EMAGE mouse gene expression spatial database

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EMAGE:14721

Aco1 aconitase 1 ( MGI:87879)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005669. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005669_01	euxassay_005669_02	euxassay_005669_03	euxassay_005669_04

euxassay_005669_05	euxassay_005669_06	euxassay_005669_07	euxassay_005669_08	euxassay_005669_09

euxassay_005669_10	euxassay_005669_11	euxassay_005669_12	euxassay_005669_13	euxassay_005669_14

euxassay_005669_15	euxassay_005669_16	euxassay_005669_17	euxassay_005669_18	euxassay_005669_19

euxassay_005669_20	euxassay_005669_21	euxassay_005669_22	euxassay_005669_23	euxassay_005669_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14721_wholemount_strong.wlz

14721_wholemount_moderate.wlz

14721_wholemount_weak.wlz

14721_wholemount_possible.wlz

14721_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14721_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

latissimus dorsi

moderate

regional

moderate expression: see section 04 05 06 07 08 19 20 21 22

subscapularis

moderate

regional

moderate expression: see section 07 21 22 23

trapezius

moderate

regional

moderate expression: see section 05 06 07 08 09 21 22 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6124
Entity Detected:	Aco1, aconitase 1 ( MGI:87879)
Sequence:	sense strand is shown >T6124 CACCCGCCTGTGTGCTCCGGGAACTGCACTGTGAGCGTTTTTCAGAGACACGTGACCATCAATAAGCATG AAGAACCCATTTGCACACCTTGCTGAGCCCTTGGACGCTGCCCAACCAGGAAAGAGGTTCTTCAACTTGA ATAAATTGGAGGACTCAAGATACGGACGCTTACCATTTTCTATCAGAGTTCTCCTGGAGGCAGCCGTTCG GAACTGTGATGAGTTTCTGGTGAAGAAAAATGACATCGAGAATATCCTGAATTGGAATGTCATGCAACAT AAGAACATAGAAGTGCCGTTTAAGCCAGCCCGAGTCATACTGCAAGACTTTACGGGTGTGCCTGCTGTGG TGGATTTTGCAGCAATGCGCGATGCTGTGAAGAAGTTGGGAGGGAATCCGGAGAAAATTAACCCTGTCTG CCCCGCTGACCTTGTAATTGATCATTCCATCCAGGTTGATTTCAACAGAAGGGCAGACAGTTTACAGAAG AATCAAGACCTGGAGTTTGAAAGAAATAAAGAACGATTTGAATTTCTAAAGTGGGGTTCCCAGGCCTTTT GCAACATGCGGATAATCCCTCCTGGCTCGGGAATCATTCACCAAGTGAACCTGGAGTATTTGGCAAGAGT AGTGTTTGATCAGGATGGATGCTACTACCCAGACAGCCTCGTGGGCACAGATTCACACACGACCATGATT GACGGCCTGGGAGTTCTTGGTTGGGGTGTGGGTGGTATTGAAGCAGAAGCTGTCATGCTGGGTCAGCCCA TCAGCATGGTGCTTCCCCAGGTGATTGGCTACAAGCTGATGGGGAAGCCTCACCCTCTGGTAACATCCAC GGACATCGTGCTCACCATTACCAAGCACCTCCGACAAGTGGGAGTTGTGGGCAAATTTGTGGAGTTTTTC GGGCCAGGAGTGGCTCAGCTGTCCATTGCTGACCGAGCTACGATTGCCAATATGTGCCCAGAGTATGGTG CGACAGCAGCCTTCTTCCCGGTTGATGAAGTTAGCATCGCGTACCTGCTGCAGACAGGCCGCGAGGAAGA CAAAGTCAAGCACATTCAGAAGTATCTTCAGGCTGTAGGCATGTTTCGAGATTTCAACGACACCTCTCAA GACCCAGACTTCACTCAGGTTGTGGAGTTAGATCTGAAAACAGTTGTGCCTTGCTGCAGTGGACCCAAAA GACCTCAGGACAAAGTCGCAGTGTCTGAGATGAAAAAGGACTTTGAAAGCTGCCTTGGAGCCAAGCAAGG ATTTAAAGGTTTCCAAGTTGCTTCAGACCGTCACAATGACCGCAAGACGTTCCTCTATAATAACAGTGAA TTCACTCTCGCTCACGGCTCTGTGGTAATCGCTGCCATCACTAGCTGCACAAACACCAGCAATCCATCCG TGATGTTAGGAGCAGGATTGTTAGCAAAGAAAGCTGTGGAGGCGGGGCTGAGTGTGAAGCCTTACATCAA AACCAGCCTGTCTCCTGGAAGTGGAGTGGTCACCTACTACCTTCGAGAGAGTGGAGTCATGCCTTACCTG TCCCAGTTAGGGTTTGATGTGGTGGGCTACGGCTGCATGACCTGCATCGGCAACAGTGGACCCCTTCCTG AACCTGTGGTCGAGGCGATCACCCAGGGAGACCTCGTAGCTGTTGGCGTTCTGTCTGGGAATAGGAATTT TGAAGGCCGAGTCCATCCTAACACACGAGCCAACTACTTAGCATCCCCCCCTCTGGTAATAGCATATGCG ATTGCAGGCACCGTCAGGATTGACTTCGAGAAAGAGCCTTTGGGAGTGAACGCACAGGGCCGGCAAGTGT TTCTGAAGGATATCTGGCCCACGCGAGATGAGATCCAGGCGGTGGAAAGGCAGTATGTCATCCCCGGGAT GTTTAAGGAGGTCTATCAGAAAATAGAGACTGTAAATAAAAGCTGGAATGCCTTAGCAGCCCCGTCAGAG AAGCTGTACGCGTGGAATCCCAAATCTACTTACATTAAGTCGCCGCCATTCTTTGAAAGCTTGACTTTAG ATCTCCAGCCACCCAAGTCCATAGTGGATGCCTATGTGCTACTGAATTTAGGAGATTCAGTAACAACGGA CCATATCTCTCCCGCCGGCAATATTGCAAGAAACAGCCCTGCGGCTCGCTACTTGACGAACAGAGGCCTA ACACCACGAGAGTTCAACTCCTATGGCTCCCGCCGGGGTAACGACGCCATCATGGCCCGGGGGACATTTG CCAACATTCGCTTGCTGAACAAGTTTCTGAACAAGCAGGCACCTCAGACTGTCCATCTCCCTTCAGGAGA AACCCTTGATGTATTCGACGCTGCTGAGCGGTACCAGCAGGCTGGACTTCCCCTGATTGTTCTGGCTGGG AAAGAATACGGTTCAGGCAGCTCCCGAGACTGGGCAGCCAAAGGCCCTTTCCTGCTGGGAATCAAAGCTG TCCTGGCAGAGAGCTATGAGCGCATTCACCGCAGCAACTTGGTTGGCATGGGGGTGATCCCCCTTGAGTA TCTCCCTGGTGAAACTGCAGACTCTCTGGGACTCACAGGTCGGGAAAGATACACTATCAACATCCCTGAA GACCTCAAGCCTCGCATGACGGTGCAGATCAAGCTGGACACTGGGAAGACCTTCCAGGCCGTGATGAGGT TCGACACTGATGTGGAGCTCACATACTTCCACAATGGAGGCATCCTGAACTACATGATCCGAAAGATGGC CCAGTAGGTGCTGGCCTCTCAGGAGACCCGCGCTTGGTGCTAGACCCAATGAGGAACCAGGCCCCCACTG GTGGAGGCCTGGCAGAGCAGCCACATCTACTTCTGATGAGGGTGCTGGCAGGATGAGCAAGTGGGCACTG CCATTCCTGGAGGCACAGAGCCAGGAGTCTCTAGTTTCGTGATTTGTTCATCTTTTTATCCCTTTCTGTA ATCTGGATTCTAGAATCACGGGAAGGTCCGTAGTACCAAAGAGAACTCGCTTCTCTTTAAAGTCACGGGC ATTGATTTTTTTCCCTCTGACTAATCTTGAGCTCAGCACTAGCCAGTATTCTCAGAAGTGGCTCCTACCC TTTCTGTTGTTCTGTGTTTGTCTCTGCTCGGTGACACCCTTCCCTGGAGAGCCCAGTCCTCCGTGTATTA CACCAGTGTTAACTGACATAGCATAAGACCTTCACACTTCAGATTCATAGCAGTGATGGGATCCCTTCCT TTGAAGTGAACAAGTCCTTGTAGATAGGTCACCCGTCAAAAGTGTTTGATTATCTACCTTTCAATCAACA TGAGATTGCCTCTTACCATTTTCAACGATTGTTGACAGGGGTCCTTTGTTTGAAAATAACTGGGGAGAGA TACGGGATTTCTAATTTGAATAAGATTAAATATATTTTCATGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3494686 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3494686 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14718 same embryo
	EMAGE:14716 same embryo
	EMAGE:14720 same embryo
	EMAGE:14719 same embryo
	EMAGE:14717 same embryo
	EurExpress:euxassay_005669 same experiment
	MGI:4822921 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source