EMAGE mouse gene expression spatial database

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EMAGE:12485

Tbl2 transducin (beta)-like 2 ( MGI:1351652)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011278. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011278_01	euxassay_011278_02	euxassay_011278_03	euxassay_011278_04

euxassay_011278_05	euxassay_011278_06	euxassay_011278_07	euxassay_011278_08	euxassay_011278_09

euxassay_011278_10	euxassay_011278_11	euxassay_011278_12	euxassay_011278_13	euxassay_011278_14

euxassay_011278_15	euxassay_011278_16	euxassay_011278_17	euxassay_011278_18	euxassay_011278_19

euxassay_011278_20	euxassay_011278_21	euxassay_011278_22	euxassay_011278_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12485_wholemount_strong.wlz

12485_wholemount_moderate.wlz

12485_wholemount_weak.wlz

12485_wholemount_possible.wlz

12485_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12485_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9160
Entity Detected:	Tbl2, transducin (beta)-like 2 ( MGI:1351652)
Sequence:	sense strand is shown >T9160 GGCTGAGGTGGCAGCTGTCCCGCAGTCATGGAGCTGCCGCAGATGCCGGAGCTGATGGGGTTGTCCCTCC TGGTGGGTCTGCTGGCCTTGGTGGCCACGGCGGCTGTGGCGCGGGGCTGGCTGCGGGCCGAGGAGAAGCC CAGCCAGCCCGTCTGTCAGAAAGAAAACGAACCCAAGAAGTCAGGATCCAAGAAGCAGAAGCAGAATCAG CGGGTTCGTAAGGAGAAGCCTCAGCAGCACAGCTTCACGCACCCCCTTCTGGCTGCAGCACTGAAGAGCC ACAGTGGGAATATATCTTGCATGGACTTCAGCAGCAACGGCAAGTATCTGGCCACCTGTGCGGATGACCG CACTGTCCGTATCTGGAGCACTAAAGATTTCCTTCAGCGGGAGCACCGCAGCATGAGAGCCAACGTGGAG CTGGACCATGCCACCTTGGTACGCTTCAGCCCTGACTGCAGAGCCTTCATTGTTTGGCTGGCCAATGGGG ACACCCTCCGTGTTTTTAAGATGACCAAGCGGGAAGATGGGGGCTTTACTTTCACGGCCACACCAGAGGA TTTCCCTAAAAAACACAAAGCGCCCATCATCAACATCGGCATTGCTGACACAGGGAAGTTCATCATGACG GCCTCCAGTGACACAACTGTCCTCATATGGAATCTGAAAGGTCAGGTGCTTTCCACGATCAACACTAACC AGATGAACAACAGTCACGCAGTTATCTCCCCATGTAGCAGGTTTGTGGGTTCGTGTGGCTTCACCCCAGA TGTGAAGGTCTGGGAGGTCTGTTTCGGGAAGAAAGGAGAGTTCCAGGAGGTGCTGCGAGCCTTTGAACTG AAGGGTCACTCTGCATCTGTCCACTCTTTCGCTTTCTCCAATGACTCTCGAAGGATGGCCTCCGTCTCCA AGGATGGGACATGGAAGCTATGGGACACAGATGTGGAGTACAAGAAGCAGCAGGACCCCTACTTGCTACG GACAGGCCGCTTTGAAGAGGCGAGCACCATGCCTTGCCGCCTGGCACTCTCCCCTGATACCCATGTCCTG GCCTTGGCCACTGGCACCAATATTCATCTCTTCAACACACGCCGCGGGGAGAAGGAGGAGTACTTTGAGT GTGTCCATGGGGAGTGTATTGCTGACTTGACTTTTGACATCACTGGCCGCTTCCTGGCCTCCTGTGGGGA CCGTGCAGTGCGGCTCTTCCACAATACCCCTGGCCACCGGGCTGTGGTGGAGGAGATGCAGAGCCTCCTG AAGCGGGCATCCAGCGAAAGTACCCGCCAGAGGCTGCAGCAACAGCTAACCCAGGCCCAAGAGGCCCTCA AGAGCCTGGGTGCCCTGAAGAAGTGACTCTGGCTCCAGATGGGTCTGGCTGTCTGGCCCAGCAGCTCCTG CTCCCCAGGGGCCCCTGGGATGGAGACCTGGAGGAAACTCCCAGTTTTCTTCCTGGTGCCCCCTACTTTT TCCTATTGAAACTATTCTTGCATACTCAGATATCTCTCTCTCTGTTTCTCCCTTCTTGTTTGACTCTTCC CAGACTGAAAGATACTGTTTTCTCACAGGCCCTAGGGGTAGAATCTGTCTTCATCTAGCACGGAGAAGGG GAGTAGACAGAAGACAGACAGAGCAAAACCTTTGATGGTTTGGCAAAAGACCCTGACATCCAAAGAATTT TGTCAGTGTAGAGGGAAAACCCTAGGGTCACCAGGGACAAATTGCTGTTTTATAAGAGTTCAGAACGTGT TTTATAATGCTGCGTGGTGAGCCTCCATCTGGACCAGGCTCCTGATAGCAGCAAGAAGTAATTAAGGACT GGTCTTTGTTGGCATTGGTCTGTAATATCAGCACTATACAGGCAAAGGTGAGAGGATCAGGGGTTCAAGG CCAGCTTGGACTACATAGTGAATTCTAAGTCCTCCTGGGCTCCATTCATATGGAGAGAAACCTTGGCGAG ATTGTTTTTCCCTGGGCTTCAGTTTTCTTATTTGTAAAATGGGGAACGATGCTCTCTGTGGCCTTCCTGC AAAGATGCTGTGAGGCTAGGAGTGTTATAATGCTCCCACACATGGAGCATAACAGGTCTGTCCAGTGTCA TTACCTACCAGGTAAACACAGGATGCAGACGGTGTTCTGCAAGCAAACTAGGAAAAAACCACAGCCCTTG TGAAGCCAAGTTTCCTAGGGCTCTGCTTGCTCATTCATCTTGGATAAACATGGTGGTTTGGTTCCCGTGC CTTGAGAAGTAATGCCATCGCAAAAGGGTGTTCCTCTCTCAGGGACTTCTTGAACCCATCACTTCAGGGC CTGCTTCATGGGACCAGCTAGTGATGCTGGCTAACACAGACAGACAAGACTAGGCCTGCTGCCAAGGGTA AAAGCTGGTGGGAGGACCTAGAGGCTGGTGTCTGTGATGTGCTTTTTGTTCATTTACAGCTGCAGGGGAG GTTGTGGACAGGAGGCTTGACCGTAGTCAATCCAGGACAATAATGGGGAAAGAATGGAATCCACCCACTA AGAGACTGGGTCTCAGCCATGTACATTTGAAGACTGCTTGGACTGGTCTTTGTTGGTGAGGAAGATGGCA GGCATCTAGGAAGGCTTGAATCTAGCAAAAGGTTCCCATCCCAGTTAGTTTTTATCTCTCTTAAAAGTAG GGCCTCTTCCTGCCACCCTCAATGCTACAGGATGTTTATAACAAGGCACAACCCTGAAGTTGACCTTGAT GGCTCCCTTGTCACCCTCTCCTTCTACCACTGCTGCTACATGCAGATGGCCTGCAGCATCTGCAGCTCTC CCACTCTTCTGTTACCATCCCGGTCTTAGCCACTGTCCCGTAGTATATGGATAGTACCTCTGTTTCCAAC CACATTACTTCAAGTCAGTTTTCTACATAGTTTAGCCTGCTTTGGGAAAACAAAAATGGTATTTTGGATC ATGCCATTTCCAAAAGGCTTCCTTCTCTCTACACTTGGAATTAAACCACAAACTCTCTTTGTGGCCTGCG TAGTGTTGTGAAATTTAGCCACTCCTGTGATCTTTCTTTGCAATGGTCCAATCCCATTCTTCCTATTCTT TGCTAACCCAGGCATTTGGCAGGCTCTGGGTGTGGGCTGGTCCCTCTTGTGCTGCAAGCATTTTTCCTTT CTGTTGGCAGACTTGTCAGTGCGATTCAGTCCTTGTAAGTTTCACTGGAAAACAGTTGCCACCTCACCAA CTCCCTCCGAAGTAGCCATTTCCACCAATCCTGTCCCTCCTGGTGCCCTGCCGCTTTTCCTTTTGTATGA ATTGGTGCTTTCTGAAGCCATCTTGTCTGGCTATGTGTTTACTGCAGTGTCAGAGAAGGGCAGTGAGTGG GGGTTAAGAGTGGGAATTAGGCCCGTCACCTGGTTGTGCTATGGAAGGAAACGGTAGAGACTGCACTGGG CTGGTGGCCGTGTGTCCTGGGTGCACAGTGGGAGGGAGCCGCTACTGACTGATAAGCCGCAGAAGTTGCC AGTTGCAGAGAAGTTAGATGCTGGCACTGTACTTTGCAAGGAGGCATCCTTGTGGACTGCACCAAACCCT GACGGGGGAGGGCGCAGCTCATCAGTCTCATTGGTGCTGAAGGATTCTTCCTTACTTTTCCTTTTGCAGT CCTGGGAATGGAAGCCAGGGTCAGGTAGACAAATAGCTCTGCTGGTGAGCCATGCCTCAACCCCTCACTG GGAGATGCTAGACGAAGACTCTCCTCAGCCACAGCCCTAGCCCTCACTGGACAAATGCTTTGCCTCCAAG CCTCTTTGCAGCAGTTTGTGAAATTGCTGAAGAATGCGTTGGAACAGAACCCTTGTTGGTCTGCTTTGCT CTGGGTCCCGAGTCAACTAGCCAGGACAAAGTAAGCTCTCAATAAATGGGACTCTGACTAGAAGTAATGA CCAAGCTTTTGATATACTTTGTTTTCTGCACTCCGCTCTCTATTGTGGATTCAGGTTTCTGATAAGATCA GCCATATTGAAGACCATGTGGCCACACACGGATTTGGGCTTCTGACTACACAGCTGTGCCTCAGGCTTGT GTGTGCGTGTGGGGGTCTGGTGGGCATCTGCTCTGGTTGTCGGAAAGCATCTGAAAATGCGTTCTCTTGC TTCTGCACTCGTGTCTTGAGAAGTAACTGGTGCCGTGGCTGCACAGGGAAGGCAGTGAACCCTAGCAAGA ATGCTGAGCTGCTGAAGTCAGAAGTCTGTTCCCAAACTGGCCAATTCACTGGCCTGTGGCCTGGAGTCCT GTAATCACAGAGGCTGCCAGAGTTCGTCTCCCCACTTGTGGAATGGGAATAAATGTTGTTCTGTTAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827496 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827496 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12486 same embryo
	EMAGE:12484 same embryo
	EurExpress:euxassay_011278 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source