EMAGE mouse gene expression spatial database

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EMAGE:11696

Rnase4 ribonuclease, RNase A family 4 ( MGI:1926217)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006905. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006905_01	euxassay_006905_02	euxassay_006905_03	euxassay_006905_04

euxassay_006905_05	euxassay_006905_06	euxassay_006905_07	euxassay_006905_08	euxassay_006905_09

euxassay_006905_10	euxassay_006905_11	euxassay_006905_12	euxassay_006905_13	euxassay_006905_14

euxassay_006905_15	euxassay_006905_16	euxassay_006905_17	euxassay_006905_18	euxassay_006905_19

euxassay_006905_20	euxassay_006905_21	euxassay_006905_22	euxassay_006905_23	euxassay_006905_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11696_wholemount_strong.wlz

11696_wholemount_moderate.wlz

11696_wholemount_weak.wlz

11696_wholemount_possible.wlz

11696_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11696_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pancreas

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19 20 21

midgut

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 22

oral cavity

strong

regional

strong expression: see section 12 13 19

kidney calyx

strong

regional

strong expression: see section 09 10 11 12 19 20 21 22

ureter

strong

regional

strong expression: see section 13 14 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9956
Entity Detected:	Rnase4, ribonuclease, RNase A family 4 ( MGI:1926217)
Sequence:	sense strand is shown >T9956 ATCCCAACAGGAAGGAAGGAGTGAGAAACAAAGCTGCCGCCTCCAGTTCCGGGTCCAGGCACTTTCTAGG TAATGATGGATCTACAGAGGACTCAGTCCTTGCTTCTGCTCTTGGTGCTGACCCTGCTGGGGTTAGGGCT TGTACAGCCCTCCTATGGCCAGGATCGAATGTACCAACGGTTCCTTCGACAGCATGTGGACCCTCAGGTG ACAGGTGGCAATGACAACTACTGCAACGTGATGATGCAGAGACGGAAGATGACTTCTGTCCAGTGCAAAC GCTTCAACACCTTCATCCACGAAGACATCTGGAACATTCGTGGCATCTGCAGTACCACCAATATCCTGTG CAAGAACGGCCAGATGAACTGTCACGAAGGTGTAGTGAAGGTCACGGACTGCAGAGAGACAGGGAACTCC AAGGCCCCCAACTGTAGATACAGGGCAAGAACCAGCACTAGGCGAGTTGTCATTGCCTGTGAGGGTGACC CAGAGGTCCCAGTGCACTTTGACAGATAGATGCTGTCCTGCAGCTGTTACAGCTGATAGTTGACCTCTGA GTCAGTGAGACCAACAATGAGTGGTGGCCCCAGGCTTTGTTTCCTTTGCTCGTGCCTTATACTCACATAT ATCCAATATAACCCATGGTGTTAAGCGCATCCCATCCAAGGGAAGAAAGAACCCACTGCTCACAGCACTG ATGGCTTGGTTCTCCCAGATTCTATCCCCTGGAACTTATGCATTACATGTATTCAGATAGTATCTCAACG ATTATAGAGAGCCTTTTCTGTCCAAGCTGTCTCATTCCAGTCCTCTAACACCCCTTGAGACCAATTCTGC CACTGTATTTAGAAGCTTTGAGTTAAAGGATTTGACGACCACAAAAAACCAAAACCAAAACCAAAAACAA AAACCCAGTGGAAGATCTCAGACAAAAATCCAACTGGACGGGCTACTCACAAAAGGTGTTACCCACTTCG TTATATATACTTTCTGTTATTCCAAAATGTCATCATTAGGGAAGCCACTAAGAGTTTGGAAATAGGGGCT GGAGAGATGCCTCTGTGGTTAAGAGCATTGGCTGCTCCTCTAGAGGACCCAGGTTCAATTCCCAGCGCCC ACACATCAGCTCACAACTGTCTGTAACCCAGTTCTAGGTGATTTGATACCCTCACACAGACATACATGTA GACAGATCACCAATGCATATAAAATTAAAATAAAGATTTTGGAAACAACAATAAAAAAAAAAAAAAAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30296907 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30296907 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11694 same embryo
	EMAGE:11691 same embryo
	EMAGE:11693 same embryo
	EMAGE:11695 same embryo
	EMAGE:11692 same embryo
	EurExpress:euxassay_006905 same experiment
	MGI:4827752 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source