EMAGE mouse gene expression spatial database

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EMAGE:13819

Msx2 homeobox, msh-like 2 ( MGI:97169)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019605. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019605_01	euxassay_019605_02	euxassay_019605_03	euxassay_019605_04

euxassay_019605_05	euxassay_019605_06	euxassay_019605_07	euxassay_019605_08	euxassay_019605_09

euxassay_019605_10	euxassay_019605_11	euxassay_019605_12	euxassay_019605_13	euxassay_019605_14

euxassay_019605_15	euxassay_019605_16	euxassay_019605_17	euxassay_019605_18	euxassay_019605_19

euxassay_019605_20	euxassay_019605_21	euxassay_019605_22	euxassay_019605_23	euxassay_019605_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13819_wholemount_strong.wlz

13819_wholemount_moderate.wlz

13819_wholemount_weak.wlz

13819_wholemount_possible.wlz

13819_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13819_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 09 10 11 18 19

vibrissa

moderate

regional

moderate expression: see section 09 weak expression: see section 10 11 12 24

lower jaw incisor

moderate

regional

moderate expression: see section 13 weak expression: see section 14 18

lower jaw molar

moderate

regional

moderate expression: see section 09 weak expression: see section 21

upper jaw incisor

weak

regional

weak expression: see section 15 19

upper jaw molar

moderate

regional

moderate expression: see section 09 weak expression: see section 21

genital tubercle of male

moderate

regional

moderate expression: see section 15 16 17 18 weak expression: see section 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55112
Entity Detected:	Msx2, homeobox, msh-like 2 ( MGI:97169)
Sequence:	sense strand is shown >T55112 CCCCCTTTCCTCTCACAAATATTGGCTCTGCTAGTTTTTATGTATAAATATATAATAAAATATAAGACTT TTTATATGCCAGATGTAAAAATTCAAGTTATTTTAAAAGGCAAAATTTATATATACATTTATCCATTTTC TTTTTTTTTCTAGATAGCATCTTCCTAAGATATTGTGTTAAGTCCATTTGCTACATTTTCTTAAAGGGAA AGAGATTAATTGCAGAATTTGCAAGGATGGTGGACTTGCTTATGGAAAACATAGCAGAAGTAGACACATT CCATACAGGATGTCACTTCAGACAGATTCTTGCAAGTGAAGGGGGAGGTGTAACGTGTTTATTTGCATTT CTGTGCACAAGAAAAAGAAATCTCTTGATGCACATTTGATCCTATTTTTTTTCTCTTTTTCTCCATTCCA TATTATTAAATCTTTGTTGTTGTTTTAAGATCGATGAAAAGAGGATGAGAATAACTCTGAGAGTTACTCA GGGTAAAGTAGAAAATAGGAAACTCCATGTCAGGTCCCTGGAGTGAGAGGGGGTTAGAAGGTGAAATATT GTTTGTAAGAATTGATTTCTATGAAATGCTATCTACATCAGTCCTCACACGGTACAGAGGTGATTGGAAG AGGACATGGTAAATCTCTTCTTAAAAATGAGTGTCTTATAACTGTGGCCTATATTTTAAATATTCTTCAA CAGTCCATAGAATGAGGAAGGAAAACATGCCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC TCTCTCTCTAGCTCTCTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTACT TTGTGTGCTAAACTTCTTAGGGAAATTTATATACTCTAACGTGTTGGGCAGATGGAGAAGCCATGTGCTT GAGTTGCTGGAGATGGCGTTGTCAGTCAGCACCTGGGTGTCTC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CCCCCTTTCCTCTCACAAAT; Reverse Primer - name:unspecified, sequence:GAGACACCCAGGTGCTGACT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13818 same embryo
	EMAGE:13817 same embryo
	EurExpress:euxassay_019605 same experiment
	MGI:4826475 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source