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EMAGE:12649

Pla2g4b phospholipase A2, group IVB (cytosolic) ( MGI:2384819)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12649 EMAGE:12649 EMAGE:12649 EMAGE:12649 EMAGE:12649
"Pseudo-wholemount" of euxassay_011558. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011558_01 euxassay_011558_02 euxassay_011558_03 euxassay_011558_04
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euxassay_011558_10 euxassay_011558_11 euxassay_011558_12 euxassay_011558_13 euxassay_011558_14
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euxassay_011558_15 euxassay_011558_16 euxassay_011558_17 euxassay_011558_18 euxassay_011558_19
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euxassay_011558_20 euxassay_011558_21 euxassay_011558_22 euxassay_011558_23 euxassay_011558_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12649Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12649_wholemount_strong.wlz
12649_wholemount_moderate.wlz
12649_wholemount_weak.wlz
12649_wholemount_possible.wlz
12649_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12649_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31035
Entity Detected:Pla2g4b, phospholipase A2, group IVB (cytosolic) ( MGI:2384819)
Sequence:sense strand is shown

>T31035
TCTGGAGGCAGTTCGGAGGGCGTTGCAAGAGTTCCCGGCGGCGGCTCGCGACCTCAATGTACCTCGTGTT
GTGCCCTACCTGGATGAGCCCCCAAGCCCACTCTGCTTCTACCGGGATTGGGTGTGCCCCAACAGGCCCT
GCATTATCCGAAATGCTCTGCAGCACTGGCCAGCCCTCCAGAAGTGGTCCCTGTCCTACTTAAGAGCCAC
GGTGGGCTCCACGGAGGTGAGTGTGGCTGTGACGCCAGATGGTTATGCGGACGCGGTGCGAGGGGACCGC
TTTGTGATGCCTGCCGAACGCCGCCTGCCCATAAGCCATGTACTGGATGTGTTGGAGGGTCGGGCCCAGC
ACCCAGGAGTCCTCTATGTGCAGAAACAGTGTTCCAACCTGCCCACTGAGCTGCCCCAGCTTCTGTCTGA
CATTGAGTCCCATGTGCCCTGGGCCTCTGAGTCACTGGGGAAGATGCCTGATGCCGTGAACTTCTGGCTG
GGTGATGCATCTGCAGTGACATCCTTGCACAAGGACCACTATGAGAATCTGTACTGTGTAGTCTCTGGCG
AGAAACACTTCTTGTTACATCCACCCAGCGACCGGCCCTTCATCCCTTACAATCTCTACACACCAGCAAC
CTACCAGCTGACTGAAGAGGGCACTTTTAGGGTGGTGGACGAGGAAGCCATGGAGAAGGTACCCTGGATC
CCACTGGACCCCTTGGCTCCAGACCTGACCCAGTACCCCAGTTACAGCCAGGCACAGGCCCTTCACTGCA
CAGTGCGGGCCGGCGAGATGCTGTACCTGCCAGCTCTGTGGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4912251), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 34654. Forward Primer - name:034654_F_IRAV36-39_G18_Pla2g4b, sequence:TCTGGAGGCAGTTCGGAG; Reverse Primer - name:034654_R_SP6_IRAV36-39_G18_Pla2g4b, sequence:AACCACAGAGCTGGCAGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12647 same embryo
 EMAGE:12644 same embryo
 EMAGE:12646 same embryo
 EMAGE:12645 same embryo
 EMAGE:12648 same embryo
 EurExpress:euxassay_011558 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS