EMAGE mouse gene expression spatial database

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EMAGE:14314

AU019823 expressed sequence AU019823 ( MGI:2143205)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004127. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004127_01	euxassay_004127_02	euxassay_004127_03	euxassay_004127_04

euxassay_004127_05	euxassay_004127_06	euxassay_004127_07	euxassay_004127_08	euxassay_004127_09

euxassay_004127_10	euxassay_004127_11	euxassay_004127_12	euxassay_004127_13	euxassay_004127_14

euxassay_004127_15	euxassay_004127_16	euxassay_004127_17	euxassay_004127_18	euxassay_004127_19

euxassay_004127_20	euxassay_004127_21	euxassay_004127_22	euxassay_004127_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14314_wholemount_strong.wlz

14314_wholemount_moderate.wlz

14314_wholemount_weak.wlz

14314_wholemount_possible.wlz

14314_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14314_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7835
Entity Detected:	AU019823, expressed sequence AU019823 ( MGI:2143205)
Sequence:	sense strand is shown >T7835 CGCGAGGCTGCGGCGCACGATTAAAGAATGTCCCGGGTTCCGTTGGGGAAAGTCCTCTTGAGGAATGTCA TCCGGCACACAGATGCTCACAATAAGATTCAGGAGGAGTCTGACATGTGGAAAATCAGAGAACTAGAGAA GCAGATGGAGGATGCTTACCAGGGGACCAGAAGGAACACAGTGCCCAGCAGCTCAAGTCGGATGAGAAGT GATGGTTTTGATGAAGAAAGTCAGAGAGATTACTGGAGGTCAAAGAATGAAATTTCTGGGGCCTTGGAAG ATGATTTTCTTAAGACTAAGTCCTGGAACAAGAAGTTATATGATTATGAATCCAATATACCAGACAGATG GGGTCACAGTGGTTATAAAGAGTTGTATCCTGAAGAGTTTGAAACAGACAGTAGTGATCAGCAAGACATT ACCAATGGAAAAAAACCATCTCCCCAGGTAAAATCATCTGCCCGTGAATCTCGAAAACATAAGAAGTCAA AGAAATCCCATAAGAAGAAGCAGAAAAAACGGTCACACAAAAAACAGAAGAAAAACAAAAAGGAAGTTAC GGACATAACAGCAGATTCCTCAAGTGAGTTCTCAGAGGAAACTCGAGCTTCTAGTACTAGGAAAAGGAAG CAACCACACAAGAGCAAGAAAAAGTCCAGGAAAAAGTCTCCTAAAATCACTTTCCCTTTAGGGGGAGAAA GTGGTACTTCTCAGTCAGAGGATTCTGCATCTAGCAGTTCTGAGGACATCGAGGAAAGAGATACTAAGAA AACCAAACGGAAAAAGAAAGAGAAGAGTGTCCATGTCCCCATGCCTGACCCTGAGGTACAGGAGAGGACA AGCAAGCGCAGGAACTGGAAGGTGGCTACCGACGCCAGGTCTGCTGAGAGCTCGGAGGATGACTAAGTGG GAGAGCAGCAGTCCTGCTTCCCACTGACTCGTTAGGGGGGTTGCCAGTGACTCCTGCAGCACAGGCAGGC GTCTGAGGTCAGAGTTGTCCTGCCCCAGATACCTTTTTTCCTATTTTGGCTTGTTAATCTCCATCCTCTT TTGTTAATAGGGAATCTGGTATTTTATTAAGGTTTCTTGAAAAAATTATTTTTTTCAATTAATCACTTAG GGTAGAATACCTATACAGCAAATTAAAGAACCCAGAAAGCTGAACTCAGTAATGACCTGGGCACACCAAT TGCAATGTTGGATTTGATGGAATCAAGAGTCCAGGATGGAGGAGGAAGACTGAAGTAGTGCTAAATAGAA TGGTTTGACAAGGAGACCGTGCTATTCATACAGGAGGCATGCTGCCTTCAGCTTTGGTGCTTTGGTTCTT CTGCAGTTGGGCCCTATAGGCATCCAGTTTACTGAGCGAGGAAATAGTTAAAAGAACAAACCTTTTCCCT TCATGGTAATCTCTACAGATGACTGCTAAGGAACCTAGGGTTTTCAGCCTAATCTCCCTGCTGGCCAGGG TCTACAGCTACATTTAGCATAACCCAAACATAACAGTAACTGGATAGTAAATGTTTTTCTAGTTCCATTG CTGTATACTGCCTAAATGGGCTTGTATTTGAAATTGTTTGGTTGTCCTATCTATCAAATGGAAAGAATTA GGAAGTAGTTATGTTGCCATGATGCTTTCAAAGAGATTCAGGGCATCAGCCATCTAAATGTCTGTTTTTT AAAAAAATTTTATTATTATTATTATTATTATTATTATTTTTGCTGAGTTGGGGATTGAACCGAAGGCCTT GCATATACTAGGCAAGTGCTCTACCATTGAGCCATACCCCCAGTCCTGTTGTTTTCCTTGTTCATTTCTA ATTCTATTAATGGATTTTAGATTTTCCCTGAAACCAGTTGAATCAATTCCAAAATGAAACACACTTCTCA GGGACATACCCACTTCCCAGTCTGACTTTGTGTTTTGATTATCAAGAACATATTATTTCCCCCTGCTATA TACTGTGATCCTGATATTAAAAGAAACCAAAATACCACTGGAAAGTGGCTGGTAGAACAGTTGGCTTTTC ATTAAGAGGAAAGATAAAGTGACATTTATCAGGGTTTTTGTTTTTGAGGTTGTCACTTTGGTACTGAGCG AATGCATGAAAACTCAAGACTGTGGGCCATGGTTCTGCCACTAATTGATTGAGTTCTAGAGCAGGTTAAC CTCCCTGGCCTTACTCTTTGCATGTGTAATACAAAGATACTTCATGGTTAGCATGACATACACCAGCAAT AGAATATGATAATGCTGACATCCCATGAAGTGGCATTTTATAGTAATAACTAAATTTGTGCTCTTTATGG AACATATTTCTAATAAAATGCTTGTTCTTAAAATATATGGTTGGCCAGATGTGCCCTATCCCTTGATTAT GAGCAGGTGCCGCCTTTTGGGGTACTGAAACACTAATGATTTTAGTACTCAAATGCCAATATTCCATGAG CCTTTTGGGGTACTGAAACACTAATGATTTTAGTACTCAAATGCCAATATTCCATGATATATTCTTTTCC TTGGGTCTAGTGGAGGGAGAGTCTAGTTACTACCTCATAACCTTCTAGAAGCAAGTGATATCTTTGTTGG AAGTAGTTTTGGGCATGCATTCTTAATGCAGTTTTGTGCTACAAATGTACTGATTCTAAGCACAGACTCG GGAAGATTGTCCTGCAGAATAAGGCACTTCCAAGTTTGAGTTTTGACCTTACCTTCCTCCCATACAACAG ACATATCTTTTCTCCAGGGAAAGCACATTTTGCCTGATTTAACACCCCTGCCCCACCACCCCGCACTGTT CTATCTTTTCTTGGTCACATAAGCAATAAAATTGTTTCTGTTCTAATCCATGCCTTAGGCTAAGGCTTTG ATGGTTCCTCTAATCCCCTTAACAGTGGTTTAGGACTACTTTTGAAAACCTGTCTCTCCCATTTCCACGA TCAGCTCTGGAACATGTATTAGTGTTCCTGAGAGATGATTATGTTGAATCCTAAATTGGGATTTAGAAGA ACAAGAATTGGAGACTTCACATTTATTAACTGGTGTGATACAAGTTGGAATTACTGAATTCCCATAAATA AAATTTCACTTTTGAACAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6335744 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6335744 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14310 same embryo
	EMAGE:14313 same embryo
	EMAGE:14311 same embryo
	EMAGE:14312 same embryo
	EurExpress:euxassay_004127 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source