EMAGE mouse gene expression spatial database

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EMAGE:15321

Slc16a10 solute carrier family 16 (monocarboxylic acid transporters), member 10 ( MGI:1919722)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010493. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010493_01	euxassay_010493_02	euxassay_010493_03	euxassay_010493_04

euxassay_010493_05	euxassay_010493_06	euxassay_010493_07	euxassay_010493_08	euxassay_010493_09

euxassay_010493_10	euxassay_010493_11	euxassay_010493_12	euxassay_010493_13	euxassay_010493_14

euxassay_010493_15	euxassay_010493_16	euxassay_010493_17	euxassay_010493_18	euxassay_010493_19

euxassay_010493_20	euxassay_010493_21	euxassay_010493_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 04 05 19

lateral ventricle choroid plexus

strong

regional

strong expression: see section 10 11 12 14 15 16 17 18 moderate expression: see section 07 08 weak expression: see section 09 13

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30483
Entity Detected:	Slc16a10, solute carrier family 16 (monocarboxylic acid transporters), member 10 ( MGI:1919722)
Sequence:	sense strand is shown >T30483 ATGGCCTTCAAGACAGCGTGGGTGGGCTCGCTGTCCATGGGCATGATCTTCTTCTGCTGCCCCATCGTGA GTGTCTTCACGGACATGTTCGGCTGCCGGAGAACAGCTGTTGTGGGGGCAGCTGTGGGATTCATTGGACT CATGTCCAGTTCTTTTGTAAGCTCCATCGAGCCTCTGTACCTCACCTATGGAATCATATTTGCCTGCGGC TGCTCCTTTGCCTACCAGCCGTCACTGGTCATTTTGGGACACTACTTCAAGAAGCGCCTTGGACTAGTCA ACGGCATCGTCACGGCCGGCAGCAGCGTCTTCACAATCCTGCTCCCTTTGCTGTTAGGAAATCTAATCAG CAGTGTGAAGCTCTTTAACACGCTGCGGATCCTCTGCATCTTCATGTTTGTTCTCTTTCTGGCTGGCTTT ACCTACCGACCTCTTGTTCCCAGCACCAAAGAGAAAGAGAGTGGGGGCAGCAGAAGCTCATTCTTCTCCA GGAGAAAGCTTAGTCCTCCAAAAAAAGTCTTTAACTTTGCCCTCTTCAAGGAGACAACCTATGCAGTGTG GGCTGCTGGGATA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:30046388), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58906. Forward Primer - name:058906_F_IRAV107_g05_Slc16a10, sequence:ATGGCCTTCAAGACAGCG; Reverse Primer - name:058906_R_SP6_IRAV107_g05_Slc16a10, sequence:GTATCCCAGCAGCCCACA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15322 same embryo
	EMAGE:15318 same embryo
	EMAGE:15320 same embryo
	EMAGE:15319 same embryo
	EMAGE:15317 same embryo
	EurExpress:euxassay_010493 same experiment
	MGI:4828107 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source