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EMAGE:14267

Plk4 polo-like kinase 4 (Drosophila) ( MGI:101783)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14267 EMAGE:14267 EMAGE:14267 EMAGE:14267 EMAGE:14267
"Pseudo-wholemount" of euxassay_003979. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003979_01 euxassay_003979_02 euxassay_003979_03 euxassay_003979_04
EMAGE:14267 EMAGE:14267 EMAGE:14267 EMAGE:14267 EMAGE:14267
euxassay_003979_05 euxassay_003979_06 euxassay_003979_07 euxassay_003979_08 euxassay_003979_09
EMAGE:14267 EMAGE:14267 EMAGE:14267 EMAGE:14267 EMAGE:14267
euxassay_003979_10 euxassay_003979_11 euxassay_003979_12 euxassay_003979_13 euxassay_003979_14
EMAGE:14267 EMAGE:14267 EMAGE:14267 EMAGE:14267 EMAGE:14267
euxassay_003979_15 euxassay_003979_16 euxassay_003979_17 euxassay_003979_18 euxassay_003979_19
EMAGE:14267 EMAGE:14267 EMAGE:14267 EMAGE:14267
euxassay_003979_20 euxassay_003979_21 euxassay_003979_22 euxassay_003979_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14267Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14267_wholemount_strong.wlz
14267_wholemount_moderate.wlz
14267_wholemount_weak.wlz
14267_wholemount_possible.wlz
14267_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14267_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 17 18 19 20
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 14 15 16 17 18 19 20
cranium
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 23
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
clavicle
strong strong
regionalstrong expression: see section 07 08 09 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4991
Entity Detected:Plk4, polo-like kinase 4 (Drosophila) ( MGI:101783)
Sequence:sense strand is shown

>T4991
CGGACGCGTGGGGGAATTTTTCAAAATGGGAGCTCCGGGGCGCCGCCCAGGCCTCGGGAGGTACCGGGGG
TACCTTTCGGTGGCGTTGGCGGCGTCGCCAGCGGCGGCGTAGAGAAGGCGTCCTGATGGGCGCCAAGACC
TGCTGGCTTCTCGGAGCGCTGCCTCGGAGGGACTGCGAGAAGGCCGAGCCCCGGGCGCCGGCGGCTCGGG
AACATGGCGGCGTGCATCGGGGAGAGGATCGAGGACTTTAAGGTTGGAAATCTACTCGGTAAAGGATCAT
TTGCTGGTGTCTACAGAGCTGAGTCCATACACACTGGTTTGGAAGTTGCAATCAAAATGATAGATAAGAA
AGCCATGTACAAAGCTGGAATGGTACAGAGAGTCCAAAATGAGGTGAAAATACATTGCCAGTTGAAACAC
CCCTCTGTCTTGGAGCTCTATAATTACTTTGAAGATAACAATTATGTCTACCTGGTATTGGAAATGTGCC
ACAATGGAGAAATGAACAGATATCTGAAGAACAGAATGAAGCCTTTCTCAGAAAGGGAAGCTAGGCACTT
CATGCACCAGATTATCACAGGAATGTTATATCTTCATTCTCATGGCATATTGCACCGGGACCTCACACTC
TCTAACATCTTACTTACGCGGAATATGAACATAAAAATTGCTGACTTTGGACTAGCAACGCAGTTGAATA
TGCCACATGAAAAGCACTATACACTCTGTGGGACTCCTAATTATATTTCACCAGAAATTGCAACTCGAAG
TGCACATGGACTTGAATCTGATATTTGGTCATTGGGCTGTATGTTTTATACGTTACTTATTGGAAGACCA
CCTTTTGACACTGACACAGTCAAGAACACATTGAACAAAGTAGTCCTGGCAGATTATGAAATGCCAGCCT
TTTTGTCACGAGAGGCCCAGGACCTTATCCACCAGTTACTTCGTAGAAACCCTGCAGATCGGTTAAGTCT
GTCTTCTGTGTTGGACCATCCTTTCATGTCACGAAATCCTTCACCAAAGAGTAAAGACGTAGGGACTGTA
GAGGACTCAATGGATAGTGGGCATGCTACACTTTCCACAACAATTACAGCCTCTTCTGGTACCAGTTTGA
GTGGCAGCCTACTTGACAGAAGACTTTTGGTTGGTCAACCACTTCCAAATAAAATTACTGTATTTCAAAA
AAATAAAAATTCAAGTGACTTTTCTTCAGGAGATGGAAGTAATTTTTGTACTCAATGGGGAAATCCAGAA
CAAGAAGCTAATAGTAGGGGACGGGGGAGAGTGATTGAAGATGCAGAAGAGAGGCCGCATTCTCGATACC
TGCGCAGAGCTCATTCCTCTGATAGAGCCAGCCCCTCTAATCAGTCTCGAGCCAAAACATACTCAGTAGA
ACGTTGTCACTCAGTAGAAATGCTTTCAAAGCCTAGAAGATCACTGGATGAAAATCAACACAGTTCCAAT
CATCATTGTCTAGGAAAAACTCCTTTTCCATTTGCAGACCAGACACCTCAGATGGAAATGGTACAGCAGT
GGTTTGGGAATCTGCAAATGAATGCTCATTTAGGAGAAACTAATGAGCACCACACCGTTAGCCCAAACAG
AGATTTCCAGGACTATCCAGATTTGCAGGACACGTTACGAAACGCTTGGACTGACACGAGAGCCAGCAAG
AATGCTGATACTTCTGCCAATGTTCATGCTGTAAAGCAGCTGAGTGCCATGAAATACATGAGTGCACATC
ACCATAAGCCTGAGGTCATGCCACAGGAGCCGGACCTACATCCTCATTCTGAACAAAGCAAGAATAGAAG
TATGGAGTCGACACTGGGTTACCAGAAACCTACCTTAAGAAGTATTACATCTCCTCTGATTGCTCACAGA
TTAAAGCCAATCAGACAGAAAACCAAAAAGGCTGTGGTGAGCATCCTTGATTCAGAGGAGGTGTGTGTGG
AGCTTCTGAGAGAGTGTGAGTCTGAAGGATATGTGAAAGAAGTGCTTCAGATATCGAGTGATGGGACTAT
GATCACTGTTTATTACCCGAACGATGGAAGAGGCTTTCCTCTTGCTGACAGACCTCCCTTGCCTACTAAC
AACATCAGTAGGTACAGCTTTGACAATCTACCAGAAAAATACTGGCGGAAATATCAGTATGCTTCCAGAT
TCATTCAGCTAGTAAGATCTAAAACTCCCAAAATCACTTATTTTACAAGATATGCTAAATGTATTTTGAT
GGAAAATTCTCCTGGTGCTGATTTCGAAGTTTGGTTTTATGATGGAGCCAAAATACATAAAACTGAAAAT
TTAATTCACATAATTGAGAAAACAGGGATATCTTATAATTTAAAAAATGAAAATGAAGTTACCAGCCTGA
AAGAGGAAGTAAAAGTATATATGGACCATGCTAATGAGGGTCACCGTATTTGCTTGTCACTGGAATCTGT
AATCTCTGAGGAGGAAAAGAGAAGCAGGGGTTCTTCATTCTTCCCTATAATCGTAGGAAGAAAACCTGGT
AATACTAGTTCACCTAAAGCCTTATCAGCTCCTCCTGTGGACCCAAGCTGCTGTAAGGGAGAGCAGGCGT
CAGCAAGCAGACTGAGCGTGAATAGTGCCGCTTTCCCCACACAGTCCCCAGGACTCAGTCCTTCCACTGT
GACAGTTGAAGGACTTGGCCACACAGCGACTGCCACAGGAACAGGCGTCTCTTCAAGTCTTCCTAAATCT
GCACAGCTTTTGAAATCTGTTTTTGTGAAAAATGTTGGTTGGGCTACACAGCTAACTAGCGGAGCTGTGT
GGGTTCAGTTTAATGATGGGTCACAGTTGGTTGTCCAGGCAGGAGTATCTTCCATCAGTTACACATCACC
AGATGGTCAGACAACTAGGTATGGAGAAAATGAAAAATTACCTGAATACATCAAACAGAAATTACAGTGT
CTTTCTTCCATCCTTCTGATGTTTTCTAATCCAACTCCTAATTTTCAGTAATTTAAGTCTCAGAAGTCTA
TATTTAATAAATGACTTTTTGGCTGGCTTTCAAGTAAGTGATTTTTTAAATTTACTTTAACTTCAGAAAG
CCTTTCTATTAAACAGAATTTTAATATACACAATAAAAATATAATAAGAAAACAATAAAATTTCAGTTAC
CTAATATAGTGGTCATAAGGCTAGGACATCTAATTTTGCTCCAAGCATGTAATCCTTCAAAGTTTGTGCT
CCTATGTTTGTATTGAACTAAGTTGTGTATGGCTTGTTTGTTTTTGTTATTTTCTTTACTAATAAGACAT
TGAGAATCACGGACAAAACATAGTTTTCAATTTTTGAATGTGTAAATAATGTATTATAAGCAATATGTAA
ATGTGTATATTTTATATTTATTTTTATAGCACTTGTGTCTGATAAGATTTCTGCAAATACATTTTATAAA
ATAAACACAGTGGTAAGTTTTCCTTAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5150173 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5150173 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14263 same embryo
 EMAGE:14266 same embryo
 EMAGE:14264 same embryo
 EMAGE:14268 same embryo
 EMAGE:14265 same embryo
 EurExpress:euxassay_003979 same experiment
 MGI:4827275 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS