EMAGE mouse gene expression spatial database

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EMAGE:14345

Slc33a1 solute carrier family 33 (acetyl-CoA transporter), member 1 ( MGI:1332247)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004169. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004169_01	euxassay_004169_02	euxassay_004169_03	euxassay_004169_04

euxassay_004169_05	euxassay_004169_06	euxassay_004169_07	euxassay_004169_08	euxassay_004169_09

euxassay_004169_10	euxassay_004169_11	euxassay_004169_12	euxassay_004169_13	euxassay_004169_14

euxassay_004169_15	euxassay_004169_16	euxassay_004169_17	euxassay_004169_18	euxassay_004169_19

euxassay_004169_20	euxassay_004169_21	euxassay_004169_22	euxassay_004169_23	euxassay_004169_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14345_wholemount_strong.wlz

14345_wholemount_moderate.wlz

14345_wholemount_weak.wlz

14345_wholemount_possible.wlz

14345_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14345_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5622
Entity Detected:	Slc33a1, solute carrier family 33 (acetyl-CoA transporter), member 1 ( MGI:1332247)
Sequence:	sense strand is shown >T5622 GGGGTTAGAATCGAGGGAATTAATGGTTCCTAGCAAACGTTGCTCGCGGTCGCCGCCACCGGAACTTGTT CTGATTCGCTGGCTCCGTCGGGAGGCACGGAACCCATTTTGGCAGGGAACCACTTCCCGAAGCGCGAGGC AGGAAGTTATCCTTTACCTCTGGTGTCTACTGAGGAGGGTGCCCGCTGCAGTGTCAGCTGGTGGACGGGC AAGAGAGCGTTCGAGTCCCCGCTGCAGTTAAGAGCCAGACTCCGTCGCAGCGCCACGGCACGCTCCGCCA GCCTCAAGCCGGGTGCGGCTGCACGGGGAGCTCTTGCCGGCTCCCTGAAGTCGCTCCTGTGGGCAGCGAC CCAGGCTTCGCCTGGGCCACCTGCCAGGGATCTGGCTCCCTGCTGCAAAACAGCGACCAGTCTCTCCGCG TCCGCCCTCCCGGCGTTGAACCACCGTGACCCGGGTCCAGAGAGACTGCCTTGGACCTGGGACTAGACTG ATCAAAACGCCAGCCTCAGGCCCCCACACACCGGGCTCTGCGTCGTCTCTGATATGTCACCGACCATCTC CCACAAGGACAGCAGTCGGCAGCGGCGGTCAGGCATGTTTAGCCACGCTCTGGATATGAAGAGTGGCCCG CTGCCACCGGGCGGCTGGGATGACAGTCGCCGAGACTCGGTGGGCGGGGAGGGGGACCGGGAAGTTCTTC TAGGTGATGCCGGCCCTGGTGACTTACCTAAAGCCCCGCGGAGCTATAGATCCGAACTAAGTAGCATTTT GTTACTACTCTTTCTTTACGTGCTTCAGGGCATTCCCTTAGGCCTGGCGGGGAGCATCCCCCTCATTTTG CAAAGCAAAAATGTTAGCTATACAGATCAGGCTTTCTTCAGTTTTGTCTTCTGGCCATTCAGTCTTAAGT TGCTCTGGGCTCCCTTGGTTGATGCCGTCTACTTTAAGAACTTTGGTCGTCGCAAGTCCTGGCTTGTCCC TACTCAGTATACACTAGGAATCTTCATGATATATTTATCCACTCAAGTGGACCGTTTGCTCGGAAATATT GATGGCAGAACACCGGATGTAGTTGCTCTCACTGTGACATTCTTCTTGTTTGAATTCCTGGCTGCCACAC AGGACATCGCTGTGGATGGTTGGGCATTAACTATGTTATCCCGGGAAAACGTGGGCTATGCTTCGACATG CAATTCTGTGGGCCAAACAGCTGGCTACTTTTTGGGCAATGTTTTATTTTTGGCTCTTGAATCTGCTGAC TTTTGTAACAAATACTTGCGGTTTCAGCCTCAACCCAGGGGAATCGTTACCCTTTCAGATTTTCTATTTT TCTGGGGAACTGTATTTTTAATAACAACAACATTAGTAGCCCTTCTGAAAAAAGAAAACAGAGAAGCGTC AATAGTAAAAGAAGAGACCCAAGGGATCACAGACACTTACAAGCTGCTATTCTCAATTATAAAAATGCCA GCAGTTCTGGCCTTTTGCCTTCTGATTCTAACGTCAAAGATTGGCTTCTCAGCAGCTGATGCTGTGACAG GCCTGAAGCTGGTGGAAGAAGGGGTGCCTAAAGAGCACCTGGCCTTACTAGCTGTCCCAATGGTCCCTCT GCAGATAATCCTGCCACTCCTCATCAGCAAGTATACTGCAGGTCCCCAGCCTCTGAACATATTTTACAAA GCCATGCCCTACAGATTATTGCTTGGATTAGAATATGCTCTACTTGTTTGGTGGACTCCTAAAGTAGAGC ATCAAGGAGGATTCCCTCTATATTACTATATTATAGTGCTGCTGAGTTATGCATTACATCAGGTCACTCT GTACAGCATGTATGTGTCGATAATGGCTTTCAATGCCAAGGTCAGTGATCCTCTTATTGGGGGAACATAC ATGACCCTTTTAAACACTGTGTCTAATCTGGGAGGAAACTGGCCTTCTACAGTGGCTCTTTGGCTAGTGG ATCCCCTCACAGTGAAGGAATGTGTAGGAGCTTCAAACCAGAATTGTCGGACACCTGATGCTATCGAACT TTGCAAAAAACTTGGAGGCTCGTGTGTTACAGCTCTGGATGGTTACTATGTGGAATCCATTATCTGTGTT CTCATTGGATTTGGTTGGTGGTTCTTTCTTGGTCCAAAATTTAAAAAGTTACAGGATGAAGGACCATCCT CATGGAAGTGCAAAAGGAACAACTGATGCCAACATTACTGGACATTCTAGAAGGTAACTGTAGCTTAGTG TTACCAAGAAAGCAATGATAATCAGTGTGTAGGAGTGTAAAATACCGTTTTAGGAAATTTTTAATTTAAA ATGCCAAATAGTTGAAAAGTACAGATGTATTTGTATGTTTGATTATATTTTCCTTTGCCTTATCAATGTA TTGAAAGTTTACTTAAGGCTAGGAAACAACCCTTTTGGTTTTATTACTTGATTTCTGTTAATTTACTGAC CAAAGCATATTTTAAGCTGCAGTGGAATAGTCATGGGTGGTCACCATGCTGCCAAGTATTGTTAGTGCCT GTCATTGAGTCGTATTTAACATGTTGGTACAATGTATCAAGTGGAGCAAAGCTTAATATGCAGGTACTCA CTAACTAGATTAACCTTATTGGAAGTTAATTCTCACTTTGAATTGTAAATTTGTAAAAATGTATGTGAAA TTTGATTGAAAGAAAATGTAGACTACTAAAACATTGAGTTGTTTTTCTGACTATAACCACATTGTACAAA TGAATCTGTTAAAAATAACTATTTTAAATGTACTTTCTGCTTTTGTAAGCATGAAAGATTTATAAAAAGA TTATCCAAACTATTTTTTTATAAAATGAGAGCTATGGTTTTTAATTTATTGATTTCCCTATCTCTCCCTC CCTTTGGGGGAAGCTATTTTGATTTCTTGTTAACTCTTGCCCTAGGTTTACATATGATTTTGTCACAAAT ACATCATGACACACAGACAACTAATGAGGGGATCAAGTTTGTGGTGTGTTTGTGATTATGACTGAGTTGT AAAAATAAAAATTTAATGTCATTCTTACTGTAGTTTTTTTTAATTCCCATGAGAAGAAAATGTAAACTTA TAATTAAATAAAATTTAAATGGGTACCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6492236 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6492236 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14348 same embryo
	EMAGE:14350 same embryo
	EMAGE:14347 same embryo
	EMAGE:14346 same embryo
	EMAGE:14349 same embryo
	EurExpress:euxassay_004169 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source