EMAGE mouse gene expression spatial database

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EMAGE:14688

Hadha hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit ( MGI:2135593)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005619. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005619_01	euxassay_005619_02	euxassay_005619_03	euxassay_005619_04

euxassay_005619_05	euxassay_005619_06	euxassay_005619_07	euxassay_005619_08	euxassay_005619_09

euxassay_005619_10	euxassay_005619_11	euxassay_005619_12	euxassay_005619_13	euxassay_005619_14

euxassay_005619_15	euxassay_005619_16	euxassay_005619_17	euxassay_005619_18	euxassay_005619_19

euxassay_005619_20	euxassay_005619_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14688_wholemount_strong.wlz

14688_wholemount_moderate.wlz

14688_wholemount_weak.wlz

14688_wholemount_possible.wlz

14688_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14688_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5550
Entity Detected:	Hadha, hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit ( MGI:2135593)
Sequence:	sense strand is shown >T5550 GAAGAGCTTTCGTCCTCTTCTGCTCAAGATGGTGGCGTCCCGGGCGATTGGCAGTCTCAGTCGCTTCTCT GCCTTCAGGATCCTACGCTCCAGAGGCTGCATTTGCCGCAGCTTTACAACGTCTTCTGCTCTGCTGACCA GAACCCATATTAATTATGGCGTCAAAGGGGATGTGGCAGTTATTCGGATTAACTCACCCAATTCAAAGGT AAATACATTGAATAAAGAAGTACAGTCAGAGTTCATAGAAGTCATGAACGAAATCTGGGCCAACGACCAA ATCAGGAGTGCTGTCCTTATCTCATCAAAGCCTGGCTGCTTTGTCGCAGGGGCTGACATCAACATGCTAT CCTCTTGTACAACCCCCCAAGAAGCAACACGAATATCACAGGAAGGTCAGAGAATGTTTGAGAAACTTGA AAAGTCCCCAAAGCCCGTTGTGGCCGCCATCAGTGGATCCTGCTTGGGAGGAGGACTTGAGCTTGCCATA GCATGCCAATACAGAATAGCAACAAAAGACAGAAAAACAGTATTAGGTGTCCCTGAAGTGTTGCTGGGGA TCTTACCAGGAGCCGGAGGTACCCAGAGACTGCCCAAAATGGTGGGTGTGCCTGCTGCATTTGACATGAT GCTGACTGGCAGGAACATTCGTGCAGACAGAGCAAAGAAAATGGGACTGGTTGACCAGTTGGTGGAACCG CTGGGACCAGGAATAAAATCACCAGAAGAAAGGACAATTGAATACCTAGAGGAAGTTGCAGTTAATTTTG CCAAAGGCCTGGCTGATCGGAAAGTCTCTGCAAAGCAGAGCAAAGGCCTTGTGGAAAAGCTGACAACGTA TGCCATGACTGTCCCATTTGTCAGACAACAGGTTTACAAAACAGTTGAAGAAAAAGTGAAGAAGCAGACT AAAGGCCTTTATCCTGCCCCTTTGAAAATAATTGATGCTGTGAAGGCTGGACTTGAGCAAGGCAGTGACG CTGGTTATCTTGCTGAATCGCAGAAATTTGGAGAGCTTGCATTGACCAAAGAATCGAAGGCCCTGATGGG GCTTTATAACGGCCAGGTCCTGTGCAAGAAAAATAAATTTGGAGCACCACAGAAGAACGTTCAGCAGCTA GCCATCCTTGGCGCAGGGCTGATGGGGGCTGGCATTGCCCAGGTCTCTGTGGATAAGGGACTGAAAACTC TTCTTAAAGACACCACAGTGACGGGGCTGGGCCGGGGACAGCAGCAAGTGTTCAAAGGGCTGAACGACAA AGTGAAGAAGAAAGCTCTCACATCATTTGAAAGGGATTCCATCTTCAGCAACTTGATTGGGCAGCTGGAT TACAAAGGCTTTGAGAAGGCCGACATGGTGATTGAGGCTGTGTTTGAGGACCTCGGTGTAAAGCACAAAG TGTTAAAGGAAGTGGAAAGCGTGACTCCAGAACACTGTATCTTTGCCAGCAACACATCTGCTCTCCCAAT CAATCAAATTGCTGCTGTCAGCAAAAGGCCTGAGAAGGTGATCGGCATGCACTACTTCTCCCCCGTGGAC AAGATGCAGCTCCTAGAAATCATCACCACTGACAAAACCTCCAAGGACACAACGGCCTCTGCCGTGGCCG TGGGGCTCAGGCAAGGGAAGGTCATCATTGTGGTCAAGGACGGACCCGGCTTCTACACCACCAGGTGTCT TGCACCCATGATGTCTGAAGTCATGCGAATCCTCCAGGAAGGAGTTGACCCTAAGAAGCTGGACGCCTTG ACCACAGGTTTCGGCTTCCCTGTGGGTGCTGCCACGCTGGCAGACGAAGTGGGTGTGGATGTAGCACAGC ACGTAGCAGAAGATCTAGGCAAAGCCTTTGGGGAGCGATTTGGAGGTGGCAGCGTAGAACTGCTGAAACA GATGGTCTCCAAGGGCTTCTTAGGTCGCAAGTCTGGGAAGGGCTTTTACATCTATCAGGAGGGCTCAAAG AATAAGAGTTTGAATTCTGAAATGGATAATATCTTGGCAAATCTGAGGCTGCCTGCCAAGCCCGAGGTCT CCTCTGATGAAGACGTCCAATACCGTGTGATAACAAGGTTCGTGAACGAGGCAGTCCTGTGCCTACAGGA AGGGATCCTGGCCACACCGGCAGAGGGAGACATCGGAGCTGTCTTTGGGCTTGGCTTTCCCCCTTGTCTC GGAGGGCCCTTCCGCTTTGTGGATCTGTATGGTGCTCAGAAGGTAGTGGACCGGCTACGGAAGTACGAGT CTGCCTATGGAACACAGTTTACCCCATGCCAGCTGCTCCTCGACCACGCTAACAACTCTAGCAAGAAGTT CTACCAGTGACAGGCCCTCCCACCCTACCCCACCATGCACTAACCCAGACCTGGCAGTGCCGCTTCTCAG CTGTGCTGTCCAAATTATCAGGAAGCAGGAAAAAGACCAAGGCTAGCCTTGGGCTTGCTCCTCCATCAGA GTGCCTTCTGCCCTGGCCCGCTCTTCCTCCTGGTGAAGTCTGACTGTGAATTAACTGTTTGCACTTCATG TTAAGGGGGAGCCCCACTGTGCTTCTTTTGCAAGCCCTGCCTGAGACCCAAATCAACAGCTAAGAGTAAC CCAGAACACCTGCTGCCTGTGCCTTCCCAGAGGCCAGTGGGGCCTGGCAGTGGTAAGGGCATTTTTTGCA CCAAGCCAAACACAGGATAACAATAAAATCCGGACTTTGTGCCTCAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6468514 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6468514 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14689 same embryo
	EMAGE:14687 same embryo
	EurExpress:euxassay_005619 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source