EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11403

Hspb3 heat shock protein 3 ( MGI:1928479)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006750. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006750_01	euxassay_006750_02	euxassay_006750_03	euxassay_006750_04

euxassay_006750_05	euxassay_006750_06	euxassay_006750_07	euxassay_006750_08	euxassay_006750_09

euxassay_006750_10	euxassay_006750_11	euxassay_006750_12	euxassay_006750_13	euxassay_006750_14

euxassay_006750_15	euxassay_006750_16	euxassay_006750_17	euxassay_006750_18	euxassay_006750_19

euxassay_006750_20	euxassay_006750_21	euxassay_006750_22	euxassay_006750_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11403_wholemount_strong.wlz

11403_wholemount_moderate.wlz

11403_wholemount_weak.wlz

11403_wholemount_possible.wlz

11403_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11403_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

moderate

regional

moderate expression: see section 01 02 03 04 05 21 22 23

upper leg muscle

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 20 21 22 23

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

forearm rest of mesenchyme

moderate

regional

moderate expression: see section 01 02

hand

moderate

regional

moderate expression: see section 01 02 03 04 05 06

foot

moderate

regional

moderate expression: see section 09 10 11

tarsus rest of mesenchyme

moderate

regional

moderate expression: see section 23

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

tongue muscle

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

tongue extrinsic muscle

moderate

regional

moderate expression: see section 09

left lung

moderate

spotted

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 weak expression: see section 04 05

right lung

moderate

spotted

moderate expression: see section 15 16 17 18 19 20 21 22 23

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8188
Entity Detected:	Hspb3, heat shock protein 3 ( MGI:1928479)
Sequence:	sense strand is shown >T8188 GTGAAAAGACAAGAGTCAGTGTGCCGGTCTGATTCAGCCCCAATTAAGCTGCCGTCTGGGCAGGACTGTA AACGGAGCTGCTAGGACTAAGAATCGAGTCCGCAGGCAACGCCAGAGACTAGACACTCACGGAAGGGAAG TGGTTCAACGCTGGAGTCGCCTCTGCTATGGCAAAAATCATTTTGAGGCACCTCATAGAGACCCCAGTGC GTTATCAGGAGGAGTTTGAAGCTCGAGGCTTAGAAGACTGCAGACTGGATCATACACTATACGCACTGCC CGGGCCAACCATCGAGGACCTGAGTAAAGCCAGAGGCGCAGGCACGCCACAGGCTCTGGCAGAGGACTCA GCCTCCACGGAGAAGCCACCCGGAGAAGGCAAATCCCGTTTCCAGATCCTGCTGGATGTGGTCCAGTTCC TGCCCGAGGACATCATCATCCAGACCTTCGAGGGCTGGCTGCTGATCAAGGCACAGCACGGAACCAGAAT GGACGAACACGGGTTTATATCGCGGAGTTTCACCAGACAGTACAAACTGCCAGATGGAGTCGAAACCAAA GATTTGTCTGCCATCCTCTGTCATGATGGAATCTTGGTGGTGGAAGTCAAAGATTCACTAGGGACCAAGT GAAAGCCTTTTGGTTGCTCTTCATACCCCAGGAAGGAAGATGAAAGCCAGGGACACCCCGGGAATGCTTA AACTTTTGAGATGGATTGCTGTCACTTTTATGAGGATTAAAAAAAAACCAAATGTAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAC
Notes:	The probe template was PCR amplified from IMAGE:851802 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:851802 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11405 same embryo
	EMAGE:11404 same embryo
	EMAGE:11402 same embryo
	EMAGE:11401 same embryo
	EMAGE:11406 same embryo
	EurExpress:euxassay_006750 same experiment
	MGI:4825481 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source