Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12398

Magi2 membrane associated guanylate kinase, WW and PDZ domain containing 2 ( MGI:1354953)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12398 EMAGE:12398 EMAGE:12398 EMAGE:12398 EMAGE:12398
"Pseudo-wholemount" of euxassay_011318. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011318_01 euxassay_011318_02 euxassay_011318_03 euxassay_011318_04
EMAGE:12398 EMAGE:12398 EMAGE:12398 EMAGE:12398 EMAGE:12398
euxassay_011318_05 euxassay_011318_06 euxassay_011318_07 euxassay_011318_08 euxassay_011318_09
EMAGE:12398 EMAGE:12398 EMAGE:12398 EMAGE:12398 EMAGE:12398
euxassay_011318_10 euxassay_011318_11 euxassay_011318_12 euxassay_011318_13 euxassay_011318_14
EMAGE:12398 EMAGE:12398 EMAGE:12398 EMAGE:12398 EMAGE:12398
euxassay_011318_15 euxassay_011318_16 euxassay_011318_17 euxassay_011318_18 euxassay_011318_19
EMAGE:12398 EMAGE:12398 EMAGE:12398 EMAGE:12398
euxassay_011318_20 euxassay_011318_21 euxassay_011318_22 euxassay_011318_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12398Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12398_wholemount_strong.wlz
12398_wholemount_moderate.wlz
12398_wholemount_weak.wlz
12398_wholemount_possible.wlz
12398_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12398_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37538
Entity Detected:Magi2, membrane associated guanylate kinase, WW and PDZ domain containing 2 ( MGI:1354953)
Sequence:sense strand is shown

>T37538
CATCATCAGCTCTCTGAACAGGCCTGAGTCTGGAGCCACCATAACTGTGCCCCATAAAATTGGACGAATC
ATTGATGGGAGCCCTGCAGATCGCTGTGCCAAACTCAAAGTGGGCGACCGTATCTTAGCAGTCAACGGCC
AGTCTATCATCAACATGCCTCACGCTGACATTGTGAAGCTCATCAAGGACGCCGGTCTCAGTGTCACCCT
TCGCATCATTCCTCAGGAGGAGCTCAACAGCCCAACATCAGCACCCAGTTCAGAGAAACAGAGCCCCATG
GCCCAGCAGCACAGCCCTCTGGCCCAGCAGAGTCCTCTGGCCCAGCCAAGCCCCGCCACCCCCAACAGCC
CAGTCGCACAGCCAGCTCCTCCCCAACCTCTCCAGCTGCAAGGACACGAAAATAGTTACAGGTCAGAAGT
TAAAGCGAGGCAAGATGTGAAGCCAGACATCCGGCAGCCTCCCTTCACAGACTACAGGCAGCCCCCGCTG
GACTACAGGCAGCCCCCGGGAGGAGACTACTCACAGCCCCCACCCTTGGACTACAGGCAGCACTCTCCAG
ACACCAGGCAGTACCCTCTGTCAGACTACAGGCAGCCACAGGATTTTGATTATTTCACTGTGGACATGGA
GAAAGGAGCCAAAGGATTTGGATTCAGCATTCGTGGAGGAAGGGAATACAAGATGGATCTGTATGTGTTG
AGATTGGCAGAGGATGGGCCAGCCATAAGGAACGGCAGGATGAGGGTAGGAGATCAGATCATTGAAATAA
ATGGGGAAAGCACACGAGACATGACCCACGCCAGAGCAATAGAACTCATCAAGTCTGGAGGAAGAAGAGT
GCGGCTGCTGCTGAAGAGAGGCACGGGGCAGGTCCCGGAGTATGGAATGGTACCTTCCAGCCTCTCCATG
TGCATGAAAAGTGACAAGCATGGGTCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 100004. Forward Primer - name:100004_F_cDNA_Acvrinp1, sequence:CATCATCAGCTCTCTGAACAGG; Reverse Primer - name:100004_N_SP6_cDNA_Acvrinp1, sequence:GGACCCATGCTTGTCACTTTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12394 same embryo
 EMAGE:12397 same embryo
 EMAGE:12396 same embryo
 EMAGE:12395 same embryo
 EurExpress:euxassay_011318 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS