EMAGE mouse gene expression spatial database

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EMAGE:14738

Spred3 sprouty-related, EVH1 domain containing 3 ( MGI:2142186)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005710. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005710_01	euxassay_005710_02	euxassay_005710_03	euxassay_005710_04

euxassay_005710_05	euxassay_005710_06	euxassay_005710_07	euxassay_005710_08	euxassay_005710_09

euxassay_005710_10	euxassay_005710_11	euxassay_005710_12	euxassay_005710_13	euxassay_005710_14

euxassay_005710_15	euxassay_005710_16	euxassay_005710_17	euxassay_005710_18	euxassay_005710_19

euxassay_005710_20	euxassay_005710_21	euxassay_005710_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14738_wholemount_strong.wlz

14738_wholemount_moderate.wlz

14738_wholemount_weak.wlz

14738_wholemount_possible.wlz

14738_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14738_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

weak

single cell

weak expression: see section 08 09 10 11 12 13 14 15 16 17 not examined expression: see section 18

telencephalon mantle layer

weak

single cell

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata basal plate mantle layer

weak

single cell

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

rest of cerebellum

weak

single cell

weak expression: see section 09

rest of cerebellum mantle layer

weak

single cell

weak expression: see section 02 03 04 05 06 07 08 10 11 12 13 14 15 16 17 18

metencephalon rest of alar plate mantle layer

weak

single cell

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16

pons mantle layer

weak

single cell

weak expression: see section 05 06 07 08 09 11 12 13 14 15 16 17 not examined expression: see section 03

midbrain mantle layer

weak

single cell

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 not examined expression: see section 18

glossopharyngeal ix ganglion

weak

single cell

weak expression: see section 04 05 14

trigeminal v ganglion

weak

single cell

weak expression: see section 03 04 05 06 07 08 15 16 17 18 19 20

vagus x ganglion

weak

single cell

weak expression: see section 05

vestibulocochlear viii ganglion

weak

single cell

weak expression: see section 04 05 06 14 15 16

spinal cord mantle layer

weak

single cell

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15

dorsal root ganglion

weak

single cell

weak expression: see section 07 08 09 11 13 14 15 16

extraembryonic component

weak

single cell

weak expression: see section 03

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9092
Entity Detected:	Spred3, sprouty-related, EVH1 domain containing 3 ( MGI:2142186)
Sequence:	sense strand is shown >T9092 GCTCCTTGTATCTTTTCGGTCTCCGGTTGCTGGTTTTCTCTGCCTCCATCTCCTTCTCGGCCTCTCTTTC TGGCCATTTCTGGTTCTCTCTCTGCTCCCTGCCCATCTCCTGGTTGCTGGGTGACTCAGTACATGGTGCG GGTCCGAGCCGTGGTGATGGCCCGAGATGACTCCAGTGGGGGCTGGCTGCCTGTGGGGGGCGGGGGCCTC AGCCAGGTGAGCGTATGTCGGGTCCGAGGGGCCAGGCCCGAGGGGGGGGCCCGCCAGGGGCACTACGTCA TCCACGGGGAGCGGCTTCGGGACCAGAAAACCACCTTGGAGTGTACCCTGAGGCCAGGTTTGGTTTACAA CAAAGTGAATCCCATCTTCCACCATTGGAGCTTGGGGGACTGCAAGTTTGGGCTGACGTTTCAGAGTCCT GCAGAAGCGGATGAGTTCCAGAAGAGCCTGCTGGCTGCGCTGGCTGCCCTGAGTCGAGGTTCGCTGACTC CTTCCTCTTCCTCTTCCTCCTCCTCCCCTTCCCAGGACACTGCAGAGACTCCCTGCCCTCTGACGTCCCA CGTGGACAGCGACTCCTCCTCCAGTCACAGCCGCCAGGAGACTCCTCCCACAGCTCCCATTGCCACAGTG GAGTCAGCAGCTGCCTTCCCACTGGCCACGCGCCCACAAAGGCGCCGCTCCTCTGCTCAGAGCTACCCTC CGCTTCTACCGTTCACTGGGATTCCTGAACCTTCAGAGTCTTTAGCTGGGGCAGGGAGCCAGGGCTGGGG CAGCCGCGGCTATGAGGACTACCGGCGATCTGGACCACCGCCTCCACCTCTTGCCCTGTCTACCTGCGTT GTGCGCTTTGCCAAGACTGGTGCATTGCGGGGTGCAGCCCTGGGTCCTCCAGTGTCACTCCCTGCCCCTC TCACAGAGGCTGCACCCCCAGCACCCCCTGCTCGTCCACCCCCAGGTCCAGGCCCCACCCCTGCTCCAGC CAAAGCCTCCCCAGAGGCTGAGGAGGCTGCACGCTGTGTGCACTGCCGAGCACTCTTCCGCCGCCGGGCA GATGGGCGTGGTGGTCGCTGCGCAGAGGCCCCTGACCCAGGTCGCCTGCTAGTGCGCCGGCTCAGCTGCT TGTGGTGTGCCGAGAGCTTGCTGTACCACTGCCTGTCAGACGCCGAGGGTGACTTCTCGGACCCGTGTGC TTGTGAGCCAGGCCACCCACGCCCTGCTGCGCGCTGGGCTGCTCTGGCTGCTCTGTCTCTGGCAGTGCCC TGTCTCTGTTGTTATGCGCCCCTGCGCGCCTGTCACTGGGTTGCAGCACGTTGTGGCTGTGCGGGCTGTG GGGGTCGCCACGAGGAGGCTGCACGGTGAGGATTGTTGAGCTGGTTTGGAAATCGGTCTGAAGACCCAAA AATTGAAGGTCCAGGACCCTGAACAGCATATGAACCTAAAACCCAAACTTAGGTATATCTGGAACTTGAA TGGATTTGAGAGATCCCAGATCTGGTACCTGGACCCCAAACTGAGGACTGAGATACCGAGGCCCTGCTTC ATCAGGGTGTCTGCCCACCCGAAGTCCTGAATTCTGGATTCTCCATACCTTTTCCCCACTGAGAAGACTT AGAATATTTTCAAACATTGCATCTCAAGGGGCCCAGGAGCTCACATCTTGGACCCTGACTCTGTCTAAAT GCCCAACCCAGACTTCAGGTCATACTTGACATTGTAAACACTCATCTTACGGCCCCCAAACTAGTCAAGA GTTACTCAAACTTGACACTATTATCAAATGACTTAGACCTTGAGACCAGAGGACCCAGAATCACCAGAAA ATGGTCCCTGAATTTTCACCTGAGACCTGGTATCTTCACATTTAGATGCTCTTATAGCCCCAGGATGGTT TGGAATCACCAGATACTGGCCTGAACTCTACCCGCTCCTGAGAGTCCACAGACATTGCCATCAGTTCATG TGCAAGTGACCCGCAAATTCCCTAGCACCCCAGATTTAAAATGTATTAACTCCTTAGATATCCTCACATA TGAGGTATTTTCAGACCAAAATTACTTGGGAGGCTCTTTTCAGAGCTCAGCAAGGTCCAGATATCAGATA CCAAGATGCTGGGGGGGGGGGGGGGAGCCAGGCTCCAAACACCTACTCCTTAAGCCAGCCATGAGAATTT GAGTCTTGTGGATGGGCTCTGAGATGCCTGGTATCCTTAAATGTCTGCCTGAAGGCCACCTGCACTTTGG GCCCAACACACTGGTTTGTAGAGATTAGCTAACCTTCCGGTTTCCCTTAGGCCTGAATTTTAAAATATTC TGGAAACATCGGCTTCCTTCTGCCTACACTCCCCTTCCACCACGTTGGGTGGAAGCCTGGAACTTAGAAG ATGCGGTGGTCATGGTAACCCAGGTCCTTCATTCCTTGACTCTCAGCCCGAGAGCAAATCCAAAGGGCTT TATGAGCCCTGGATCTAAGACCCAAGATGCCATTGGACCCTGGGTGCAGGAGTCAAGCCCTCACAATTTT GGGATTGGTTTTGGCCTGACACCTTGATCCCTTCAGACCAGAGACATTCAGCAACAAGATGCCACAGTCT TTCAGCACCGTTAGGCTCCAGGTGTTCTTGAATTCAAAGTACTTGTAGACTAATGAAGCCCCCATTTCCT CACTACCTGATTTCCCCGTCTCCCATGTCCTGCCATCCAGAGGCCCATAAAACTGAAATGCCCTAGCAAT GCCCAGACTCCCACCCCCACCCCCTTTATCCTAGGGCAGGGTACTTAAGCCAGTGTAAATTCAGATTCCT GGAATCCGGGATCACCTAGCAGTTTCCCCTTTCCCCTCTCTAGGGCCTGGCTTTGCTATGGAAGATTGTG TGCTGTCAGCTCAGCAGAGGCAGGCAGCTACAGGGATCAAGCAGAGACCGCAGAAGGCCTACCCTTTGCA CACCTGTAATTCCGGGGCCAGCAATTGATCCCCAGAAGACCCACCCCCACCCCAACCTTGCAGATTTCCC ACACTATAACCCCTAGTACATTCAGGTCCCTCCCCTGTGAACCTCTTCCATCTCAGTTGGGGGTTGCTCT GAAAAGCTCAGTATTGTTTTGTAGTACTTCCCCATGGAGAAGAGAGGAGCCACAGCCTCTTTTCCCTAAG TAGAGACACTAGGATCAGGTGTCCTGGATAGGAGAACTAAGATTTCAGAGCCAAAGTCTTGATGGTTCAT GGGCTTAGGACCTACATCTGGCCCTAAGTACCTGGGAATGTACACTTGAATCATTTTTCATTACCCAGAC TTGGGTGGCCTTGTGGGGGTTCAAGGGATGAATATTTGTGTCACTTCCTTTAAATTATTAACTATTTATT GTATCCGGAACCTGTGATTCAGCTCCCCCAAGCAGATCCTGGGATGGGTGAGAGAAAATGGGGAAAGAGG GTGTCCCTCCCATCCCCCTTCTGCCCAGCTCTCATCAATTGTCAGACCCACCAGAGCTGAGGGCAGAGGG ACTGGGGGCTGGGGCAGCCTGCGTCCCCATCCTCTCTTTGTGCCTCGGTCTCCCCCCCTTCCATGGTAAG CGGGGGAAGCTCCATTCTTAACCTACCTCGTTTTGGGAAGGGGTGGGGAGGGGGACCAGAGTGCTGTATG GTGCTGTGAGATCTTCGGATTAAGCAGTGGGGACTTGGGGAAGGTTCCCTAGTCCCCCATCTCCAGTACC CCCTCTCCCAGGTCCCCTTGCCCTGTTCCTTGTCGATACCCACCTCTGATCCATGATTACCTTTCATTGC CCCCATATCTGGGGTGCATGGAGGACTGAACCCTGGGAGCCAGGAAGACCTCAGATCCTAGAGCTCCCGA GCCCAATTTTCCAGCTGCTTGCCCCCCAGACCTTGGGGGAGGTCCGCCAACATTTCTCTTCCTGATCTTG ATTCCAAGCCAGTCTGTAACCTTGTTAGTGTCAGGGCAGGCTGATGTGGAGGGAGGATGGGGGCCAAAGA CCCAGCCTTGTCATTGGGGGAGGGAGGGGACTTGAGATCTGCTCCAAGAAATGTCTGTGACTGTGAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6821036 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6821036 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14737 same embryo
	EMAGE:14736 same embryo
	EMAGE:14739 same embryo
	EurExpress:euxassay_005710 same experiment
	MGI:4828426 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source