EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14687

Naa16 N(alpha)-acetyltransferase 16, NatA auxiliary subunit ( MGI:1914147)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005707. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005707_01	euxassay_005707_02	euxassay_005707_03	euxassay_005707_04

euxassay_005707_05	euxassay_005707_06	euxassay_005707_07	euxassay_005707_08	euxassay_005707_09

euxassay_005707_10	euxassay_005707_11	euxassay_005707_12	euxassay_005707_13	euxassay_005707_14

euxassay_005707_15	euxassay_005707_16	euxassay_005707_17	euxassay_005707_18	euxassay_005707_19

euxassay_005707_20	euxassay_005707_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14687_wholemount_strong.wlz

14687_wholemount_moderate.wlz

14687_wholemount_weak.wlz

14687_wholemount_possible.wlz

14687_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14687_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8850
Entity Detected:	Naa16, N(alpha)-acetyltransferase 16, NatA auxiliary subunit ( MGI:1914147)
Sequence:	sense strand is shown >T8850 CGCGGCCCGGGTCCTGGCTGGTCGCTCGAGCAGGCCTCCCCATCGCCGCCACGATGCCCTGCGTGCAGCT GCCCGCCAAGGAGAGCGCCCTCTTCAAGCGCGTGCTGAAATGTTATGAACAGAAGCAGTACAAAAATGGC CTCAAGTTTTGCAAGATGATTCTTTCTAACCCGAAGTTTGCTGAACATGGAGAGACTTTGGCTATGAAAG GATTAATACTGAACTGTTTAGGAAAAAGAGAAGAAGCATATGAATTTGTTCGTAAAGGACTTCGCAGTGA CGTCAGGAGTCATGTCTGTTGGCATGTATACGGCCTCCTGCAACGTTCTGATAAGAAGTATGATGAAGCT ATTAAATGCTACCGAAATGCCCTCAAATTAGATAAAGATAATTTGCAAATCTTGAGGGATCTCTCTCTAT TGCAGATTCAGATGAGAGACCTTGAAGGTTATCGAGAGACGAGATACCAACTTCTTCAGTTGCGCCCAAC ACAGCGTGCTTCCTGGATTGGATATGCCATCGCTTATCATTTGCTGAAAGATTATGATACAGCGCTAAAG CTATTAGAAGAATTTAGACAAACGCAGCAAGTTCCTCCCAACAAAATAGCTTATGAATATAGTGAACTGT TACTATATCAGAATCAAGTGATGAGAGAGGCAAATTTATTTCAGGAATCTTTGGAACATATAGAAACATA TGAGAAGTTGATATGTGATAAACTTTTGGTGGAAGAAATTAAGGGGGAAATGCTGTTGAAATTGGGAAGA CTAAAAGAAGCCAGTGAAGTATTCAGAAACTTGATTGATTGGAATGCTGAAAATTGGTGTTATTATGAAG GCTTGGAAAAGGCATTGCAACTTCGTTCTCTAGACGAGAGGCTTCAGCTTTATGAGGAAGTCAGCAAGCA GCACCCCAGAGCAGTTTCACCCAGGAGGCTGCCGCTGAGTTTCGCCCCAGGTAAGAAGTTTCGAGAACTC ATGGATAAGTTCCTGAGACCAAACTTTAGCAAAGGGTGTCCACCTCTGTTCACTACTTTGAAATCCTTGT ATTGTGATACAGAAAAGGTTTCAATAATCCAGGAACTTGTTACTAATTATGAAGCCTCTCTTAAAATGAA TGGCTATTTTAGCCCTTATGAGAACGGGGAGAAGGAACCCCCAACAACTCTAATCTGGGTCCAGTATTTC CTGGCACAGCATTATGATAAACTTGGGCAGTATTTTCTGGCTTTGGAATATATTAATGCTGTAATTGCTA GTACTCCAACTCTAATAGAACTATTCTACATGAAAGCAAAAATTTACAAGCATATGGGTAATCTCAAAGA AGCCGCACAGTGGATGGATGAGGCACAGTCTTTGGACACGGCTGACAGGTTCATCAATTCCAAATGTGCC AAATACATGCTTCGAGCAAATATGATAAAAGAAGCAGAGGAAATGTGCTCCAGGTTCACAAGGGAAGGAA CATCTGCCATGGAGAATCTGAATGAAATGCAGTGTATGTGGTTTGAGACGGAGTGCATTTCTGCCTATCA GCGCCTGGGGAGATATGGGGATGCCTTGAAAAAGTGCCATGAAGTAGAGAGGCATTTTCTTGAGATAACC GATGATCAGTTTGACTTCCATACATACTGCATGAGAAAGATGACCCTCCGTGCTTATGTTGGCCTCTTGA GATTAGAAGATGCTCTCAGAAGACATACTTTTTATTTCAAGGCTGCTAGATCAGCAATTGAAATATATTT GAAATTACATGATAACCCTTTAACCAATGACAGCAAACAACAAGACATAGATTCAGAAAACCTGTCAGCC AAAGAAATGAAGAAAATGCTTAGCAAGCAAAGAAGAGCTCAGAAAAAGGCTAAGGTAGAAGAAGAGAGAA AGCACACAGAAAGGGAACGCCAACAGAAAAACCAAAAGAAAAAAAGAGAAGAAGAAGAAGAAGTTACCAG TGGTCATAAGGAAGAACTTATTCCTGAAAAACTAGAAAGGGTTGACAATCCATTAGAAGAAGCCATCAAG TTCCTCACTCCTCTGAAAACCCTTGCTGCTGAGAGCATTGATACCCACCTGCTGGCCTTTGAAATATATT TTAGAAAAGGGAAGTTTTTGCTAATGTTGCAGTCTGTTAAACGAGCCTTTGCCATTGAAAGTAATAATCC TTGGTTACATGAATGCTTGATTAAGTTTTCTAAATCTGTCTCTAATCACAGTAATCTTCCAGACATTGTG AGCAAAGTTCTAGCTCAAGAAATGAAGAAGATATTTGTCAACAAAGATTTGCACAGCTTTAATGAGGATT TCCTCAGACACAATGCTACCTCTCTTCAGCATCTACTTGCAGGTGCTAAAATGATGTATTTTCTGGACAA GTCAAGGCAAGAAAAAGCAATTGCTACAGCCACTAGGTTGGATGAAACTATAAAAAATAAAAATGTGAAG ACTCTAATAAAGGTGTCTGAGGCCCTCCTGGATGGCAGCTTTGGGAACTGTAGCTCCCAATATGAAGAGT ATAGGAAGACCTGCCATAGCCTGCTCCCACTCACACCTGCTTTCCTGCCTGCTGCGAGGGAAGCCCTCGG TCTCAGTGCTGAGCTGAACCACACAGCTGACCACAAGCTCTTAATGAATGAAATTTGAATCTTGTGGGAA GTTGCTCCTAAGACTCAAAGCTGAATTCACATCAGGTTTCTTTTTCACTGTACATTTTAAACTGGCGTAA AATAAAATATTTGGATAGAAGGGGTTTTTTGTTTGTTTGTTTTTTGTTTTGTTTTGTTTGGTTTGGTTTG GTTTTTTCGAGACAGGGTCTCTCTGTGTAGCTCTAGCTGTCCTGGAACTCACTATAGACCAGGCTGGCCT ACAGAAATCCACCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAGGTGTGAGCTACCACGCCCGGCTCTGG ATAGAAGTTTTAAATGGAATATTGTGTAAACTTATTTTATGCTGTGTCTGGCCTTCAATTTACATGGACA TCTGTTACAGGTACTTGTTTATACTTGTACAGTCTTATACTTCTGAATTATGAGTGTTGTTGCTTCTGTA TTATATGCTTCAGTTGCTGATTAAGGTATAGACAGCTTTCTGACACTATTTTCTTTAGGTCCTAGTTTCA GAATCTGTTTTGACCTCTGGTTTATTTGATTATTTTGAAGGGTTAACTGAATGTTAAGAAATAGCCATAT TTCTCATTTTGGTCGATAATGTGATTTGTCCCCTTTTTACCTTAACTTGTATGATTATTTCCTTTGTCAT AGCCATAAAAGTGATACTTTATGGTTGAATAAGGATACACCAGTGTTATGTTGATGATTCCGAATGTGAA GACTGGTTTGCCTCTTAGTCATGTAAATTTAACAGTTAAATTTGTTGTGTATGTAGTCTAGTCTTACAGG CGATTGGGTAGGAATTGTGTTTTCGTGGTTGAGCTGCTTGGAGACTTAGCAAGCAGCTACTCTGAGAGGT TTTCTTCAAGGGAGGAGGACTTGGCAGAGTACACACTAGAACACAGCACTACCTCCCTCACAGCCCTTCA GTGAAGTTTTAGATCTCTCAGATGAGACCTTAAACTACAATTAAACTATTAACTCATAGTGCTGTTGGAC AGTATTTGAGGGGAGCCCTTTTTGCCATATCTACATCATGGAATCACTCCATTTCTCAGTGTCAATATGT CTGTAAATCTACCTGATATAAACCCAGAGAGATGATTTTATTTAGACAGTTCTAATTAAAGTGGTTTTCC TTTATTTCTTAGATTCATAGCTATTGAAGAGGAAAAAAGATCACTGTAAAATAATGCCAACCTAGACAAT GCTACAATAAATTATTTCATGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6412745 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6412745 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14688 same embryo
	EMAGE:14689 same embryo
	EurExpress:euxassay_005707 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source