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EMAGE:12322

Trpa1 transient receptor potential cation channel, subfamily A, member 1 ( MGI:3522699)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12322 EMAGE:12322 EMAGE:12322 EMAGE:12322 EMAGE:12322
"Pseudo-wholemount" of euxassay_011045. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011045_01 euxassay_011045_02 euxassay_011045_03 euxassay_011045_04
EMAGE:12322 EMAGE:12322 EMAGE:12322 EMAGE:12322 EMAGE:12322
euxassay_011045_05 euxassay_011045_06 euxassay_011045_07 euxassay_011045_08 euxassay_011045_09
EMAGE:12322 EMAGE:12322 EMAGE:12322 EMAGE:12322 EMAGE:12322
euxassay_011045_10 euxassay_011045_11 euxassay_011045_12 euxassay_011045_13 euxassay_011045_14
EMAGE:12322 EMAGE:12322 EMAGE:12322 EMAGE:12322 EMAGE:12322
euxassay_011045_15 euxassay_011045_16 euxassay_011045_17 euxassay_011045_18 euxassay_011045_19
EMAGE:12322 EMAGE:12322 EMAGE:12322
euxassay_011045_20 euxassay_011045_21 euxassay_011045_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12322Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12322_wholemount_strong.wlz
12322_wholemount_moderate.wlz
12322_wholemount_weak.wlz
12322_wholemount_possible.wlz
12322_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12322_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
glossopharyngeal ix ganglion
moderate moderate
single cellmoderate expression: see section 06 07 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37560
Entity Detected:Trpa1, transient receptor potential cation channel, subfamily A, member 1 ( MGI:3522699)
Sequence:sense strand is shown

>T37560
AGTATCATCTTCGTGTTGCCCTTGTTCCTCAACATCCCAGCGTATATGCAGTGGCAATGTGGAGCAATAG
CGATATTCTTCTACTGGATGAACTTCCTACTGTATCTTCAAAGGTTTGAGAACTGTGGAATTTTCATTGT
TATGTTGGAGGTGATTTTTAAAACATTGCTGAGATCGACCGGAGTGTTTATCTTCCTCCTACTGGCTTTT
GGCCTCAGCTTTTATGTTCTCCTGAATTTCCAAGATGCCTTCAGCACCCCATTGCTTTCCTTAATCCAGA
CATTCAGTATGATGCTAGGAGACATCAATTATCGAGATGCCTTCCTAGAACCATTGTTTAGAAATGAGTT
GGCATACCCAGTCCTGACCTTTGGGCAGCTTATTGCCTTCACAATGTTTGTCCCAATTGTTCTCATGAAC
TTACTGATTGGCTTGGCGGTTGGGGACATTGCTGAGGTCCAGAAGCATGCGTCATTGAAGAGGATTGCTA
TGCAGGTGGAACTTCATACCAACTTAGAAAAAAAGCTGCCACTCTGGTACTTACGCAAAGTGGATCAGAG
GTCCACCATCGTGTATCCAAATAGACCCAGGCACGGCAGGATGCTACGGTTTTTTCATTACTTTCTTAAT
ATGCAAGAAACACGACAAGAAGTACCAAACATTGACACATGCTTGGAAATGGAAATATTGAAACAGAAAT
ATCGGCTGAAGGACCTCACTTCCCTCTTGGAAAAGCAGCATGAGCTCATCAAACTCATCATCCAGAAGAT
GGAGATCATCTCAGAGACAGAAGATGAAGATAACCATTGCTCTTTCCAAGACAGGTTCAAGAAGGAGAGG
CTGGAACAGATGCACAGCAAGTGGAATTTTGTCTTAAACGCAGTTAAGACTAAAACACATTGTTCTATTA
GCCACCCGGACTTTTAGTTCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 70437. Forward Primer - name:070437_F_cDNA_Anktm1, sequence:AGTATCATCTTCGTGTTGCCCT; Reverse Primer - name:070437_N_SP6_cDNA_Anktm1, sequence:AGAACTAAAAGTCCGGGTGGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12321 same embryo
 EMAGE:12323 same embryo
 EurExpress:euxassay_011045 same experiment
 MGI:4828937 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS