EMAGE mouse gene expression spatial database

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EMAGE:14311

Pi4k2b phosphatidylinositol 4-kinase type 2 beta ( MGI:1914323)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004143. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004143_01	euxassay_004143_02	euxassay_004143_03	euxassay_004143_04

euxassay_004143_05	euxassay_004143_06	euxassay_004143_07	euxassay_004143_08	euxassay_004143_09

euxassay_004143_10	euxassay_004143_11	euxassay_004143_12	euxassay_004143_13	euxassay_004143_14

euxassay_004143_15	euxassay_004143_16	euxassay_004143_17	euxassay_004143_18	euxassay_004143_19

euxassay_004143_20	euxassay_004143_21	euxassay_004143_22	euxassay_004143_23	euxassay_004143_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14311_wholemount_strong.wlz

14311_wholemount_moderate.wlz

14311_wholemount_weak.wlz

14311_wholemount_possible.wlz

14311_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14311_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7836
Entity Detected:	Pi4k2b, phosphatidylinositol 4-kinase type 2 beta ( MGI:1914323)
Sequence:	sense strand is shown >T7836 GTCCCAGTCCGGAGCCCGGCGGCTCTGAGCGCCGGAGCCGCCCCGGGACATCATGGCGGAGGCCTGCGAG CCCACCCGCCCCTCGGAGGACGAGGACGAGGAGCGGGAGCCGCTGCTGCCTCGCGTTGCCTGGGCCCAGC CGCGGAGGGTCGCGCCCGGGAGCGCCGTGAGAATGCAGGCGGACGAGGGCGCGGATGTCCTCCGCGAGCC CGCTACCGACGAGCCGCCGGCGGTGTCCGGGGAAGGGTCGATCTCCGCGAGCTTGTCCACCGAGCTGGAT CGGACCCGCACTACGAGCTCAGAAACAAATACATTCTTGGAAGATCCAGAATTTGCTGATATTGTATTGA AAGCAGAGCAAGCAATAGAAATTGGAGTTTTCCCAGAAAGAATTTCTCAAGGGTCAAGTGGAAGTTACTT TGTAAAGGATTCTAAAAGGAATATTATTGGCGTGTTTAAACCCAAATCAGAAGAGCCTTATGGCCAGCTG AACCCCAAGTGGACCAAGTACGTCCATAAGGTTTGCTGTCCCTGCTGCTTTGGCCGAGGCTGCCTGCTTC CTAACCAGGGGTACCTTTCTGAAGCTGGTGCCTACCTGGTGGATGTCAAACTTAATCTGGGTATTGTGCC TAAAACAAAGGTGGTTTGGCTTGTCAGTGAGACATTTAACTACAGTGCAATTGACCGTGCAAAATCAAGA GGCAAAAAGTATGCCTTAGAGAAAGTGCCAAAAGTAGGCAGAAAGTTCCATCGGATAGGACTTCCTCCTA AGGTTGGTTCCTTTCAGCTGTTTGTGAAAGATTACAAGGAGGCTGAGTATTGGCTTAGGAGGTTTGAAGC GGAGCCTCTGCCCGAGAACATTAGAAAACAGTTTCAGTCACAGTTTGAAAAATTAGTGATTCTGGATTAC ATCATCAGGAACACAGACAGGGGAAATGATAACTGGCTAGTCAAATATGATGAGATGAAGTATGCAAAGA AAATTGAAAGTGAGGAATCAAACTGGATTGATAATAAACAATTGCTTATTAAAATAGCTGCAATTGATAA TGGGCTAGCATTTCCCTTTAAGCATCCTGATGAATGGCGAGCATATCCATTTCACTGGGCTTGGCTTCCT CAAGCAAAGGTTCCTTTTTCTGAAGAGACTCGAAACTTGATTCTACCCTATATTTCTGACATGAACTTTG TACAAGACTTGTGTGAAGATCTTTATGAACTTTTTAAGACTGACAAAGGATTTGACAGAGCTGCTTTTGA AAATCAGATGTCTGTGATGAGAGGCCAGATCTTAAACCTCACTCAGGCACTGAGAGACGGGAAGAGTCCT ATGCAGCTGGCACAGATGCCATGTGTGATCGTGGAGTGCAGCAAAAGTGGCAGCCAGGGCCGTGTGGTAC ACCTGGGCAGCTCCTTCACCCAGACGGTCCACTGCAGGAAACCGTTCTTCTCCTCCTGGTAGCAGATGTA AGGGGAACAACTGTGTGGTGTTACTGTGCTGATAAAATATTACAGTGATGTAAATCTGCTGCTGAGAGCA CCAGTCGCCAGAACCTCAAATAATTCAAGTCTTTAAATATATAGAAAATAATACTATATTTTGAATGTAA TCAAAAGTTTACGATTTTTTGTCCAAATATTCTATTTCAGGGAAGAAGTGCTATTATCTATATTGTATTT TTATAGAAACATTGTATTTTATGTTGTTATTACTTTAAAAAGTAACTTCATGGTTTACACATGCTAGCAT GACTGTAGTTCTAGAGAATTCCAGCTAGAATTCCTGTATTGACTTTGAATACAGGAACATTTTGTATACT TTTTAAACACGAGAAGAATCTTCTTGCATTCATTCCAAAAGACTTGAGGATGGCCAAGCCAGAAACTGCT CTTGTAGACTCCCAACAGAGGACCCAGTGGGTAGAGCGGACAGTGATAGTAAAGCAACTATACCTTCAGT TTAAAAGCTGCTGTTTTAAAACTGGAATCAAGTGCCCAAAGGTGGTCATTACCGGGAACCTGAAAGTTAA GTTCTGCCTAAGTGACAGATGACTATAGTAAGTTTTGAATTTTGATATATTTGTTGATGAATTATTCTGT TTTAGTGAAGGGGGAAAAAATCTAAAGACATCTCCCCTCCTTTTTGAAAACAAGAAATAACTGAAAATTG AATGGAATAATATGAAATTAAGGTAGATGTCCAGTATTTTAAAATTACAATATTGCCCAAAAAAAATTCT TGGTTTTTTTTTTTTTCTCATATGGGATCAAATAATTTAAAATTAGTACTTCAAAACACTAATCTTAATC AGTTCTGTACTCTAGTCAGTATGGAACATCACTTCAAAGCAAGCCATTCCCTGAAGCTTATACTGAAGTC AGGAACACGGTGCTCAGACACGCCTGGGGCGACTGGAAGCTGATCTCACCATTCACTTTAGGTTGCTGTC TTAGGGGTTTTTTAATGAGCTATTTTTGTATGAGAAGGTAGTTACATCCAGGTGGTTAAATTAAGGTGTG GTAGTCACCGTCTTGGTGCAGGTAAGGAGTGAGATACCACTGCCCTGAGGAGTCAGTTGGAAACTCGTCC TCCTACCTCTTCTGACCCACCTCCCATTAGCTGGGGTTTGAGATAATGGCAACCTGACTGGAGTATCAAA CTATACCACCAGCTGTTGGTAAATACTAGTCATTGATTTGTGCCTTTCTTCTGCAGTAGAGCGCATACTT GCCTCTGCTGTGGTACGGCTTTCACAAAGGATTTTCTTTTCATTGTTTTGAAAATTGCTATATTAATTTT TATAACAGAGAACCTAGTAAATTGCTACTGTTTATAGTCTAAAATTGAAATAGTTAAAGACTATTTTAAA CATCTGACCATGTTAGAATAGTTGGGGTATTTAAAAATTACAAGTAAAAGTACTTACGCTGTACTTTGTA AGCATTCATGAACTTAATTCTTATTTAAATAGTGAGGAGTTTTTTAATGAGCATATTTATATTGTCAAAT ATGATAAACAAAAGGAATAAAAATCATTGTCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6335661 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6335661 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14314 same embryo
	EMAGE:14310 same embryo
	EMAGE:14313 same embryo
	EMAGE:14312 same embryo
	EurExpress:euxassay_004143 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source