EMAGE mouse gene expression spatial database

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EMAGE:11543

B3gnt1 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1 ( MGI:1919680)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006738. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006738_01	euxassay_006738_02	euxassay_006738_03	euxassay_006738_04

euxassay_006738_05	euxassay_006738_06	euxassay_006738_07	euxassay_006738_08	euxassay_006738_09

euxassay_006738_10	euxassay_006738_11	euxassay_006738_12	euxassay_006738_13	euxassay_006738_14

euxassay_006738_15	euxassay_006738_16	euxassay_006738_17	euxassay_006738_18	euxassay_006738_19

euxassay_006738_20	euxassay_006738_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11543_wholemount_strong.wlz

11543_wholemount_moderate.wlz

11543_wholemount_weak.wlz

11543_wholemount_possible.wlz

11543_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11543_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 10

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16

pons mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 13 14 15 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 04 18 19 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 18 weak expression: see section 06 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 16 17 18 19 20 21 weak expression: see section 02

vagus x ganglion

moderate

regional

moderate expression: see section 06 07 17 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 16 weak expression: see section 06 07 17 18

spinal cord mantle layer

strong

regional

strong expression: see section 09 10 11 14 15 16 moderate expression: see section 08 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7060
Entity Detected:	B3gnt1, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1 ( MGI:1919680)
Sequence:	sense strand is shown >T7060 GCGGTAAATCCGGGCTTGCGGTGGCTGGCAGAGGCTACGGCCCGGTGGTCCCTGCTTCGTGCCGGGATCC CCGAGAGCCATGCAAATGTCCTACGCCATCCGATGCGCCTTCTACCAGCTGCTGCTGGCCGCGCTCATGT TGGTGGCAATGCTGCAGCTGCTCTACCTATCGCTGCTCTCCGGACTGCACGGCCAGGAGGAGCAGGAACA GTATTTCGAGTTCTTCCCGCCGTCTCCGCGATCCGTAGACCAGGTAAAGTCTCAACTCCGCACCGCACTG GCCTCCGGAGGCGTTCTGGATGCCAGCGGCGATTATCGCGTCTACAGGGGTCTATTGAAGACCACCATGG ACCCCAACGATGTCATCTTAGCTACGCATGCCAGTGTGGACAACCTACTACACCTGTCCGGACTTCTGGA GCGCTGGGAGGGTCCGCTGTCCGTTTCAGTGTTCGCGGCCACCAAAGAGGAGGCGCAGCTGGCCACGGTG CTGGCCTACGCGCTGAGTAGCCACTGCCCCGAGATGCGCGCTAGGGTCGCCATGCACCTCGTGTGCCCCT CGCGCTATGAGGCTGCTGTGCCCGACCCCCGAGAACCTGGGGAGTTTGCCCTGCTGCGGTCCTGCCAAGA GGTCTTTGACAAGCTAGCCAGGGTGGCCCAGCCCGGGATTAATTATGCACTAGGGACCAACACCTCCTAT CCCAATAACCTGTTAAGGAATCTGGCTCGGGAAGAGGCCAACTACGCCCTGGTGATTGATGTGGACATGG TGCCCAGCGAAGGGCTGTGGAGAGGCCTGAGGGAAATGTTGGATCAGAGCAATCACTGGGATGGCACAGC CCTGGTGGTGCCTGCATTTGAAATCCGCCGGTCCCGCCGAATGCCGATGAACAAGAACGAGCTGGTGCAG CTCTATCAGGTGGGCGAAGTCCGGCCCTTCTATTATGGGCTGTGCACGCCTTGCCATGCGCCCACCAACT ACTCCCGCTGGGTCAACCTGCCAGAGGAGAGCTTGCTGAGACCTGCCTACGTGGTGCCCTGGAGGGACCC CTGGGAACCATTTTATGTGGCTGGAGGAAAGGTGCCCACATTTGACGAACGCTTTCGGCAGTATGGTTTC AATCGAATCAGCCAGGCTTGTGAGCTGCACGTGGCAGGGTTTAATTTTGAGGTGCTGAATGAAGGTTTTC TGGTTCATAAGGGATTCAAGGAGGCATTGAAGTTCCATCCCCAAAAGGAGGCTGAAAACCAGCGCAATAA GATCCTTTACCGCCAGTTCAAACAGGAGTTGAAGGCTAGGTACCCCAACTCTCCCCACCGATGCTGAGTC CTTGTCTTCCATGGTCTGAGAATTGTCAGTATCTGGCTCCAGGCCTGACTGGGGTAAGAAACATGCCTCC ACTTTACAGAGGTAGCTATGGGTTTGGAACACTGGACTTGACTATGGGGTGCTGGGAGCCAAGTCTCAGC TTTACCACTAACTAGCTGTGTGGCCTTGAGCAAATACTATTACTTCTCTGAGCCTCCGTTACGCCGTCTG TAAAAAGGGAGGTGAAAATACCTACCTCACAGAACTGTTGGCTCAGATGAGACGCTGTATGTGTAAACAT CCTGTAAACTTCGTACAAATGTGAAGTATTATTAATATTGCTACAGTGTTATTATGATATTGTTACTATT AACCATGTTGGGTGGATAGCGGCAGAGCAAAAAGTTTGAATGGGCTAGAGGTGGGAAGCTTGAGTGTTTA ATAAAATGAGGCTTTTGGAAAGAAAGATGAAAACGTAGAATGTAGTGCTCAGAACGTTTCCCGTTCTCTC ATGCCTTCGCCCTTCGCCCTCCGCCCTCCGCCCTCCGAGATCCCTGAAGGAGGAAAGAGATAAAGGTTAA AGTCCCAGATCTGTGCCCTCCAGTAGGTTGGGCTGCTCATTTGCCTTCGGATGACATTATGTAAATAAAC GAATGTCACACCCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4010980 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4010980 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11544 same embryo
	EMAGE:11541 same embryo
	EMAGE:11545 same embryo
	EMAGE:11542 same embryo
	EurExpress:euxassay_006738 same experiment
	MGI:4823374 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source