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EMAGE:14379

Ercc1 excision repair cross-complementing rodent repair deficiency, complementation group 1 ( MGI:95412)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14379 EMAGE:14379 EMAGE:14379 EMAGE:14379 EMAGE:14379
"Pseudo-wholemount" of euxassay_004265. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004265_01 euxassay_004265_02 euxassay_004265_03 euxassay_004265_04
EMAGE:14379 EMAGE:14379 EMAGE:14379 EMAGE:14379 EMAGE:14379
euxassay_004265_05 euxassay_004265_06 euxassay_004265_07 euxassay_004265_08 euxassay_004265_09
EMAGE:14379 EMAGE:14379 EMAGE:14379 EMAGE:14379 EMAGE:14379
euxassay_004265_10 euxassay_004265_11 euxassay_004265_12 euxassay_004265_13 euxassay_004265_14
EMAGE:14379 EMAGE:14379 EMAGE:14379 EMAGE:14379 EMAGE:14379
euxassay_004265_15 euxassay_004265_16 euxassay_004265_17 euxassay_004265_18 euxassay_004265_19
EMAGE:14379 EMAGE:14379 EMAGE:14379 EMAGE:14379
euxassay_004265_20 euxassay_004265_21 euxassay_004265_22 euxassay_004265_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14379Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14379_wholemount_strong.wlz
14379_wholemount_moderate.wlz
14379_wholemount_weak.wlz
14379_wholemount_possible.wlz
14379_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14379_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7209
Entity Detected:Ercc1, excision repair cross-complementing rodent repair deficiency, complementation group 1 ( MGI:95412)
Sequence:sense strand is shown

>T7209
GAAGTGAGTCTAGCAGGAGTTGTGCTGGCTGTGCTGGCGTTGTGTCGCCTCTGTTTCCCCCCGTGGTATT
TCCTTCTAGGCATCGGGAAAGACCAGGCCCCAGATGGACCCTGGGAAGGACGAGGAAAGTCGGCCACAGC
CCTCAGGACCACCCACCAGGAGGAAGTTTGTTATCCCACTGGAGGAAGAAGAGGTGCCCTGTGCAGGGGT
CAAGCCCTTATTCAGATCGTCACGGAATCCCACCATCCCAGCAACCTCAGCCCACGTGGCCCCTCAGACG
TATGCTGAGTACGCCATCACCCAGCCTCCAGGAGGGGCTGGGGCCACAGTGCCCACAGGCTCTGAACCTG
CGGCAGGAGAGAACCCCAGCCAGACCCTGAAAACAGGAGCAAAGTCTAATAGCATCATCGTGAGCCCGAG
GCAGAGGGGCAACCCCGTGTTGAAGTTTGTGCGCAATGTGCCCTGGGAATTCGGTGAGGTGATTCCCGAT
TATGTGCTGGGCCAGAGCACCTGCGCCCTTTTCCTCAGCCTCCGCTACCACAACCTCCATCCAGACTACA
TCCATGAACGGCTGCAGAGCCTGGGGAAGAACTTCGCCCTTCGTGTGCTGCTGGTTCAAGTGGATGTGAA
AGATCCCCAGCAGGCTCTCAAGGAGCTGGCTAAGATGTGCATCTTGGCTGACTGCACCCTGGTCCTGGCC
TGGAGTGCAGAGGAAGCAGGGCGGTACCTGGAGACCTACAAGGCGTATGAGCAGAAGCCAGCCGACCTCC
TTATGGAAAAGCTGGAGCAGAACTTCCTATCACGGGCCACTGAGTGTCTGACCACCGTGAAATCTGTGAA
CAAGACCGACAGCCAGACCCTCCTGGCTACATTTGGATCCCTGGAACAGCTCTTCACCGCATCAAGGGAG
GATCTAGCCTTATGCCCGGGCCTGGGCCCACAGAAGGCCCGCAGGCTCTTTGAAGTACTACACGAACCCT
TCCTCAAAGTGCCTCGATGACCTGCTGCCACCTAGGCCCAGTGTCACAATAAAGAATTTTCCATGCCCAG
GCAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4039274 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4039274 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14381 same embryo
 EMAGE:14378 same embryo
 EMAGE:14380 same embryo
 EMAGE:14377 same embryo
 EMAGE:14376 same embryo
 EurExpress:euxassay_004265 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS