EMAGE mouse gene expression spatial database

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EMAGE:14745

4933427D14Rik RIKEN cDNA 4933427D14 gene ( MGI:1921727)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005757. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005757_01	euxassay_005757_02	euxassay_005757_03	euxassay_005757_04

euxassay_005757_05	euxassay_005757_06	euxassay_005757_07	euxassay_005757_08	euxassay_005757_09

euxassay_005757_10	euxassay_005757_11	euxassay_005757_12	euxassay_005757_13	euxassay_005757_14

euxassay_005757_15	euxassay_005757_16	euxassay_005757_17	euxassay_005757_18	euxassay_005757_19

euxassay_005757_20	euxassay_005757_21	euxassay_005757_22	euxassay_005757_23	euxassay_005757_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14745_wholemount_strong.wlz

14745_wholemount_moderate.wlz

14745_wholemount_weak.wlz

14745_wholemount_possible.wlz

14745_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14745_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8781
Entity Detected:	4933427D14Rik, RIKEN cDNA 4933427D14 gene ( MGI:1921727)
Sequence:	sense strand is shown >T8781 TTTTCCAGTGTATTTAAAACTTATAATTTCTCAGAAAATAGCTCAATGCAACCATGTTGCATTAAATTGT TTACTGTGTTCAAAGTACTGCTTGTGGGAAATAAAAGAGTAAGACAAGGCCCTCAATTCTCAAGGAGCCA TGTCTAGCTGGGAAGCTGAGACACAGACACTGAAATAGGAAGATTTGCCTGATAACTAGGCAGCCGCTCA TGTTCATCATCTGACCTGATGGGACCAAGCCAGCCAGCTTCTACATGTGTTCATCTAGCACCCAGTCTCC AACTGGATGCCATGAGTGACCCCAGGAACCTTCAGCCCCAGAACCAGCTGCAATTTAATAGGAATGTTCC TACAAATCCCAGCAACTTGGCAGTCCGGTACTCTTGCCCACATGGAATTAAAATTGAAAAACTGAAACAC TCATACAATGAATTGAATCATGGTAAAGACTTGGATTTTAAAGCTGGAAATGAATTAAGCAGTTCTGTCT CATTTTCTGTCATATCAGAAGAGAGGCTTAGCTATGCTGTCCACCTAGCCAAGAGAGATGTAAAACGAAG ACAATTTGAAGAACACGTGAAAGATCATCTCAGAAGTCTGCCCCATAGCTCTCACAAAGGTATGCACACC AAGGTGGAAAGGAAGGACTCCAAGAGTCAGGATGTCTGTCATTGCAGCCACCAGCCGTCCAGAGTAAGCC TCTCCAGCTCGGGCGCTAAGGTGTATGTGTACACATCTCAGCCAGGACGAGCTGATCTTACTGTGCCAAA TTCACCACCTACCCATGATCCAGGACTTCAGCCGCAACCCAGAATAGAGGAGAACAAAAACCTGTGGGAA CAGAAAGGCCTGCTAGAAGTTCAGCGACTCCAAAAGGAATTGAGTGGTTGTATCCGTAAGATAGAAGCAC TAACGAAAAAAGATAGACTAGAAGAGGCTTTGGATCCAGATGAAGAGCATCGAATCCGAGTCAGGAGACA GGAGCAAGCCGTGCGCTGTGCCCGCATGCTCTACGTTCTTCAGCAGCAGGTAAAAGAAATCCAGGAAGAA TTGGATAAATTGAGTCCACAAAAAATTAAACATACTAAGAAGTCATGGGCAGTGTCTAGGCTGGCAGCCG CTCATCGAGCAGCCATCCGGGCCTTGCAAGTGTTTGTCACTCAGTTTACTGACCGAGGGGAGCACCCAGT TCCTGCTCGGTGTAGGGAGCTGGGCAGCCTTATTCGTCAGCTGTCGCTGTGTTCCGCCAAGCTGGATGCT GATCCTTCTGTGCCAGACGTGGTCATAGACATCTTGCAGCAAATCGAGGCTTTGGAGTCTCTCCTGGAAA AGAAATTGTCACCTAAAAAGGCAAAGAAATGTTTCACAGAAATTCGGAGCAGATTTCCTGTTGGTAGCCA AAAGATCTTAGAGAGATGGCCAACTGTACTGCCCAAAAGTGAGAGGAGACCCTTGGTCACAAAGGAGACA TTCCCACAGGAAACCAGTCGACCTCCTGTGGCAAAGAGGCTTCTTGCAGCTATGTGTCAGCCTGATGGGG AGCTTCAGAGGCTGGAGAGTGAACCCGATGTGCTGGATGCACATCTTCTTCCAGAAGAAGCCCCTCGTAT AGAGGACAATGGTACAGACTTCAAAGACGGAACATTAACCCCAGCCAAACCACAGGCAGTGAGAAGGAAG GCTTTGACAGGGTCCATGCCGATTAGGAAGAAAGATACTGTGGAGTCAGCCAGACCACAGCAGGGACTCC ACATAGCTGAAAGAAATCGACCAAACCAACCTTACAGCAAAAGCAGGTTACAGCAGACAACAGTGTCATC CAGATTAAAAATGAACCGACAGCCTATGAAAGACCATAGGGCTCCTTGGATACCTCCAAACCCCACATCC CCACCAGCCTCTCCTAAATGTGCTGCGTGGATGAAGGTGAAGTACAGCCCCAGGGATGCTGCAAAGGAGC AGTCCCTACAGCAAGAGGATACCCACAAGGAAAGTCAGCTGAGAGGTGATGCTGAGCAAGAAGCAGCCAG GCTTTCTTGGCCAGATGCTGAGTCTTCCAAAAGACTGAAGGAACTAGAAGAATTGGAAGCCAAAGAAATG GAAAGAAAGCAAAAGCAGAGGCTCAATTGGCTTGAGGCTGAGACTTCTAGAAGAACCAAAGAGTTGGATG AACTGAAAGCTGAAGAGATGGATAGACTTCAAAAATTAAGTGTCTCTGCCACCCAGTTAGCTGACAAGGT GGAGGAGGCAGTTCTGGAGCGGTTGAAGCCCCTCTTGATCAAGGCCCAGAGAGTGAATTCTTCGGTGGAA GCAAATAGTCATTTGAAGGATCGTCCGTCACGGCACGCAGCAGCAGCAGCCCAGCCTGCCGAGCAGGCTT CAGATGTACGTTTTGAATCCAGGAATATTCCTCAGCTGCGTGACTGTTTGGAAGATACTGCTCATGAGCT CTGGGCTAGGACTCAGGATAAGATCTTGGGGTCAGAAACTTCAGCCAGATTGGGGGACAGCAAGGACAGC CCAGATCTAGAGACCATGATGCTCCGGATGGAAGAAATGGAAAAATACCAGGAGACAGTTCGCCAAAGAT ATAATAAAATTGTATACGCTGACCCTCACTTATGGATGCACGAAGAAAGAAATGACCAGAACACCCCAGC TGTAAGTGAAGGACCTTTGGCTTCCCACCCAATCAAGATCACCAAGACAGCAACTCAAAAGTGCCCTGCT GTGAACATCTTGTTAGAAAGGCCCTGCAATGCCAACTCACTGGATGAAAGTGTAACAACAGAGGAGGGAT CGGAGAAAAGGGAGGCTCCCCTGCCCCTCTCCAGAGAAGACCTGCATCAGAGAAAAGGGCAAACTCCCCT CTCAGTCCCACCCAGGATGCGGCACAGCATTGGTGCTTACTGTAGCCGGTTTGAGCAATTCCTACGGATC ATAGCTCATGAGGCTATAGGCTCCTTCAACCCATGGCTAATAGCTGAAAGCTTTTCAGATGAGCTGGTAG ATGAAGCACTGGGGGCCGTGGCTGCAGAACTGCAGGATGTGTGTGAAGACTATGCAGAAGCTGTGTTCAC CTCAGAGTTCCTAGAGGCCGCTGCATGAGAGCTGTCCAGGCAGGGCCCTCAGATCACTGCCGGAGAGTCA GCGCCACCCGCTGTCAGCCCGTGGGGGAATCTCTGTCCTCAGCTGCCCACTGAAGGATGCCAGGGAGCTC ACCCTCTCCTTGTCTTAGCAGTGCTTCTAGAAGTTATAAAAAGGTGGGTTACATTTGCTAGCGCCTGAGG AGCATGAGACAATTAGAAAGGTACAACATTTCAGAATGTATAATTTTCCTCTGACTCAGTTGGAAATCAA AATGTTTTGAAAGGTAATGGATAAAGATATTTCACATAGGGATTCCAGCCTCTGTTAAACCCATGGGGGT GGAGGGATCAGTGCTAATCCAAGTGCCGTTACTGTCTGGTCCCAGCACCTGGCTCCTGTTGGCAAGTGTG CTCATCACATTGTCACTGGTGGTGTCGCCTTCCTGGGCGGCAAGCTACATGGAATGCAGAGTGGTGGTGC CACTAAGCCTCTGCTCAGTGGCAGTGGAAGTGGGGAGCCCAGCGCACGGGCACACGGAACACACAGGCCC AAGCACACCTTGCTCCGTGGCTCTCACTTTCTAGGGTGTTTCACGTTCTCAGTTTCTCAGAGTCACTTAT TGCTGTTTGGACACATGCTAGTATAAAAAGCATTTCTCTGAATATAGAAGAATTTGAAGACAGGCCTTCC CCATGCCAGCTCACTCCCAGAGAGCAGAAGCGTCTGCACCATGGAGCATCCCATCTGGAGGAGGGAATCA AGCCGGTGACTTGAGGACTTTGGGCTCTTCTGAGGACCAACGATGCTGCGTGGCCTTTGAAGGCTCTTAG GTCCCGGTGGCAAGGTCCTCAGGACTCGGGATTGAGAAACAGAAAAGGAATGTGCGTACAAATAGGCAGT CACCCGAAAAGACTTTTCCTGGATCCGTTTGCCTCAGCTATTTGTGGGAGGCTTGGTGAAGGAAGGGTAA GGAACAAAGGGAGAGACACCCAAGGACGAATGTGAGTCTGTTACCACAGACGCCTCGACATGGTCTCCTG GGGTGGTTTCTAAGGTACCTTCCTGTTTAATACTACATAACCTCGGAAAGGACATCAAGGGACTCATAGG TCTTCAGTCTGGAGAGTTAAACATGTACACTTGAACCGGTAGAATCCCTTGTCATTGGTAGTCCTCATTT GTCATTTGCGGTTTTTACTGTGCTGATTAAAAAAAAATAAACTCAGTATTTTAAATTGTGAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6406803 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6406803 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14748 same embryo
	EMAGE:14750 same embryo
	EMAGE:14747 same embryo
	EMAGE:14749 same embryo
	EMAGE:14746 same embryo
	EurExpress:euxassay_005757 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source