EMAGE mouse gene expression spatial database

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EMAGE:14445

9430015G10Rik RIKEN cDNA 9430015G10 gene ( MGI:2444364)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004261. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004261_01	euxassay_004261_02	euxassay_004261_03	euxassay_004261_04

euxassay_004261_05	euxassay_004261_06	euxassay_004261_07	euxassay_004261_08	euxassay_004261_09

euxassay_004261_10	euxassay_004261_11	euxassay_004261_12	euxassay_004261_13	euxassay_004261_14

euxassay_004261_15	euxassay_004261_16	euxassay_004261_17	euxassay_004261_18	euxassay_004261_19

euxassay_004261_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14445_wholemount_strong.wlz

14445_wholemount_moderate.wlz

14445_wholemount_weak.wlz

14445_wholemount_possible.wlz

14445_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14445_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6099
Entity Detected:	9430015G10Rik, RIKEN cDNA 9430015G10 gene ( MGI:2444364)
Sequence:	sense strand is shown >T6099 CCCACGCGTCCGGCGGCCGGTACGAAAGGAGCTCGGCTGGCGAGTGAATTCCTCTATCTAGGAAGCTCAG ACTACTATTGTTCCAGAATTGGAAATGCCCAGCTTGCCTGCTCCTTCAATGCTTCGGGGGCCGCTGCTAA GTAAAAAGTCTTCATAGAGATCTCCTTGTCAGCTCCCCAGCATGGCACTGCAGTGTCTCCTGCTCCTGAC TGGCCTTCTGACAGGAGGCGTGTGCAAGTCCACAGAAAGCCAGGCCCAGCAACCTGAGTGTTGCATGGAT GTGGTAGACTTCAATGCTACCTGCCTCGGTACAGGTCTCTGTGGCCCAGGTTGCTACAGGCACTGGAATG CAGATGGGAGCGCTAGCTGTGTCCGGTGCTGGAATGGGACCCTCCCGACATACAATGACTCTGAGTGTAG AATTCTCACTGGCCGGGGCATGCAGCTTCCCATGAACAGGAGCACGGGGACACCTGGACAGCCACATTTT GGGGGTCCTCATGTGGCAGCTTCTCTCTTCCTGGGGACACTCTTCATCAGCACAGGCCTCATCCTCTCTG TGGCTGGATTCTTCTACCTCAAGCGCTCCAGCAAACTTCCTGAGGTTTTCTACAGGAGAGACAGAGCCCC TGTCCTGCAGCCTGGTGAGACAGCTGCCATGGTCCCCCTGCCACAGTCTTCAGTGAGGAAGCCAAGATAC ATCCGGCGTGAGCAGCACCCAGACAAGAATAGGGACCCTTCTGCCTTCTCTACAGTGGAGGCCCACATCA GCAACGTCTGATCTAGAGGTCAACTGAGACTTCACAGTACAGCAGTGGAGACCCAAGGCCCCCTCCAGTG AGCTCACTACACAAAGGGCCTGGGCCTTAAGATATGCCAGAATGGACTCCCAATGGCAGACATGCACGAA TGCTAGGGGGATGCTAACGTCCATGCCATGAGATCGTCACTGTGTGGTTTAACAGGAGCAACCATCTGTG AGACTGAGGACTCAAGAGCAAGTCATGTGGGGTCTGTGCCCCTTAAGAGTAACAGCATCTATAGCAGCTA TAACACAGACCTGATCCAAACGGTCAAAACTTCTGGAGCCCACCCACTGGAGCCCATAGTAGACAGGACT CCATTCCCACCGAGCTAAGAAGATGAAACCAAGGTTCCAACAGTGGAGAAAACACAGCCTGGATACTGGT TCCAGAGATAGGTTGCTCTAGTGTCAGCCTGCTAGTTTAGCTGCTGACCTCTCAAGGCCCATGTTTCTCA GAGTTGCAGAATTGAGTATCCTTGGTTTTCTGATTCCAGAAGCCAGCAGAGCCTTGTCTTCCAGGGACAG ACTCCACCAGCTACCTTCAGGCTGGGCACTCAGAGCAAAATGTTTACCCGTGGGGCCTCCATCTCTGAAC ACTCAGGTCACTTGTCCCCTTGGGCTTGTGAGCTCATGCTGGTTAGCAGTCCTGTCTTTGCTCTCCCAAG ACTCCAGAAGCCTGTGAGCTGCTAGTGTGGCTGGTGGTGTTGGCCATGTGTTCCTATAGAAGGAGGTGGC CTGATGACCAGTGCCATCCTCCGTCTAGCATCTTCTTTGACGTCAAGCACACATCTGATGTTATGCACAG TGTTAGGAGTTTGCTTTCCCAGAGTTGAGCCAGTGACTCAGGTAGCAGCAGCAATTCCAAGCTGGACCAA TTCAATTAGACTAGATCTTCTCTCCTCAAGCTTTGTTGTCCCAAGGTCTCGTATTCAGTGTGGTGTGTGG GAGCGAACTACCCCATTGCCATGTCCAGGGGACAAGTAGGGAGGGGAAGAAGAAGCCCTCAGCCGGACTC AGAGCCTACTGTTGTCTGGTGGAACTGGCAGTGCAGCACAACCCATCCCCTCATCTTTCCCTGGCCATGT AGCCCCTCCCATTTCAGGACACCATCTTGCATGAAGAGGTGACCCCGCTCCTTAGGACTTGAGGTAGAAA GTGCCAGTCAAGCACGGCATGGTTTTCAGATAAGACTACATGCATCACTAGGCTTTGAGGGGGCCACTGG CAGTTTATGGATCTAATTGACCCTTGGGGAAAGCAGAACCCTCGACCTGGCCACCGGAGTTCATTTTAGT GGTCCCCCTCAAAGCCCATGTGTAGACGTACAGATGTGTGTCTGCTGACTAGAGGCAGAACCGGATAAAG AGAGCCAAATGAGAGCTTAGGGATTCTAGAAGTCCTGCCAGGTAGTTTGATAAGAATGCCTACCCAGTGC CCCCAGGCTTACATACTTGAATTGCTGAGTCATCAGGGATTACGGGAGAGGCTTAGGAGGTGTGGCTTTA TTGGAGGAAGTGTGTCTCTGGGGATGGGCATTGGGTCTCAAAAGCTCAAGCCAGGCCCAGTGGCTTTCAT TTCCTGCTGCCTGTGGATTTGGATGTAGAGCTCTCAGCTCCTCGTCCAGCACCATGTCCGCCTATGCTAC CATGCTTCCTGCCGTTCCGATAATGGACTAAACCTCTGAAACAGTAGACAAGCCCCAATTAAATACTTTG TTTTATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3493145 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3493145 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14447 same embryo
	EMAGE:14448 same embryo
	EMAGE:14444 same embryo
	EMAGE:14446 same embryo
	EMAGE:14443 same embryo
	EurExpress:euxassay_004261 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source