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EMAGE:13740

Zfp558 zinc finger protein 558 ( MGI:1921681)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13740 EMAGE:13740 EMAGE:13740 EMAGE:13740 EMAGE:13740
"Pseudo-wholemount" of euxassay_019570. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019570_01 euxassay_019570_02 euxassay_019570_03 euxassay_019570_04
EMAGE:13740 EMAGE:13740 EMAGE:13740 EMAGE:13740 EMAGE:13740
euxassay_019570_05 euxassay_019570_06 euxassay_019570_07 euxassay_019570_08 euxassay_019570_09
EMAGE:13740 EMAGE:13740 EMAGE:13740 EMAGE:13740 EMAGE:13740
euxassay_019570_10 euxassay_019570_11 euxassay_019570_12 euxassay_019570_13 euxassay_019570_14
EMAGE:13740 EMAGE:13740 EMAGE:13740 EMAGE:13740 EMAGE:13740
euxassay_019570_15 euxassay_019570_16 euxassay_019570_17 euxassay_019570_18 euxassay_019570_19
EMAGE:13740 EMAGE:13740 EMAGE:13740 EMAGE:13740
euxassay_019570_20 euxassay_019570_21 euxassay_019570_22 euxassay_019570_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13740Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13740_wholemount_strong.wlz
13740_wholemount_moderate.wlz
13740_wholemount_weak.wlz
13740_wholemount_possible.wlz
13740_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13740_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55226
Entity Detected:Zfp558, zinc finger protein 558 ( MGI:1921681)
Sequence:sense strand is shown

>T55226
GCGATAAAACCCGAGCTCTTCCAGCTCGCAGCCCTGGGAATGCTTTTTGTTCTCACAGAGGGGGGCGTGG
CAGGCGAGCTGAGGGATGGTCAGCCAGCGGGTCCTGTTCTATCTCCTGAGCTGCAAGACAAGAAAAACAT
CATGCAAACTCAAACTTGTGTTGGACCCTCAATGTTGTGTTGAAAACGCCTGAATGTCCACCTGCCCTCA
TTAATCTATTAATTTCATGTAACCAGAGTGCTGTCCTTTGTATCTGTGGGAATCAGAAGTGTATCTGCCC
CATCCACTGCCCTGTGTTCAGGAGCTCTAGTGTTGGAGTTTGGATGGCAGCAACTATCATTCCTCTAATA
TATGCTCAGTCTTCTCTGTACCCACTGTCTCCTGAGGAAGGACAGATGGGGA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GCGATAAAACCCGAGCTCTT; Reverse Primer - name:unspecified, sequence:TCCCCATCTGTCCTTCCTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13741 same embryo
 EMAGE:13743 same embryo
 EMAGE:13742 same embryo
 EurExpress:euxassay_019570 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS