EMAGE mouse gene expression spatial database

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EMAGE:14749

Zfp185 zinc finger protein 185 ( MGI:108095)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005775. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005775_01	euxassay_005775_02	euxassay_005775_03	euxassay_005775_04

euxassay_005775_05	euxassay_005775_06	euxassay_005775_07	euxassay_005775_08	euxassay_005775_09

euxassay_005775_10	euxassay_005775_11	euxassay_005775_12	euxassay_005775_13	euxassay_005775_14

euxassay_005775_15	euxassay_005775_16	euxassay_005775_17	euxassay_005775_18	euxassay_005775_19

euxassay_005775_20	euxassay_005775_21	euxassay_005775_22	euxassay_005775_23	euxassay_005775_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14749_wholemount_strong.wlz

14749_wholemount_moderate.wlz

14749_wholemount_weak.wlz

14749_wholemount_possible.wlz

14749_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14749_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trunk mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 21 24 moderate expression: see section 06 08 09 13 15 16 17 18 19 20 22 23 weak expression: see section 07 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7343
Entity Detected:	Zfp185, zinc finger protein 185 ( MGI:108095)
Sequence:	sense strand is shown >T7343 CCACGCGTCCGGTCAGAGACGAATCAAATGCCAGGTCTCCAAACGCAAGGGCTCCAGCTGGTTACATAAT CCGGGGAGTGTTCACCCGAACAATAGATAGCTCTTCTCACTCCCAGCATCGCCCCTCTAAGACCAACGGA GCTCCAAGAAGTGCTTCTGGGCAGCTGGGAGCAGCTAATTCTGGTCATCCTCTTCAGTCGTCTGGCTATA AGATGACTACGGAAGATTATAAGAAACTAGCACCGTACAACATCAGGCGCAGCTCCATATCAGGGACTGA GGAAGAGGAGGTGCCATTCACCCCAGATGAGCAGAAACGGAGGTCCCAGGCTGCACTTGGTGTTCTGAGG AAGACAGCCCCCCGGGAGCACTCTTACGTCCTCTCAGCAGCTAAGAAGACCACTAGCAGTCCGACCCAGG AGCTGCAGTCGCCATTCCTTGCAAAAAGGGTTGATGTGGTAGATGAGGACGTCCTTCCTGAGAAAAACCA GGAACCACCTGCTCTGGCAAGACCTGATCCTGGCCTAAGCAGCTCGACAACCGAGAAGATTGCCCATCGG CAGATTACACCCCCCACAGCAGAACTCCACCTGGTGGCCCCAGACCTGGAAGCCCTGAGCACTCCAGATT CTTGTGAGGAGAACAATGCAGCCCCCAAGATCATCAAGGAAATACCTGGCACCCTACAAGATGGACAAAG TGACCCAACTGTGGCTTCTCAGCAACTTGCAGATCTCAGCATCCTAGAGCCACTGGGTAGCCCCAGTGGA GCCGAGCAGCAAATCAAAGCCGAGGACTGTACTAACATGCTGATGAGCCCTTCAAGCTGCATGGTCACTG TTACCGTCTCTGACACCTCTGAACAATCTCAATTGTGTGTTCCTGGTGTCTCAAGCAAGGTGGACTCCAG CTCAACTATCAAAGGGATTCTCTTCGTGAAGGAGTACATGAATACCAGTGAAGTGTCTTCTGGGAAGCCA GTGTCTTCACACTGTGACAGTCCCAGCAGCATTGAAGACTCATTGGATCTGGCAAAGAAACCCCCACATG AAGGTACTCCTTCTGAGAGACCAACCGAAGGGGTCTGTACTTACTGTAGCCATGAGATCCAAGATTGTCC GAAGATTACCCTTGAACATCTTGGCATCTGCTGCCATGAGTATTGTTTTAAGTGCGGGATTTGCAATAAA CCGATGGGTGATCTCCTAGATCAGATCTTCATTCACCGTGACACCATCCACTGTGGGAAATGCTATGAGA AGCTCTTCTAGGCTGAACAGAAGCTGATGATCCCCAGCTGTATTTGGCTGTCCAGTTGACCCAAATTGTT GGCTCCTCGTCACTTACATCCTCCATGGTTCCACTGTACTTCTGCCTACCTTGTGCGGAAGGAGCATACA TCTAAGATTGTGTAGCCATGATGTTATGATTTTGTGTAGCCTTTTATACCTTAGGAATATCTCAGTTCCT TGCTTCCAGGATGAATGAGTCCACTTTGACCTCCCTGAGACATGTTTTCTAATGAGTGCAGATGACCTAG TATGTTAGACATGGGTACAAGTCTAGGTATCAGCGTATGCAGACTTTCTGAGGCTTCTGAGGGCCCAAGC CAAAAAACTGTTGACGATCCCTGGTAGGGAAATTACAGGCATTAAATACTAAGGGTTGGCAATAGGCTAC TGTTTAGCTTGTCTATTCACTTTGTGTGTATGTGTGTGTGTGTTTAAATTTCTCACATTTTTAAAAATAG TTAATTTTTTGATTGAATTACATAGCAGATTTAAATTACCTCCTTCCATTCTCCTTTGGTTTTTCCAGTA AGGTATGTCAGCACATGTTGGAAAGTTTACCCAGGTTTAAATTTGATAAGATACAATATTAGGAGGCTTC TACCAAAGGGATATCTGTGTCTGTTTGAATTTTGTTAGTTAGTTAAAATCTTTACCCTCAGTATTGCTGA ACTTTTATCCTCTGAACAGCTTTTAATGTGTGTAAATCAGGGTAAAGAAAACAGATACGGTTTCTTGAGA CTTCTGTTCCTACTGTGAAATATTTCCCTGAAGCGTAGTGTTAGAAATGGCTTTGAAAAGTGTGAAATTC ACCCCATCTCTGGGCTTCTTGGGTCCCTCCTTGAAACACTACGGTAATTTAATTGCTCCCTTTCCCTCTC CTCCAGAGTCTTTCACCCAGAGACGAATTTGGCTTATGTAACTTCTCAAAGAAAAGCTTGCTATCCAGTG AAACGTCAAGTAGGCTCTCTGCCCTGATCGTGCAGCATTTTGTGACCTGTTTTCTAGTCCTGAATCTGTC TCAAACTAACATCAACTTCTAGGTTCCCAGGTACTCAAAACACTACCAGTTGATGTCTGGCACAGCAGAG GCAGGTTCGTCCCTGCAGTTTGCTAATATGCTGCTCTAAGTCAACCTCACTCTGCTGCCTTTGGGCTCAG CATTAAATTGGAAGCTCCTTGAAAGGAAAAAAATAGTCCTCAAGACTTGGGAATGCAAAATTGAGGCTTC TTTGTTGGATTGACTTAGAAGATAGCTCTTCACTCTCTGCAGACTTTGAATGGGCTTGTTCTTCCTACCA GGTAACCAGAGAAGAACATTCTATTTATTTGACTATTTATCTGTTACCTGAACTCTGAAGGGAAGAAGGA GTACATTGTGCCACACCCCAATACTAATTATGAACTAGTTCTTTCTCAAGTTCAGCTCTAGGATGGGACT TAGCAGAGAGTGGGCAATAACATATGTGTGCCAAATGAATTAGTAAGAGAAAACTAAGTCAGTCCCTTAA TTATCTAGGCATTTCAGTGCAGGCCTCATAGACAAGAGTAAAAGGCTCTAAATCGGATGGCTTCCCCACT TAAAGGTGTACTCTGCCATTTAAGGAGTTTTAGAAATATAAAATATACTCAAAGACAGCTTGTAAGGCTA GTATTGATTTGAATCCAAAACAGCCCTTTTGTGTCTTAGACACAAAATTCTGCAGCTAACAAAGAGAGAT GGTTTTGCATTGATTTGCTTCTTTTTAATTGCTTTCCGTTTTTAATTATCCTTGCTTAATCCACATTAAT GTACACAAATTATAATAAATGTTAAGATGTTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4191053 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4191053 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14748 same embryo
	EMAGE:14750 same embryo
	EMAGE:14747 same embryo
	EMAGE:14745 same embryo
	EMAGE:14746 same embryo
	EurExpress:euxassay_005775 same experiment
	MGI:4829294 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source