTACTTGTTCTGCCTCTCTGGGCACCCTTCCTGTCCCTTTGCTTG TCTTTCTCTTCCTGCCTGTTGCTCTCCTACCCTTTCCTGCCTTGTTTGCAATGGGCATTTCTTCCTTGTG TCATCATGTTTTTTGTTTTTGTTTTTTTTTCCTCTTGCTGTCACATCCTTTCATCTTTTCTTGAATAACT TTTCCTAACTTTTTGGGCTCCATGGCTCATCCCTCATGCCTGTCCTCCTGTCTGGCCCATTTGCTCCCTG TCTCCTGTCCTTTGGTATTGCTTCTTCTCTGTTTTCTCATTTTCCTCCTGCATTTTTTCCTACTATTGAC CTCTACACCCCTGACCCATATTCCACTGTCCCTTTCCCTCCCCGTTCTCCCTTCCCCCATTCCTCTCCCC TTACCCCCAAACCCCTCTGTGTCTCCCTCTCTCACCCTTCCCCTCCTCTCTCCACCTCCCTCCCCGCCCT TCCCTGTCTCCTATTCCCTCTGCAGGAGGCTATGATGGACTTCTTCAACGCCCAGATGCGCTTGGGAGGG TTGACTCAGGCCCCTGGCAACCCAGTCTTGGCTGTGCAGATAAATCAAGACAAGAATTTTGCCTTTTTGG AGTTCCGGTCAGTGGATGAGACGACCCAGGCCATGGCATTTGATGGCATCATCTTCCAGGGCCAGTCATT GAAGATTCGAAGGCCTCATGACTATCAGCCATTGCCTGGCATGTCAGAGAACCCCTCTGTTTATGTGCCT GGAGTTGTATCCACGGTAGTCCCAGATTCTGCCCACAAGCTGTTCATCGGGGGTTTGCCCAATTACCTAA ATGATGACCAGGTAAAAGAGCTGCTGACATCCTTTGGGCCTCTCAAGGCCTTCAACTTGGTTAAGGATAG TGCCACAGGGCTCTCCAAGGGCTATGCCTTCTGTGAGTACGTGGACATCAACGTCACAGATCAGGCCATT GCGGGGCTGAATGGGATGCAGCTAGGGGACAAGAAGCTGCTTGTCCAGAGGGCGAGTGTGGGAGCCAAGA ATGCCACGCTGAGCACCATCAATCAGACACCTGTGACCCTTCAAGTGCCCGGCCTGATGAGCTCTCAGGT GCAGATGGGCGGTCACCCAACTGAGGTCCTGTGCCTCATGAACATGGTGCTGCCTGAGGAGCTGCTGGAC GATGAGGAGTATGAGGAGATTGTAGAGGACGTACGAGACGAGTGCAGCAAGTATGGGTTGGTCAAATCCA TTGAAATTCCCCGCCCCGTGGACGGCGTCGAGGTGCCTGGCTGTGGAAAGATCTTCGTGGAGTTCACCTC TGTGTTTGACTGCCAGAAAGCCATGCAGGGTCTAACCGGTCGCAAGTTCGCCAACAGAGTGGTGGTCACA AAATACTGTGACCCTGATTCTTACCACCGTCGGGACTTCTGGTAGAGGTGGCTGGGGGAGGGTGGGGGCA GGGCTGGCTGGGGACTTTCCCCCCACCTGTCCCCTCCTCCTTTCCCCCCTCTTGAAGAAGATGGGCAGAG GAGTGACAGCCGAATGACAGCCGGCAGCAACTGGAATGGCAGCAATTAAGGGGGGTGGGGGGTGGGGGGC TAGGGCAGGAAGGGGACTGGGCAAGTGCACACAGGCCCACACAGACACATGCACCCACACAGACACAGAG GGAACGGGCTGGGATGGGGACTGTGTGCACAGCAGGGCGGGTAGGACCTCCACAGCCCCTTCCCAAACAG CCTCTTATCCCTTACACCCCTTTCCTCCTCTCTTCCCCCATGGTAGGAACATAGCGTGTTTATATTTTAT GGCCAAACTATTTTGAATTTTGTTGTCTGGCCCTCAGTGCCCTGCCCTCTCCCTTTGCAGGACCACAGCT CCCGTCCTTTGGCTTCTGGTCCTTCCTGTTCTCCACCCTGGTTTCTTAAGTAGTTGGTTCTTCTCCTTAG TCTTCCTGACTCAACCCGTGGTCTCTGTCCCGCTCCCCCTCTCTGTGGCAGTTTCATATTTGCTAATACG AATTTGCTCATTAAACATTTTGTTGTATTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3593924 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3593924 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11182 same embryo
	EMAGE:11186 same embryo
	EMAGE:11181 same embryo
	EMAGE:11185 same embryo
	EMAGE:11184 same embryo
	EurExpress:euxassay_003614 same experiment
	MGI:4829044 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source