EMAGE mouse gene expression spatial database

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EMAGE:14139

Cd300ld CD300 molecule-like family member d ( MGI:2442358)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001837. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001837_01	euxassay_001837_02	euxassay_001837_03	euxassay_001837_04

euxassay_001837_05	euxassay_001837_06	euxassay_001837_07	euxassay_001837_08	euxassay_001837_09

euxassay_001837_10	euxassay_001837_11	euxassay_001837_12	euxassay_001837_13	euxassay_001837_14

euxassay_001837_15	euxassay_001837_16	euxassay_001837_17	euxassay_001837_18	euxassay_001837_19

euxassay_001837_20	euxassay_001837_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14139_wholemount_strong.wlz

14139_wholemount_moderate.wlz

14139_wholemount_weak.wlz

14139_wholemount_possible.wlz

14139_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14139_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4934
Entity Detected:	Cd300ld, CD300 molecule-like family member d ( MGI:2442358)
Sequence:	sense strand is shown >T4934 GGCAACTCAAGCTGAGCTGCGAGTCCTCACAGGGTCCTGACATCTGTCGTCAACAAGGACATGAGAGGAG ACGACCATGTGGCAGTTCTCTGCTCTACTCCTATTCTTCCTCCCAGGCTGCTGCACGGCTCAGGATTCAG TCACAGGTCCAGAGGAGGTGAGCGGTCAGGAGCAGGGCTCCTTGACAGTGCAGTGCAGATATTCCTCATA CTGGAAGGGTTACAAGAAGTACTGGTGCCGAGGAGTTCCTCAGAGATCATGTGATATTCTTGTTGAAACC GATAAATCAGAGCAGCTGGTGAAGAAGAACCGTGTGTCCATCAGGGACAACCAGAGAGACTTCATCTTCA CAGTGACCATGGAGGATCTGAGGATGAGCGATGCTGGCATTTACTGGTGTGGAATTACGAAAGGTGGACC TGATCCCATGTTTAAAGTTAATGTGAACATTGACCAAGCCCCAAAAAGTTCAATGATGACCACCACAGCC ACAGTTCTGAAATCCATACAACCAAGCGCTGAGAACACTGGCAAGGAACAAGTGACTCAGAGCAAAGAAG TGACTCAGAGCAGGCCCCACACCAGGTCCCTGCTGAGCAGCATCTACTTCCTGCTGATGGTCTTTGTGGA GTTACCCCTGCTCCTGAGCATGCTCAGTGCTGTCCTCTGGGTGACCAGGCCTCAGAGATGCTTTGGGAGA GGTGAAAATGACCTGGTGAAGACCCATAGTCCTGTTGCCTAGGATAGAGAGAAACAGTTCCCAAGAAATG GAAAATAATCTCTGTCTCTCTGTTGTCTCTGTCTCTGTCTCTGGGGTGTATGTATGTGTGTGCATGCACC TTGCCGGGGCAGATGTGTATGTGGGAGACATCTACTGGAATCATTCCCTTAGTATCTGAGACAGGGTTTC TAATTGACCAGCACCTTTGTGTGGTAGGTCAGACAGCTGGCCAGGGAACTCCAGGGATCTCCCTGCCTCT ACCATCCATCCTGAGATTGCAAGCATACACGAGTGCCCTAGCTTAAAAACAAACAAACAAACAAACACCT TAGGTTGTAGGGATTGAACTCATGTCCTTGTACCTGCAAGGAAGGTAGGCGATTTACCTGCTGAGCCATC TCCCCAATCTGGAGAAGACTCAATCTAGTAAAGAACAACTCATCAGCAGTACCATGTCTCTGATGTGCTG CACAACCAGACTCAGACTAATCCCACTCCTATAGCAGGGACAGCTGAGTTCTGGAACCCATTCATGTGCC CCTCTCTCAGGACATCCTGCAATACCTATCTGGGGCTATCTTCCACTGATGACTTCCAAAGAAGAAAATA CAAGAAAACATCACATTTCTTCTTAGTGTACTAGTTCCTTAGAGGACACATGCCAATATAAGACTGCGGG CCACCAGCCAGTTGATTGACCAAATATCTCGGTGATGTGGCCTCACCAAGTAGCATAAAGTTTGCCACTG TCACACTAGCTATCTGTCCCTTATTGGCAGGACACACCCTGCTTTCTTTTTTCTCAACACAGCCCAGTGA CTAAGCCCATTGCAAACCCAGATGGAGTAGTTGACCTAAGCTTTGTACCACCTGCTCAGGTCTTCAAGTA GTAGTTAAGCCTTGGTCCCTGAAATCTAGATTGCTCAGTGAGACCAAATGGGGAGGTCAACTGCAGGAAT CAGCTGATCTCACAGGAGTCACGAACCCACATCACCCCCAAACCCTTCCAGGAATGGTCTCTTCACCAGG CCCTTCCACTCTCTCCCTTTTACTCAGACAAATCTATTGAATGTCTAAGTAGTTATCACTCTCCACATAC ATGCTCCAAAATAAGACAGACCCAATTAAAGTCCATAGAGAAGGCCAATGGGATCAAAGGTAAATACTCA GGGGAAATGAGTAGTCTCAGCCCACCAGTCTCAGACATCCTGAGTTCTGCACCATGACACAGTCTTCTTC TTGAGTGGGGCTCTGACACCCACAGCCAAATTCACAACTAACATGGGTGTTCTCCAACTTTGTGGAAGAA GAGTCCCCAGGTTAGCATCTTCTCAGTGATGACATGTGTTGGACTCTAGTGAGCTTGCCTCTTGTTAAGA GGATGGTTTTCATTTGCTTCAGGGGTATACCTGCCAGTCAGTCAGCCACATTCCCACTCATGCTCAGACC AACAATCATGGTTAAACTCTGTGGGACACACACACACACACACACACACACACACACACACACACACACG ACATATAATCAGGAGAGGGACTCATTAGAGCCTGTAGGTCAGGCAGTGGTAGCACATGCCTTTAATCTCA ACACTCAGGAGGCAGAGGCAGGTGGATTTCTGAGTTCTAGGTCAGTCTGCTTTACAGAGTGAGTTCTAGG ACTACACAGAGAAATCCAAAAAAACAAGGCTACACAGAGAAACCATGTCCTGGGGTAAAAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5125383 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5125383 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14138 same embryo
	EMAGE:14140 same embryo
	EMAGE:14141 same embryo
	EMAGE:14137 same embryo
	EurExpress:euxassay_001837 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source