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EMAGE:14580

Fbxo36 F-box protein 36 ( MGI:1289192)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14580 EMAGE:14580 EMAGE:14580 EMAGE:14580 EMAGE:14580
"Pseudo-wholemount" of euxassay_005472. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005472_01 euxassay_005472_02 euxassay_005472_03 euxassay_005472_04
EMAGE:14580 EMAGE:14580 EMAGE:14580 EMAGE:14580 EMAGE:14580
euxassay_005472_05 euxassay_005472_06 euxassay_005472_07 euxassay_005472_08 euxassay_005472_09
EMAGE:14580 EMAGE:14580 EMAGE:14580 EMAGE:14580 EMAGE:14580
euxassay_005472_10 euxassay_005472_11 euxassay_005472_12 euxassay_005472_13 euxassay_005472_14
EMAGE:14580 EMAGE:14580 EMAGE:14580 EMAGE:14580 EMAGE:14580
euxassay_005472_15 euxassay_005472_16 euxassay_005472_17 euxassay_005472_18 euxassay_005472_19
EMAGE:14580 EMAGE:14580 EMAGE:14580
euxassay_005472_20 euxassay_005472_21 euxassay_005472_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14580Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14580_wholemount_strong.wlz
14580_wholemount_moderate.wlz
14580_wholemount_weak.wlz
14580_wholemount_possible.wlz
14580_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14580_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9821
Entity Detected:Fbxo36, F-box protein 36 ( MGI:1289192)
Sequence:sense strand is shown

>T9821
CGTCCCAAGATGGCGTCGTGGCTGCCGGAGACTCTGTTTGAAATTGTAGGACAAGGCCCAGCGCCGAGCA
AAGACTATTACCAGTTGTTGATCACCCGGACTCAGATCATCTTTAGATGGTGGAAGATTTCTCTGAGAAG
TGAGTATCGGTCAGCAAAACCTGGAGAAACAAAGGAAAGCCATGAAGACTTCCTAGATAACTCTCATCTC
CAAGTTCAAGTCGCCGTAGTATTTGGTACAAAAATATTAGACTATGTCTTCAACCTGTGCGAAGGTAAAT
TTGACTACCTGGAGCGGCTCTCCGACAGACTGCTGCTGAAGATCATCTGCTACCTGGACCTGGAAGATAT
TGCCAGCCTCTCCCAGACATCAAGCAAGTTTGAGAAGTTGTGCAAGAGTGACTTGCTGTGGGAACAGATA
GTCCAGTCCACCTGTGACACCATCACCCCTGACATGCGGGCCTTGGCGAAAAACATGGGCTGGAGGCAGA
TGTTCCTCACCAACAACATCCAGCTCCAGAGGCAGACCCGCAAGAAGAAGCAAAGACAGGAAAACCAGGC
AGAGAAGCTTGCTTAGGGGATGGCTGTTTCCAACAGCGGAGCCACAGTGGTCCGAATCTCCAGGTCCAAA
GCTCACTCTCTCTTAACAACAGGTTCTGCCGATTCCAGCATCACTTGAAAACCAGATTTTGCACACACAT
GCAAATCCACCTAGTGCCTTGCTCAAGCAACCCCCTCCTATTGCCCAACCAGGGCCCAAGTACTAAGGGC
TTCGAGCCAGGAAGTCTCTTAGACTACCACCTCCCTGATCCCCTTTTGCCCCACCCCCCAGGAAAGAGGC
ACTTGTATATGAACTAACAATAAAGAGCTATCAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6772363 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6772363 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14578 same embryo
 EMAGE:14583 same embryo
 EMAGE:14581 same embryo
 EMAGE:14579 same embryo
 EMAGE:14582 same embryo
 EurExpress:euxassay_005472 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS