EMAGE mouse gene expression spatial database

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EMAGE:12149

Ecm2 extracellular matrix protein 2, female organ and adipocyte specific ( MGI:3039578)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011115. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011115_01	euxassay_011115_02	euxassay_011115_03	euxassay_011115_04

euxassay_011115_05	euxassay_011115_06	euxassay_011115_07	euxassay_011115_08	euxassay_011115_09

euxassay_011115_10	euxassay_011115_11	euxassay_011115_12	euxassay_011115_13	euxassay_011115_14

euxassay_011115_15	euxassay_011115_16	euxassay_011115_17	euxassay_011115_18	euxassay_011115_19

euxassay_011115_20	euxassay_011115_21	euxassay_011115_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12149_wholemount_strong.wlz

12149_wholemount_moderate.wlz

12149_wholemount_weak.wlz

12149_wholemount_possible.wlz

12149_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12149_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 11 15

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13 14

cerebral cortex ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 16 17 moderate expression: see section 04 18 19 20

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 04 05 06 13 moderate expression: see section 07 08 09 10 16 17 18 19 20 21 22

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 13

midbrain ventricular layer

strong

regional

strong expression: see section 12 13

spinal cord ventricular layer

moderate

regional

moderate expression: see section 14 15

anterior naris

moderate

regional

moderate expression: see section 12 17

heart valve

weak

regional

weak expression: see section 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9983
Entity Detected:	Ecm2, extracellular matrix protein 2, female organ and adipocyte specific ( MGI:3039578)
Sequence:	sense strand is shown >T9983 GGCTGATTTTCCACACAAAGAACTGAAAGGCGTTTATCCCAAGGGAAGCAGTTCTTTTAGATTTTACTAA GAAGTCAGCTAACACTTTTCAACATTCCCTTCTCTTCTTTGTTTTTAAAGAAAGTTCTGGTTTTGTTTCA CTTTCATCGGGAGACTTAAATGACACCAAGCAAAGCTACTTAGTTTAGATTTCCAGAAGTTCTTGGTGGA AAAAAAAAATCAAGCATGAAGCTAGCAGTGTTGTTTTGTTTCATTCTGCTTATTGTTCTTCAAACTGATT GTGAACGAGGTACTAGGAGGCAAAGGAGAAGGATGCATCAGAGAAGACTCAGGAAAAGCTCTTCATTCCA CCTCAGAGCCAACAGGCAGCTTGAAGTTCAGCAAACCACTGCTGCTCCAGATGCTAGACTACCCACTGCC AACTCTGATTACAGTGTGGAAGAAAACATAGAATCGCTTCTAAGTAATCTTGGAGTAGAATCAAGTTACA GTGTTTTACCAGGAAAGAAAGGATACTGCTTTGTAAAGGGCATGATCATGTACAACAAAGCTGTCTGGTC TCCTGAGCCCTGCACCACCTGCCTCTGCTCAAATGGAAGAGTGCTTTGTGATGAAACCGAGTGCCACCCC AAGGCGTGCCCCTATACCATTAAACCGGAAGGAGAGTGCTGCCCCATCTGCTCTGATGCTGAACAAGAAT CCATTAACAAACTTCACAAGCAAGTGCCACCTCCTCAGATGGAAATGGACCAAGTAGCCATAAAAGAAGC TCTTCAATCTGAGGAGGATGAAGAGATAGCTGAAGGACACAAGGAGCACAAGAAAGAGACCTCAGTGCCT ACTAAGATCCATGGCGATGGGGAAAGAACTGAGAGAAAACTGAGGCCGGAGAAAGAAGGGAGGTCAGCAC ATCAACCACTCTACCATGGAAGAAGGGAGGAAGAGGAGAGCAAGGAGGAGACTGAGAGGGAAGGAGAGGA GGAAGAGGAGGAGGAGGAAGAAGAAGAAGAGGATGCTATAAGAGGAGATGTGTTCAGAATGTCCTCCCGG GTAATTCCTGGAACCCCAAGAGGCAGGCCACGTCTGCCCAGAAGCTGTTCCCTGTCCTACAGGACTATCA GCTGCGTTCATGCAGACTTCACTGAGATCCCACCAATAACAGCACCAGAAGTAACGAACCTGGAGTTGGT TGGGAATTCGATCATCTCCATTCCGGATGAAGCATTTAATGGATTACCAAATTTGGAAAGACTTGATCTG AGCAGAAATAATATCACTTCTTCGGGCATAGGTCCAAAAGCATTCAAGTCTTTGAAGAAGTTAATGCGTC TGAACATGGATGGAAATAATTTGGTACATATTCCTTCAGACTTACCATCTACACTAGAAGAACTTAAAAT AAATGACAACAATCTCCAGGCTATCGATGAGAAAAGTTTATCAGACTTAAATCAACTTGTCACTTTAGAA TTGGAAGGAAACAATCTCAGTGAAATCAATGTCGACCCTCTAGCTTTCCAATCTTTGGAAAGTCTATCGT ATCTACGTCTGGGAAGAAACAAATTTAGAATCATACCACAAGGTCTTCCTGCTTCTACTGAGGAGTTATA CCTAGAAAATAATCAAATTGAAGAAATAACTGAAATTTGTTTCAATCATACCAGAAAGATAACTATGATC ATTCTACGTTACAATAAAATAGAAGAAAGTCGGATTGCTCCTTTAGCATGGATAAATCAAGAAAATCTTG AATCTATTGACCTGTCCTATAACAAGCTCTACCACGTCCCCTCCTACCTACCCAAATCTCTTCTGCACCT TGTCCTAATTGGGAACCAAATCGATCGGATCCCTGGCTATGTGTTTGGCCACATGCAACCAGGCCTGGAG TACTTGTACCTGTCATTTAACAGACTTTCTGATGATGGTGTGGACCTAGTCTCGTTCTATGGAGCATATC ATTCTCTGAGAGAATTATTTCTGGATCACAATGACTTCAAATCTATACCACCTGGAATACAAGACATGAA AGCACTGCATTTTCTGAGGCTGAACAACAATAAGATTCGGAATATTCATCCTGAGCAAATCTGCAATGCT GAAGAGGATGAGGACTCAGCTCTCGAGCATCTTCATCTTGAAAACAATTATATTAGAACTAGAGAGATAT CATCCTATGCATTTTCATGCATAAGACTGTATTCAAGTATTGTTCTTAAACCACAACACATCAAGTGATT TCAAGCTTCCTTCTGCCATTATTGTTTTTTGTTTGTTTGTTTTCTGAGACAGGGTTTCTCTGTGTAGCTC TGGCTGTCCTGGAACTCACTTTGTAGACCAGACTGGCCTCGAACTCAGAAATCCACCTGTTTTTGCCTCC CAAGTGCTGGGATTAAAGGCGTGCGCCACCACCGCCCGGCACCTTCTGCCATTTATAAATTTTACTGAAG CATTAACAAAATTACGTCTCTTTTATTGTGTGTGTGTGTTTCTGTATGTGTTTGTGCACATGAGTTCTGT GCTCTCAGTAACCAGAAGAAGGTGTTGGTTGCCCTGGAGCTGGAGTTACAAGTGGTTCTGAGCCAGTTGA TAAGTGTGCTGTAAAATGAACTTGGGTATGTGGTAAGAGCAGTACATGTTCTTAACTGCTTGATCCATGT CTCCAGTCTCCAAAACACCATTCTAATGCTAATTTCTTGGTTTAAATAATTTTACAGTGGTTACATATAC ACACTGGTAATGGTAAGGAAAGCTAGCAGAAGGGCATACAAAAATTCTACACTATAAGTCTAGAATTAGT TTTCAAAGTTAAAATAAAGCCATTTCAAGACTGCTTTCATTCTGCAGCAAGATAAAAAAATTAGAAAAAG TAAACTGAGCATGGTGGCACATGCCTTTAACCCCAGCGCTGTGAGACAGAGGCCAAAGGATCTCAGTGAG TATGAGGCCAGCCGGGTCTATATGGTGAGATCCAGGACAGCCTGAACTATGTAAAGACCCTATCTCAAAA AAAGACTACAAAATGTAAGCACTCTTCTAAAAATACAAATTACTGTTAATTATAAGCTGACATAAATGTA AATACAACTCATTCCTACACTAGTTATATAGTTAGCAACCATCTCTTTTGCCAGTTACTAAAATATACTT TTTATCAGTTTAATGAACAAAGTAGCACTCATTACACACTGACTTGTAATGATTTCTTTTAATGTAAACC TGATCGATCGATCGATCGATCGATCTATCTATCTATCTATCTATCTATCTATCTACCTACCTACCTACCT ACCTACCTACCTACCTACCTGTCTGTCTATGATCTAGCTATTAAGAGAGACTCTTACTGTATAGCTCTGG CTGGCCTGGCACTTAACCAGGTGCAAGGATTAAAGGCATGTGCAATCTTTGATCTCTGTATTTATACAAA ATTTTATATAATATATATAGAAACTCTGTTGATTTCTTTTATTAACTCTTAAATTATTGTTCTGAAGAAA TACTAGTGTCATGACTTAAGCTCTAGCTTTGGGATCTACCTTAGTTTCTTTTCCTGTTACTGTGATAAAA ATACCTCAATAAAAGCTAACTCAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30295705 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30295705 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12148 same embryo
	EMAGE:12147 same embryo
	EurExpress:euxassay_011115 same experiment
	MGI:4824467 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source