EMAGE mouse gene expression spatial database

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EMAGE:12433

Dgkq diacylglycerol kinase, theta ( MGI:102918)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011121. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011121_01	euxassay_011121_02	euxassay_011121_03	euxassay_011121_04

euxassay_011121_05	euxassay_011121_06	euxassay_011121_07	euxassay_011121_08	euxassay_011121_09

euxassay_011121_10	euxassay_011121_11	euxassay_011121_12	euxassay_011121_13	euxassay_011121_14

euxassay_011121_15	euxassay_011121_16	euxassay_011121_17	euxassay_011121_18	euxassay_011121_19

euxassay_011121_20	euxassay_011121_21	euxassay_011121_22	euxassay_011121_23	euxassay_011121_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12433_wholemount_strong.wlz

12433_wholemount_moderate.wlz

12433_wholemount_weak.wlz

12433_wholemount_possible.wlz

12433_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12433_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9384
Entity Detected:	Dgkq, diacylglycerol kinase, theta ( MGI:102918)
Sequence:	sense strand is shown >T9384 CTCTGCCGAGGGCCCTCTCTACACCGCGGACCTGGCTTTATCGTGCGCTTGGAGCTGGGGCGCTCGGGTT GTGGATGGCGGCGGCAGCCGAGCCCGGTGCCCGGACCTGGCCGGGCAGTGGTTCCCCACGTCTTGGCAGC CCGGCCGGCAGTCCAGTGCTGGGCATCTCGGGCCGCACACGCCCGGGGTCAGGGCCGGAGCGGACTAGCA GAGCTATTGGCTCCGCAGCACCTGGCCACAGCTTTCGCAAGGTGACACTCACCAAGCCTACCTTCTGCCA CCTCTGCTCGGACTTCATCTGGGGACTGGCTGGCTTCCTGTGCGATGTCTGCAACTTCATGTCCCATGAG AAGTGCCTGAAGCAGGTGAAGACCCCGTGCACAAGCATTGCACCAAGCCTCGTCCGGGTGCCTGTAGCCC ACTGCTTTGGTTCCCTTGGTCTCTACAAGCGCAAGTTCTGTGTGGTCTGCCGCAAGAGCCTGGAGGTACC CGCATTCCGCTGTGAAGTGTGTGAGCTGCACGTTCACCCCGACTGTGTGCCCTTCGCCTGCAGCGACTGT CGTCAGTGCCACCAGGATGGACAGCAGGATTATGACACGTATCACCACCACTGGAGGGAGGGGAACCTGC CTTCTGGTGCACGATGTGAGGTCTGTAGGAAGACTTGTGGTTCCTCAGATGTGCTGGCTGGTGTACGCTG CGAGTGGTGTGGTGTACAGGCTCACTCAGTGTGCTCCACAGCACTTGCGCCTGAGTGTACATTTGGACGT CTACGCTCCATGGTACTGCCTCCTTCGTGTGTGCGCCTGTTGTCCCGAAACTTCAGCAAGATGCACTGTT TCCGAATCCCTGAGACCATGGTCCTGGAGCTTGGTGATGGGGATGATGGCGTAGACGGGAGTGCTGCTAT AGGCACAGGCAGAGAGGTACTGACAGCTACAGAGTCCACCAAACAGACCCTGAAGATCTTTGATGGCAAC GACTCCATGAGGAAAAATCAGTTTCGTCTGGTTACAGTTTCCCGCCTGGCTCGGAATGAGGAAGTGATGG AGGCAGCACTTCGGGCCTACTATATTAGCGAGGACCCTAAGGACTTCCAGCTGCAGGCACTGCCCCTGTC TGGCAATGCCCAGGCTCTGGGGAAGGCTGGGACCACTGAGGAGGAGGCTAGTAAAGGCTCTTGTCCCCGG GATTCCGTGCCTGAGGCCTGGGTCATCAGGTCTTTGCCTCGTACCCAAGAGATCCTGAAGATCTACCCTG GCTGGCTCAAGGTAGGTGTGGCCTACGTGTCCATCCGTGTGAACTCCCAGAGTACAGCACGGTCTGTGGT TCAAGAGGTTCTCCCACTGTTTGGACAACAGGTTGAGGATAAGGAGAGATTCCAGCTGATTGAGGTGCTC ATGAGCAGCAGACAAGTCCAACGGACCGTGCTGGCAGATGAAGAACCTCTGCTAGACCGACTCTGGGACA TCCGACAGACTTCTGTGCGCCAGGTGAGCCAGACGCGGTTCTACGTGGCCGAGACCAGGGCCACGGCCCC ACGTGTCTCCCTGTTCGTGGGTGGCCTGCCACCTGGCTTGTCCCCCCAGGATTACAGCAACCTGCTGCAT GAGGCCATGGCCACCAAAGCTGCTGTGGTGTCTGTGAGTCACGTCTACTCCTTACAAGGTGCAGTAATTC TGGACGTCACCTGCTTCGCGGAGGCTGAGCGGCTATACATGCTGGCCAGGGACACAGCAGTGCATGGCCG GCCACTGACTGCATTAGTGCTTCCAGATGTGCTGCACACGAAGCTGCCTCCTGACTGCTGCCCTCTCCTC GTGTTTGTGAACCCCAAGAGTGGGGGTCTCAAGGGACGAGAACTGCTCTGCAGTTTCCGGAAGCTGCTGA ATCCGCACCAGGTCTTTGAGCTAACCAACGGGGGCCCTCTTCCTGGGTTCCACCTTTTCTCCCAGGTGCC CAGTTTTCGGGTACTGGTGTGTGGTGGAGATGGCACCGTTGGCTGGGTGCTCGCTGCCCTGGAGGAGACA AGGCGCCATCTGGCCTGTCCAGAGCCATCTGTGGCCATCTTGCCCCTGGGTACAGGGAATGACCTTGGCC GGGTCCTTCGTTGGGGGGCAGGCTATAGTGGTGAGGACCCATTTTCTGTGCTGGTGTCGGTGGATGAGGC TGATGCTGTGCTCATGGATCGATGGACAATCCTGCTGGATGCTCATGAAATTGATAGTACAGAGAACAAT GTGGTAGAAACAGAGCCCCCCAAGATTGTTCAGATGAATAACTACTGTGGCATTGGTATTGATGCGGAGC TCAGCCTGGACTTCCACCAGGCACGTGAAGAGGAGCCTGGCAAATTCACAAGCAGGTTCCACAACAAGGG CGTGTATGTGCGGGTCGGGCTGCAGAAGATCAGCCACTCTCGAAGCCTACACAAGGAGATCCGGCTGCAG GTGGAGCAGCAGGAGGTGGAGCTACCCAGCATTGAGGGTCTCATCTTCATTAACATCCCCAGCTGGGGCT CAGGGGCCGACTTGTGGGGCTCTGACAACGACTCAAGGTTTGAGAAGCCACGCATAGACGACGGGCTGTT GGAGGTGGTGGGTGTGACAGGTGTCGTGCACATGGGCCAGGTACAAGGTGGGCTACGCTCTGGAATCCGC ATTGCCCAGGGCTCCTACTTCCGTGTCACACTCCTCAAGGCTACTCCAGTGCAGGTGGATGGTGAGCCCT GGGTTCAGGCCCCAGGTCACATGATCATCTCTGCTACTGCACCTAAGGTTCACATGCTGAGAAAGGCTAA GCAGAAGCCCAGGAAGGCTGGCGCCAACAGGGATACCCGAGTGGACACCTTGCCTGCTCCTGAGGGCAAT CCTTTATAGAGGAGCCAAGATGAGAGGTCTCAACCACCCAGCCTTTCTTTGGTTGGTCACACACTGGCTG GCCAGCAGATGGACTTAGCTGTAGCACTGACCATAGCATCTTCATGGGTCTTGGGATGTTTCCATCCCAA TGTCATGTGCAAACTTAGGAGTGGGTAGGTAATGGTCATTACTCACGCTGCCATACATAGTACCAATGTA TATGACTGGGCATGAGGCTCTGGCTAGCTTGTCTTCTGCACAAGACTAGTTCTTACAGGAAAAACGGAGC TGGTCCTGTTAGCCACATTCCTTCCTTTGAAACCTCTCTTGAAACCCTCTTAGACCCAATACGTCTGGAT GCAGGCCATCCAGGTGGGTCCTCTGATTTCATTTGTTCTTTCAAAGCAGCTAACCATGTTGGGCCCTGGC CTAACAGATCCAGAAAGCTGCCTGTTCTCCTGTACTGACTGGGTGGGTGTGGCATTCTCAAGTCCCCTGG ACTTATCACTAGATGTCAGTTTGGGCTTAACATACTAGTTACAAGGTCTCATCCAGCTGGTGCCCATGGA CTGACAAAGTTGTACTTAGGGATGGGAATCTTTGTCTACCCTTAGGCTAAGTGAGTTTAGTTATCACTCA CATGTCATAGCTGGAATGTGTGCTAGGCCCTTAGTGTCCCTCTTCAACTCCAGAGTGGGCACCTAGTGCC CAGAAGGGTTCAGAACAGGGCCAGGTAGTACCTGGTTGAGGAGTGTGTAAATAACATCTGTTGTGGAGTG GCTTGAACTCCCTTAGACTCAACTTATAGCACTTTATATACATGGTGTTTGCCTGCCTACAGCCCTTCCT TGTAGCTGTAGAGCTTCTACTCTGGCACCTACACAAATGGAAGCAGCTCTGATGGCCAGGTCTTGCTGGA GGCACCAGGTTCACAGAACGAGAGAAAAGCGGTCTCTTCCATGCACCCAAGAATTCATTGTCACACTTAA GACCCTAGAAAGAATTTGTCCAAAAATGAGCTGCCTAGCTAAGCCTGGTACATGCCTATAATTCATTTCT CCAGGCTAAAGCATAACTGGGTTATAGCGCTAAAGCCCCTCTCAAAAAGTGAAAACATTAAAAAAAAATG CTAAAGACATCTAAAGGTCTAGCCCCAGGCCTGGCTCGGGGAAGACTATGTGGGAAGTCTCCTGTGATGA GTACCCCCCACCCCCAAAAGCCAGTCAGTGCAGAGCCTTCTCCTGTGTGTCATTACTTGGCTTGTGGACA CCTTCAGAAGTGTGATACCAGGGTAGTGTGTGGACCAGGGTGTCTCCCTGCCTGACAGCTTCATGGGACC ATTGTGCCACCCCCCCCTACCCCACCCCCTGCTTCTGCCAAAACTGCATACAAGTGCATCAGATAGCCAT GTGTGAGCTCAGTGCCGATGATCATGTTTACATCATTTTCAATGTTCAAAAGAAATTCTTTGTGATGTAA TTTTTCATTTTTATTTCAATCTGTAGGAATTGTTTGTAAATTACGGTCAGTGTAGCATGTCCTCTGTATT TAAGTGAGACTGTCTTTATGTGGAGTGTGTCCTGTGCACTGACTGGTCCTGTCTTTGTGTGTAATTTATC ACTACCTGAATGTGATCTAAGTTATATCTGGGCCAAATACACAGTGTGGGTTTTGCTGTCCCATAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6855058 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6855058 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12437 same embryo
	EMAGE:12434 same embryo
	EMAGE:12436 same embryo
	EMAGE:12435 same embryo
	EMAGE:12438 same embryo
	EurExpress:euxassay_011121 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source