EMAGE mouse gene expression spatial database

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EMAGE:13656

Mir409 microRNA 409 ( MGI:3619396)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019167. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019167_01	euxassay_019167_02	euxassay_019167_03	euxassay_019167_04

euxassay_019167_05	euxassay_019167_06	euxassay_019167_07	euxassay_019167_08	euxassay_019167_09

euxassay_019167_10	euxassay_019167_11	euxassay_019167_12	euxassay_019167_13	euxassay_019167_14

euxassay_019167_15	euxassay_019167_16	euxassay_019167_17	euxassay_019167_18	euxassay_019167_19

euxassay_019167_20	euxassay_019167_21	euxassay_019167_22	euxassay_019167_23	euxassay_019167_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13656_wholemount_strong.wlz

13656_wholemount_moderate.wlz

13656_wholemount_weak.wlz

13656_wholemount_possible.wlz

13656_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13656_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 10 11 17 18

pons mantle layer

weak

regional

weak expression: see section 08 19

ventral grey horn

weak

regional

weak expression: see section 13 14 16 17 18

temporal bone

weak

regional

weak expression: see section 04 23 24

orbito-sphenoid

weak

regional

weak expression: see section 04 05 06 07 08 19 20 21 22 23 24

viscerocranium

weak

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70010
Entity Detected:	Mir409, microRNA 409 ( MGI:3619396)
Sequence:	sense strand is shown >T70010 GAATGTTGCTCGGTGAACCCCT
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-409-3p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13654 same embryo
	EMAGE:13657 same embryo
	EMAGE:13655 same embryo
	EurExpress:euxassay_019167 same experiment
	MGI:4826280 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source