EMAGE mouse gene expression spatial database

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EMAGE:12648

Wdr36 WD repeat domain 36 ( MGI:1917819)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011586. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011586_01	euxassay_011586_02	euxassay_011586_03	euxassay_011586_04

euxassay_011586_05	euxassay_011586_06	euxassay_011586_07	euxassay_011586_08	euxassay_011586_09

euxassay_011586_10	euxassay_011586_11	euxassay_011586_12	euxassay_011586_13	euxassay_011586_14

euxassay_011586_15	euxassay_011586_16	euxassay_011586_17	euxassay_011586_18	euxassay_011586_19

euxassay_011586_20	euxassay_011586_21	euxassay_011586_22	euxassay_011586_23	euxassay_011586_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12648_wholemount_strong.wlz

12648_wholemount_moderate.wlz

12648_wholemount_weak.wlz

12648_wholemount_possible.wlz

12648_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12648_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 10 17 18 19

pancreas

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

midgut

moderate

regional

moderate expression: see section 09 10 11 14 15 16 17 18 19 20 weak expression: see section 12 13

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 12

metanephros

moderate

regional

moderate expression: see section 07 08 09 10 11 17 18 19 20 21

seminiferous cord

moderate

regional

moderate expression: see section 06 07 08 09 19 20 21 22

left lung

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 13 weak expression: see section 12

right lung

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31037
Entity Detected:	Wdr36, WD repeat domain 36 ( MGI:1917819)
Sequence:	sense strand is shown >T31037 ATGGCCGCTGGTTGATAAGCGCTGCGATGGATTGCTCTGTTAGGACTTGGGACCTTCCTTCTGGGTGCCT TATCGACTGCTTTTTGTTGGACTCAGCGCCTCTAAATGTTACTATGTCCCCAACTGGAGACTTTCTAGCA ACTTCCCATGTGGATCACCTTGGAATTTATCTATGGTCGAATATTTCCCTCTATTCAGTTGTCTCCCTGC GACCACTTCCTCCAGATTATGTCCCTTCCATAGTGATGCTTCCTGGTACTTGTCAAACCCAAGGTATGGA AGACTTGGAAGAAAAGACTGAGCCAAGTGATGAAATGATAGAGTATGAGTCGCCAGAACAGTTGAGTGAG CAACTGGTGACTCTCTCCCTTCTCCCTGAGTCACGCTGGAAAAACCTTCTGAATCTTGATGTTATCAAGA AAAAGAATAAACCCAAAGAGCCGCCCAAGGTTCCCCAGTCAGCTCCGTTTTTCATCCCAACAGTTCCTGG GCTTGTCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5346181), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 62799. Forward Primer - name:062799_F_IRAV36-39_H14_5730444A13Rik, sequence:ATGGCCGCTGGTTGATAA; Reverse Primer - name:062799_R_SP6_IRAV36-39_H14_5730444A13Rik, sequence:GGGACAAGCCCAGGAACT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12649 same embryo
	EMAGE:12647 same embryo
	EMAGE:12644 same embryo
	EMAGE:12646 same embryo
	EMAGE:12645 same embryo
	EurExpress:euxassay_011586 same experiment
	MGI:4829197 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source