EMAGE mouse gene expression spatial database

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EMAGE:11610

Lrtm2 leucine-rich repeats and transmembrane domains 2 ( MGI:2141485)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006956. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006956_01	euxassay_006956_02	euxassay_006956_03	euxassay_006956_04

euxassay_006956_05	euxassay_006956_06	euxassay_006956_07	euxassay_006956_08	euxassay_006956_09

euxassay_006956_10	euxassay_006956_11	euxassay_006956_12	euxassay_006956_13	euxassay_006956_14

euxassay_006956_15	euxassay_006956_16	euxassay_006956_17	euxassay_006956_18	euxassay_006956_19

euxassay_006956_20	euxassay_006956_21	euxassay_006956_22	euxassay_006956_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11610_wholemount_strong.wlz

11610_wholemount_moderate.wlz

11610_wholemount_weak.wlz

11610_wholemount_possible.wlz

11610_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11610_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9166
Entity Detected:	Lrtm2, leucine-rich repeats and transmembrane domains 2 ( MGI:2141485)
Sequence:	sense strand is shown >T9166 TCTACCTCTCCCCCTCTTCGGTCTGTCTTCCTCCCGCCCTCCCTCTTTCGCTTCAGTTTGTCGCTGACCC CGCAGCCCCCTCCCCGGCAGCGGCAGCTCAGCCTGCGGGTCCGCCTCCTTCACAGGCGCTGCCCAGTCTC CCGCTGCCCTGAACCAGACCGGACATCCCTGCGTTCTGCCTGCCAAGCTGCCCGTGTGTCCGGCTACCTG GTCTGAGCATCAGGTCCCGGTGCAAAAAGGTCGTTGTGTCTCTCAGGCCAGCAGCAGAAGAGCAGGAAGC CCTGACCTGCGGGAGTTATAAATCGGCCAGCTGGCAGAAGACCAAGCTTGTAATTCACCTCAAGGTGATA GTGGGCTCAGAGGCAGGGGGTGGTGCAGCAGCTCACTCCCTACCTGTCAGCAGCAGCTACAAGGGAAGGG CCTCGCCTCTGCCTCCCCACAGCTATGGTAAGCTGGGCATTGGGGAGAATGACGGGACTGGTACATCTAG ATGGAGGGGCACAGGTGGGAGCTGGGGTCTCACTCATTTACCTAGGAGAGATGCACCTTCATTTCTCCCC TGTGGTAAGCTTGGGGTGGTGGTAGTGGTGGTGGAGGAAGTGATATTGCAAGGGGATAATTTTTAAAGCC CCCCAGAGTGCAAAGAGGCCTCAGGGGAGTGAGGGAAAGACAGGGGGTTTAGGGGGCTGAGCTGGGGTTC AGCTGGCAGTTCTTGTGCCTTCCAGCCCACTTCTAGGACTGCTAGATCCTCTTGACCCCCTTCTTGCAGG AGCAACAGGAGAGGGAGTGAAGAATTGTAAAGAGACACCTAGGCCTTGTCCCTGACCCCTGGGCTGGAAC GGGCATCAAATGCTTTGCTCTCTCTCTCAGGACTGACACGTGGCACATGCTGAGGGGCTCTCTCCAGAGC TGCGGAGAGTGTGGGTTTCACCATGTTGGCACCAGGCGGTGGCCCAGAGCAGAGGAGCAAGCTTGTCCTG CAATGGAGGCAGGTCTCCTGGATCACCTGCTGGATTGCCCTGTGTGCTGTGGAGGTGATCCCTGCCTGCC CCTTCTCCTGCACATGTGACAGTCGCAGCTTGGAGGTGGACTGCAGTGGCCTGGGTCTCACCACTGTACC CCCGGATGTGCCCGCGGCCACCCAAAGCCTTTTGCTCCTGAACAATAAGCTGAGTGCCCTACCAAGCTGG GCATTCGCCAACCTGTCCAACCTGCAGCGACTGGACCTGTCCAACAACTTTCTTGACCAGCTCCCCCGGT CCATTTTCGAGGACTTGGTCAATCTGACAGAGCTCCAGCTTCGGAATAACAGCATCAGGACCCTGGACAG GGACCTGCTCCAGCACTCCCCACTACTTAGACATCTGGATTTGTCTATCAACGGCTTGGCCCAGCTGCCC CCGGGGCTTTTTGATGGGCTCCTGGCTCTGCGCTCCTTGTCCCTCCGCTCCAATCGTCTGCAGAGCCTGG ACCGGCTGACGTTCGAGCCCCTGGCAAGCCTGCAGCTGCTTCAGGTTGGGGACAACCCGTGGGAGTGTGA CTGCAACCTTCGGGAATTCAAACACTGGCTGGAGTGGTTCTCCTACAGAGGGGGTCGCCTGGATCAGCTT GCCTGTACCCTACCCAAGGAGCTAAGGGGGAAGGACATGCGTGCGGTCCCCATGGAGATGTTTAACTACT GTTCCCAGCTAGAGGATGAGAACAACTCTGCTGGGCTAGATGCTCCAGGACCACCCTGCACCAAGGCCAG CCCGGAGCCTCCTAAACCTAAGCCTGGGGCTGAGCCTGAGCCCGAACCCAGCACTGCCTGCCCACAGAAG CAGAGGTACCGGCCCGTGAGTGTGCGGCGAGCCATTGGCACAGTGATCATCGCCGGGGTCGTGTGTGGCA TTGTCTGCATCATGATGGTGGTGGCCGCTGCTTACGGCTGCATCTACGCATCTCTCATGGCCAAGTACCA CCGTGAGCTCAAGAAGCGCCAGCCGCTGATGGGGGACCCTGAGGGTGAGCATGAAGATCAGAAGCAGATC TCGTCTGTGGCATGAGAGCTGTCTGTCTGGCCCAGGTAGGACAGCAAGGAGACTCCATGGGACTGCTACC AAAAGGGCTGCCCTTCCCTCCATCACAGGCCCCGTCTCAGCCTGACACTGGGACTGCCTCCCTCAGCTCC CTCGTCCCAATCTCTTTCCAAGTTTCCCAGAGGCTTCCCTCGGGCCAAAGATTCCCGTGGAAGGTCTTCT CTGGGCCTCTTGTCTTTGGAACCCCTGAGCCTCGCTGTAGGCTTCTGGCCACAGGGAGGAAAGAACCTAC TCTAGAGACATCATCTGCACTGAGCACTTCCCTCCTGCCCAGGGCTGCCAGAGCTGGTGACTCTCCCTAC CTCTTTCTCATTCCCACTTCAGTCTGCATTAGGGCCTGGGAGGGAGGGACAGGAGGCCCAAAGGTAGATT TCTGTGACTCCTTTTAAAGCCAGAAAGATGTCTCAAGTCTTTTGCAGTGTGAAGCTTGTCCAGCTCTTAG CCAATGTGAAACCACCCCAGTCCTGTGATCTGGGTCCAATGTTTAGAAAAACCAAAGTGGCTATAATGTC CCCCTTGGGTGGATGTCATTATGTCCCTTTATTGGCTATAGACTCCTCTGACTACATTGCTCCAGGTTTA CGAATTCTATCCATACTTTTTGTTCTAGCGTGAGGTTTTGAGACAGGGCCTTACTCTATATACTAATTCC TCTGTCAGGCAGGGCATCAAAGTCACACACACACACACACACACACACAGACACACACACACATCATGAG AACTTGGGGCAGAGGCCTGCTATCCCCTGAGCCCAGAGGCCACAGCCCCTCTTACTTCCTGACTACAGTC TTCCCATGTCATCAGGGGTTGGGTCCTGGACGACAACACAGGGATCCTTTGAGACCCTCTTAAGTGGGCC AAAGTATGCAGCTGGAGAAATTGCATTGTCCTGGCAGCCCTAGCACCTCTCTTGCTAGGTTTACCAGAAG GGTGCACATATGCATGACGGGTTCCAGGCGTCTGAGAGCTCTGTTGCAGACCCCCGGGCATGCCTAGGAA CCTCTTCCCTGCAGGTTCCAGGTACACGCAGCAGTTTCTACCCCTGCAGCCTGGCTTCTCCTGTCTCCTC TGTCATCTGCATCATTTGTGCATGACCTCAGAGACTGGGTCTGGGCTGTGCTCTGAGTTTGGTGTTTCAC CTTAAGGAAAGCAGATGTCAGGCAGTAAGTCCTTGAGAAGGCTGGCTGCCTTCAACACATCCCCGACAGA CTGCAGGAACAGGGACAGGATGGCTACATTCTGTTCAGAGTCCTTGACGGTGGGGCTTTCTTTCCCAGGG GTTCATCGGCTGCCTGGTTTCCTGGGAGTGTGCAATCGCACCTGGACTACCCAGGCTAAAAAAATGGCTG CAGTCCTATCTGGTTTGGAGATTCTTGCCCCTGGGCTGTGCACATTTTCAAGGTCACTGCTCTCTGTTCT AAACTGTCCCCTCCTTAGAGACTAGATACCTCTGTAGGAGATGCCAGACTGATACCTTACCCTGGGAAGG TCTTGGTAGTAGCCAGGAACACACTTAGGCACTGACTATACACCTATGAGGGCCTCAGGGCAGCCTCTCA GCCCTCCTTCTTGTCCTCGGCCACCTATTCGACTAGCAGGGGTGAGTACTGGAAGTCTCAGAAGAGCCTC CAAGGTTGTTCTCTGGCAGGTAGACAGAGAGCACCGGCTGCTGTTCAGGATCACAGGATCCTCTAGGCTG GGCCACAGTCTTCCTTCCTCTTTTGACTGGTGGGCCAATCCCAGAGCTGGGATTTTCTAAGGGCCTGGGG GAGGGAGGGTGTTGTCAGTGGTGATATCTTTAGCCTGAGACAGAAGATTTTTAAAGGCAAAATTATATTT CTGGTTTGTCGTTTCAGAAGACCAATAAAGACTATTTTCCTATGTAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827376 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827376 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11609 same embryo
	EMAGE:11608 same embryo
	EurExpress:euxassay_006956 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source