EMAGE mouse gene expression spatial database

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EMAGE:13773

Stat5b signal transducer and activator of transcription 5B ( MGI:103035)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019576. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019576_01	euxassay_019576_02	euxassay_019576_03	euxassay_019576_04

euxassay_019576_05	euxassay_019576_06	euxassay_019576_07	euxassay_019576_08	euxassay_019576_09

euxassay_019576_10	euxassay_019576_11	euxassay_019576_12	euxassay_019576_13	euxassay_019576_14

euxassay_019576_15	euxassay_019576_16	euxassay_019576_17	euxassay_019576_18	euxassay_019576_19

euxassay_019576_20	euxassay_019576_21	euxassay_019576_22	euxassay_019576_23	euxassay_019576_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13773_wholemount_strong.wlz

13773_wholemount_moderate.wlz

13773_wholemount_weak.wlz

13773_wholemount_possible.wlz

13773_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13773_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 13 weak expression: see section 12 14 15

telencephalon mantle layer

moderate

regional

moderate expression: see section 10 11 17 weak expression: see section 16

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 21 22 23 24 weak expression: see section 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55193
Entity Detected:	Stat5b, signal transducer and activator of transcription 5B ( MGI:103035)
Sequence:	sense strand is shown >T55193 GCGTGAGTGTGAGTGCATTTGCACGTCGTGTTTTTTCCCCGCCCCTGCTGTCCAGTCTAAGCCGCCACGC CAGGGCAGCGGCTGCGCTTTTTTTTACCATGTGCAAAAAGGCAGTTGGTTCCCTGAACCCTGGAACCTGG CCATGTGTCTTCAGGGTGGCTGACCCTTGACACGTGACTATCCAAGTAAGAAAAGGACAGAGGAAAAAGC ACCCTCTCTCTGGGGAGCCTCGGTTCCTCTGCCAGGTAGTCCATAGTCCAAGCAAGCATTGTCATTGTCT CCGCCTGTCTTCTGAGATGTAGATGACTGTCTGATGATGAAAGCCAGTACCTCCCGTGTCCCCTGTCCCC TTTGCATAAGGGACGGAAAGGGGAGCTGAATCAAGGGTGATGGGGCAAGGTGGTCACAGGTTTTTGGATG GGGAGTGGCTGTTTCCCGTTTCTGCCACTTCCGCCATCTTAACACTGGCTCCTTCCCTCTCTGCTTGCTC AGTCTCTATTTCTAGAACTGCCACTCAGCTTAAGTGCAAGTGTGTGTACTCAAGTGGAGATGTTTAACAA AATAGTGGAGAGGAAGCCAGGCCACCCAGCTCTGAGCGTACAGGTTCAGGTGATGCCCTGTGTTCCTTCT GTCAGGGCGGTGCGTTGTGCCCAAGTCCTGGCTCCAGACACTGGGCGTAGCCTGTCTGCGCCAGCCTCCC CAACTCTTGTCTGTGCTGTGGCCAGGCCGCGCCTGCGCTATCCAAGGCTTTTCTCCAAGCGTGTTGATAA TGGCTTCCTGCAAACGTCCGGTGGTGTTTTTTGTTTCTAAATCAGGTCTTTTTTTATGTTTTTCCCATTT GCACCCTAATTTGACATCAAATTTCCCCCCTCCTGTATCAGGTCCTGGGTCCTCTGTACCCAGATCACTT CATCTCCCTTCAGTGTCACATAGTGCCCTGAGGATTAGGTGGTAGGAATGGGACCTGCACACGGGGCCAG CCTGCCAAGCAGGCAGCCAGCACTGTACAGTGCTGGGTGCCGGGTGGCCGTTGGGGATTGGGGAAATGCA GTCAGTCAGCGGGTTTCCTAGGAAGCTTGGAAAACTAAAAGCAAAGTGAAAGCCTCAGGGTGATTTGTTC CACAGTCTCCTCTGTAGTGTCTCCAGAAGGAAGGAAGGGGCTGCAGTGGGCCGTCAGGGAGAGGGGCAAG CAGAGAGCGGTTACCACTCAGGCTTGCTGAGAGCCCTCCTTGGCTTCCTCTCCCAAACAAGGGCAGAACC GTGCCCAGGAGAGGAGCCCCCAAAACCTTATTTTTATACATGCAAGTAAATAAACATATTTTTTTTACAA AAATAACTTCTGAATTTATCAGTGTTTTACTGTTAAAAGAAAATACTCCTGTGTAGTAAATTATTTATTG GGAGATGAGTTTTTAAAAGCTGCTGTTTGCCTTGCCTTGGTTTTGTACACTGATTTTTCTATGCCTGGCG GTAGCCTCTCTGCCTCAGGTGCTGGCCGGATGGAGGAGGTGTGAGGCCCCTCCCTGGCCCCTCAGAAGAA AGCTGGAACTGCCAGGGGAGTCCAGGCTTAAGGGACTCGTCCCCACCTGTCATGCGACTGTCCCAGTAAC CCTCACGAGGGTGTGGACTCGACAAATATCTAGATATATGGTGGACATGGCCCCAAGTCATGGGGAGAGT AGAGCAGCATGGG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GCGTGAGTGTGAGTGCATTT; Reverse Primer - name:unspecified, sequence:CCCATGCTGCTCTACTCTCC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4828489 same experiment
	EurExpress:euxassay_019576 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source