EMAGE mouse gene expression spatial database

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EMAGE:11563

Bckdhb branched chain ketoacid dehydrogenase E1, beta polypeptide ( MGI:88137)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006740. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006740_01	euxassay_006740_02	euxassay_006740_03	euxassay_006740_04

euxassay_006740_05	euxassay_006740_06	euxassay_006740_07	euxassay_006740_08	euxassay_006740_09

euxassay_006740_10	euxassay_006740_11	euxassay_006740_12	euxassay_006740_13	euxassay_006740_14

euxassay_006740_15	euxassay_006740_16	euxassay_006740_17	euxassay_006740_18	euxassay_006740_19

euxassay_006740_20	euxassay_006740_21	euxassay_006740_22	euxassay_006740_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11563_wholemount_strong.wlz

11563_wholemount_moderate.wlz

11563_wholemount_weak.wlz

11563_wholemount_possible.wlz

11563_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11563_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8225
Entity Detected:	Bckdhb, branched chain ketoacid dehydrogenase E1, beta polypeptide ( MGI:88137)
Sequence:	sense strand is shown >T8225 CAGAATTGGATGGCTGAATTTGAACAGATCCTTCGGGAATTGAGACTTCAGGTCAACTCCACGCGCTTGG ACCTGTCGCTGACCAAAGGATTACCCAATTGGATCTCCTCAGCATTTTCTTTCTTTAAAAAATAGGGCAA ACTCAGAAGATGAACCTCTTCCAGTCAATAACAAGTGCCCTGGATAACTCATTAGCCAAAGACCCCACTG CAGTAATATTTGGTGAAGATGTTGCCTTTGGTGGAGTCTTCCGATGCACTGTTGGTTTACGAGACAAATA CGGAAAAGATAGAGTGTTTAACACCCCGTTGTGTGAACAAGGAATAGTTGGATTTGGCATTGGAATCGCG GTCACCGGTGCTACAGCTATTGCGGAAATCCAGTTTGCCGACTATATTTTCCCTGCCTTTGATCAGATTG TCAACGAAGCTGCCAAGTATCGCTACCGCTCAGGTGATCTTTTCAACTGTGGGAGCCTCACCATCCGGGC CCCGTGGGGTTGTGTGGGCCATGGGGCTCTCTACCATTCTCAGAGTCCTGAAGCCTTTTTTGCCCATTGC CCAGGGATCAAGGTGGTAATACCCCGAAGCCCTTTCCAGGCCAAGGGACTTCTGTTGTCATGCATAGAAG ATAAAAATCCATGTATATTTTTTGAACCTAAAATACTTTACCGGGCAGCAGTGGAACAGGTCCCAGTAGA ACCCTACAAGATCCCCTTGTCTCAGGCTGAAGTCATCCAGGAGGGCAGCGATGTGACTCTGGTTGCCTGG GGCACTCAGGTTCATGTCATCCGGGAGGTGGCTTCCATGGCCCAAGAAAAGCTTGGAGTATCTTGTGAAG TCATCGATCTGCGGACAATTGTGCCTTGGGATGTGGATACAGTTTGCAAGTCTGTGATCAAAACCGGGCG ACTGTTGATCAGCCACGAGGCTCCCTTAACAGGCGGCTTTGCCTCTGAGATCAGCTCCACGGTCCAGGAA GAATGTTTCTTGAACCTAGAGGCTCCAATATCTCGAGTTTGCGGATATGACACCCCGTTTCCTCACATCT TTGAGCCCTTTTATATCCCAGACAAATGGAAGTGCTACGATGCCCTTCGCAAGATGATCAACTATTGACG ACAGAGAAAACCAGGAAGATCATGACCAGACATGGAAATATTTTTCTGAAACCTTTTTATATTTCCTTAT ACTTCTTTCTTCTTACATATAGTTTTATGCAACGAACTGTCATGGATATTGGCTGATGAGCTGCGAATTA CTTATCATGTTAATATAAATGGATTGAGTGCACAGGAGGAATGCTTTAAGGAGTGCGTGGGCGGTGATCT TAAGACTGTTTCATGACTATAAATACCGGGTGGTGTTGTATTCAATAAAGCCAAATTACATCGCCTCAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1246285 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1246285 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11564 same embryo
	EMAGE:11562 same embryo
	EMAGE:11560 same embryo
	EMAGE:11561 same embryo
	EurExpress:euxassay_006740 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source