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EMAGE:14376

Psmb6 proteasome (prosome, macropain) subunit, beta type 6 ( MGI:104880)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14376 EMAGE:14376 EMAGE:14376 EMAGE:14376 EMAGE:14376
"Pseudo-wholemount" of euxassay_004312. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004312_01 euxassay_004312_02 euxassay_004312_03 euxassay_004312_04
EMAGE:14376 EMAGE:14376 EMAGE:14376 EMAGE:14376 EMAGE:14376
euxassay_004312_05 euxassay_004312_06 euxassay_004312_07 euxassay_004312_08 euxassay_004312_09
EMAGE:14376 EMAGE:14376 EMAGE:14376 EMAGE:14376 EMAGE:14376
euxassay_004312_10 euxassay_004312_11 euxassay_004312_12 euxassay_004312_13 euxassay_004312_14
EMAGE:14376 EMAGE:14376 EMAGE:14376 EMAGE:14376 EMAGE:14376
euxassay_004312_15 euxassay_004312_16 euxassay_004312_17 euxassay_004312_18 euxassay_004312_19
EMAGE:14376 EMAGE:14376 EMAGE:14376 EMAGE:14376
euxassay_004312_20 euxassay_004312_21 euxassay_004312_22 euxassay_004312_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14376Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14376_wholemount_strong.wlz
14376_wholemount_moderate.wlz
14376_wholemount_weak.wlz
14376_wholemount_possible.wlz
14376_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14376_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7205
Entity Detected:Psmb6, proteasome (prosome, macropain) subunit, beta type 6 ( MGI:104880)
Sequence:sense strand is shown

>T7205
CCACGCGTCCGCTTAGCTGTTCGTCGAGCGGGGTCTGCACCGGCCTTTGGGCCGGAGGCTCTCACCCCAG
ACTGGGAAAACCGGGAAGTCTCCACAGGGACCACGATCATGGCTGTGCAGTTTAATGGGGGCGTGGTTCT
AGGAGCGGACTCCAGGACAACCACTGGGTCCTACATCGCCAATCGAGTGACTGACAAGCTGACCCCTATC
CACGATCACATCTTCTGCTGCCGCTCAGGCTCAGCCGCTGATACCCAAGCAGTGGCAGACGCTGTCACTT
ACCAGCTTGGTTTCCACAGTATTGAACTGAACGAGCCTCCACTAGTCCACACAGCCGCCAGTCTCTTTAA
GGAGATGTGTTACCGGTACAGAGAAGATCTGATGGCAGGAATCATCATTGCAGGCTGGGACCCTCAAGAA
GGAGGGCAGGTGTACTCTGTTCCCATGGGGGGTATGATGGTAAGACAGTCCTTTGCCATCGGAGGCTCCC
GGAGCTCGTACATCTATGGCTATGTTGATGCTACGTATCGGGAAGGCATGACCAAGGACGAATGTCTGCA
GTTCACTGCCAATGCTCTCGCTTTGGCCATGGAACGCGACGGCTCCAGTGGAGGGGTGATCCGCTTGGCA
GCCATTCAGGAGTCAGGGGTAGAGCGGCAGGTGCTTTTGGGAGACCAAATCCCCAAGTTCACCATTGCCA
CGTTGCCACCTCCCTGAGGCCTGGTGTTTTAGGGAGTAGTAAAGGATGGTCCATACCGACGCAGAAGCTA
ATAAACAGTTTGTTAAAAGGAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4038758 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4038758 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14379 same embryo
 EMAGE:14381 same embryo
 EMAGE:14378 same embryo
 EMAGE:14380 same embryo
 EMAGE:14377 same embryo
 EurExpress:euxassay_004312 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS