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EMAGE:12908

Snx12 sorting nexin 12 ( MGI:1919331)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12908 EMAGE:12908 EMAGE:12908 EMAGE:12908 EMAGE:12908
"Pseudo-wholemount" of euxassay_011668. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011668_01 euxassay_011668_02 euxassay_011668_03 euxassay_011668_04
EMAGE:12908 EMAGE:12908 EMAGE:12908 EMAGE:12908 EMAGE:12908
euxassay_011668_05 euxassay_011668_06 euxassay_011668_07 euxassay_011668_08 euxassay_011668_09
EMAGE:12908 EMAGE:12908 EMAGE:12908 EMAGE:12908 EMAGE:12908
euxassay_011668_10 euxassay_011668_11 euxassay_011668_12 euxassay_011668_13 euxassay_011668_14
EMAGE:12908 EMAGE:12908 EMAGE:12908 EMAGE:12908 EMAGE:12908
euxassay_011668_15 euxassay_011668_16 euxassay_011668_17 euxassay_011668_18 euxassay_011668_19
EMAGE:12908 EMAGE:12908 EMAGE:12908 EMAGE:12908 EMAGE:12908
euxassay_011668_20 euxassay_011668_21 euxassay_011668_22 euxassay_011668_23 euxassay_011668_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12908Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12908_wholemount_strong.wlz
12908_wholemount_moderate.wlz
12908_wholemount_weak.wlz
12908_wholemount_possible.wlz
12908_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12908_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31633
Entity Detected:Snx12, sorting nexin 12 ( MGI:1919331)
Sequence:sense strand is shown

>T31633
CTTGCCTCAGGCCTCTGTCCCCCACCCCCTTCGCCGGTACCGGGCTCTTCTTCGGAAAGATGTCGGACAC
CGCAGTGGCTGACACTAGGCGCCTTAACTCGAAGCCCCAAGACCTGACCGACGCTTACGGGCCGCCCAGT
AACTTCCTGGAGATAGACATCTTTAATCCACAGACGGTGGGCGTGGGCCGCGCGCGCTTCACCACCTATG
AGGTTCGCATGCGGACAAACCTACCCATCTTCAAGCTGAAAGAATCCTGTGTACGGCGACGCTATAGTGA
CTTTGAGTGGCTGAAAAATGAGCTGGAGCGAGATAGTAAGATCGTAGTACCACCACTGCCTGGGGAAGCC
TTGAAGCGGCAGCTCCCTTTCCGAGGAGATGAAGGGATCTTTGAGGAGTCTTTCATTGAAGAAAGGAGGC
AAGGCCTCGAACAGTTTATTAACAAAATTGCTGGGCACCCACTGGCTCAGAATGAACGCTGTCTACACAT
GTTCCTGCAGGAGGAGGCAATTGATAGGAACTACGTCCCTGGGAAGGTCCTTGGGGAAAAGGATTGCTGA
GTCTGGTGTGACCCTGGTCAGAGAGATCAGGATTAGATTTTGACAGGGGACTTGGAACCGACCTCAGTGT
TGAAGATCCTTGAGATAGATTAGCTCTCTAGCTGCTGAGCCCATGCTGGTAGCTGAGTAGCCCCTTTCCC
ACCTCGCCAGTGTCGGTGTCCTTGCTGCCTTTTGATTTTTATCCTCTGTTACAGATTTTTTTTTTAAAGA
TTTCTCCTTTCATTGTGCACAAGTGCTGAGACTCTGAGGCTCCATTTCTTCTGTGTATATATCCTGACTT
GGGGCTTTTATTCAGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3592627), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 10405. Forward Primer - name:010405_F_IRAV08-11_E02_Snx12, sequence:CTTGCCTCAGGCCTCTGT; Reverse Primer - name:010405_R_SP6_IRAV08-11_E02_Snx12, sequence:TGCTGAATAAAAGCCCCAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12909 same embryo
 EMAGE:12911 same embryo
 EMAGE:12912 same embryo
 EMAGE:12910 same embryo
 EMAGE:12907 same embryo
 EurExpress:euxassay_011668 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS