EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14317

Elac2 elaC homolog 2 (E. coli) ( MGI:1890496)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004154. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004154_01	euxassay_004154_02	euxassay_004154_03	euxassay_004154_04

euxassay_004154_05	euxassay_004154_06	euxassay_004154_07	euxassay_004154_08	euxassay_004154_09

euxassay_004154_10	euxassay_004154_11	euxassay_004154_12	euxassay_004154_13	euxassay_004154_14

euxassay_004154_15	euxassay_004154_16	euxassay_004154_17	euxassay_004154_18	euxassay_004154_19

euxassay_004154_20	euxassay_004154_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14317_wholemount_strong.wlz

14317_wholemount_moderate.wlz

14317_wholemount_weak.wlz

14317_wholemount_possible.wlz

14317_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14317_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5631
Entity Detected:	Elac2, elaC homolog 2 (E. coli) ( MGI:1890496)
Sequence:	sense strand is shown >T5631 GGTCGGGTGCATGTGGGCGCTCCGCTCACTGTTGCGTCCCCTTGGCCTGCGCACCATGTCGCAGGGTTCG GCTCGTCGGCCGCGGCCATCCAAAGACCCACTGCGACACCTGCGTACGCGGGAGAAGCGCGGCCCGGGTC CCGGGGGCCCGAACACCGTGTACCTGCAGGTGGTGGCGGCGGGCGGCCGGGACGCGGGGGCTGCTCTCTA TGTCTTCTCGGAATACAACAGGTACCTTTTTAACTGCGGAGAAGGCGTCCAACGACTTATGCAGGAACAC AAACTGAAAGTCGCTCGCTTGGACAACATCTTTCTGACTCGGATGCATTGGTCAAATGTTGGGGGGTTGT GTGGAATGATTTTAACTTTAAAGGAAACCGGGCTTCCCAAATGTGTTCTGTCTGGACCACCACAGCTGGA GAAATATCTAGAAGCAATCAAAATATTTTCTGGTCCATTGAAAGGAATAGAACTGGCCGTGCGGCCTCAC TCTGCACCAGAATACAAGGATGAGACCATGACTGTTTACCAGGTCCCTATCCACAGTGAACGGAGGTGTG GAAAGCAACAGCCATCCCAGAGCCCCAGAACATCTCCCAACAGGCTCAGTCCCAAACAGTCATCGGACTC TGGATCAGCTGAAAATGGGCAGTGCCCTCCAGAGGACAGCAGTGCAGGTGCCAACAGGAAAGCATGGGGC AGGGACCCCTCCTTAGTGGTAGCTTTTGTCTGCAAGCTTCACTTGAGGAAAGGAAACTTCTTGGTGCTTA AAGCAAAGGAGCTGGGCCTTCCTGTTGGGACGGCCGCCATTGCACCCATCATTGCTGCTGTCAAGGACGG GAAGAGTATCACTTACGAAGGAAGAGAGATTGCTGCTGAAGAGCTTTGTACACCCCCAGATCCTGGTCTT GTATTCATTGTGGTAGAGTGTCCTGATGAAGGATTCATCCTGCCCATCTGTGAGAACGACACCTTTAAAA GGTACCAGGCAGAGGCTGATGCACCTGTGGCGCTGGTGGTCCACATAGCCCCAGAATCTGTACTCATCGA CAGCAGATACCAGCAGTGGATGGAGAGGTTCGGGCCTGACACACAGCACCTGATTCTGAATGAGAATTGC CCCTCGGTCCACAACCTGCGCAGCCACAAGATTCAGACCCAGCTCAGCCTCATCCACCCTGACATCTTCC CCCAGCTTACCAGCTTCTATAGTAAGGAGGAAGGGTCCACCCTCAGCGTGCCAACAGTTCGGGGTGAATG CCTCCTCAAGTATCAGCTCCGCCCCAAGAGAGAGTGGCAGAGGGATACCACACTCGACTGCAATACTGAT GAATTCATAGCTGAGGCCTTGGAGCTCCCCAGTTTCCAGGAGAGTGTGGAGGAGTATCGGAAGAACGTGC AGGAAAACCCAGCCCCAGCAGAGAAAAGAAGCCAGTATCCTGAAATTGTCTTCCTGGGTACGGGGTCTGC CATCCCAATGAAGATCCGAAATGTCAGTTCCACACTCGTCAACCTAAGCCCTGACAAGTCAGTGCTCCTG GATTGTGGAGAAGGCACTTTTGGGCAGTTGTGCCGTCATTACGGACAGCAAATAGACCGAGTCTTATGCA GCCTCACGGCTGTGTTTGTGTCCCACCTGCACGCCGACCACCACACGGGCTTGCTGAATATCTTGCTGCA GAGAGAGCATGCGTTGGCATCTCTGGGGAAACCCTTCCAGCCCTTGCTTGTGGTGGCTCCTACCCAGCTC AGGGCCTGGCTGCAGCAGTATCACAACCACTGCCAGGAGATTCTGCACCACGTCAGTATGATTCCTGCCA AATGCCTTCAGAAAGGGGCAGAGGTCTCCAATACTACATTGGAAAGGCTGATAAGCTTGCTGTTGGAAAC ATGTGACTTAGAAGAATTTCAGACCTGCCTGGTACGGCACTGCAAGCATGCTTTTGGCTGTGCACTGGTA CATTCATCTGGCTGGAAAGTCGTCTACTCGGGGGATACCATGCCCTGTGAGGCTCTGGTCCAGATGGGGA AAGATGCCACCCTCCTGATACATGAAGCCACTCTGGAGGATGGCTTGGAAGAGGAAGCAGTAGAGAAGAC ACACAGCACCACCTCCCAGGCTATTAATGTGGGGATGCGGATGAATGCGGAGTTCATCATGCTGAACCAC TTCAGTCAGCGGTACGCCAAGATCCCCCTTTTCAGCCCTGACTTCAACGAGAAAGTTGGCATCGCCTTTG ACCACATGAAGGTCTGCTTTGGAGACTTCCCGACAGTGCCCAAGCTGATTCCCCCACTGAAGGCCCTGTT TGCAGGTGACATTGAAGAGATGGTGGAACGCAGGGAGAAGAGGGAGCTACGGCTGGTGCGAGCAGCCCTC CTGACCCAGCAGGCAGACAGCCCAGAGGACAGAGAACCCCAACAGAAGCGGGCCCACACAGATGAACCAC ACAGCCCACAGAGCAAGAAGGAGAGCGTGGCAAACACTTTAGGAGCGCGAGTGTGACACGGCGAGGACTT GGCCTGCCTTCCCTGGCGAGGACTTGGCCTGCCTTCCCTGGCGAGGACTTGGCCTGCCTTCCCTGGCCTG GCGGCGCTCTGCTCACTTCCTCATGAGGCAGCACAGGATGTTGTGACTGCCCCGCAGCCTTCACCGAAAC AAGACCATGTGCCAAGCTCAGGGTTAATAAATCTATTTTAATAACATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6493124 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6493124 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14316 same embryo
	EMAGE:14315 same embryo
	EMAGE:14318 same embryo
	EurExpress:euxassay_004154 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source