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EMAGE:15764

LOC432457 (LOC432457)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15764 EMAGE:15764 EMAGE:15764 EMAGE:15764 EMAGE:15764
"Pseudo-wholemount" of euxassay_013345. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013345_01 euxassay_013345_02 euxassay_013345_03 euxassay_013345_04
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euxassay_013345_10 euxassay_013345_11 euxassay_013345_12 euxassay_013345_13 euxassay_013345_14
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euxassay_013345_15 euxassay_013345_16 euxassay_013345_17 euxassay_013345_18 euxassay_013345_19
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euxassay_013345_20 euxassay_013345_21 euxassay_013345_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15764Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15764_wholemount_strong.wlz
15764_wholemount_moderate.wlz
15764_wholemount_weak.wlz
15764_wholemount_possible.wlz
15764_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15764_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39902
Entity Detected:LOC432457, (LOC432457)
Sequence:sense strand is shown

>T39902
AGGAGCGAACATTAGCAGAAAGTGGAATTCCCACTGGATCCTCCCAGGACAGAGGAGGGAAGAATGGGAT
TAGCCAGAAAACCGGAACTGCTCAGGACAGCAGTGCTGTGTGGTTTATAGCAAGCATTCTCCTGCTGGCT
GGAGAGACCTGTGTTTCTGAGTTCCAGTCCTTGAGCAAGTCATACCTGTATTTTAAAAAGAGATTTTATT
ACTAGAATTTTTCTACTGCCTCACGTGCACACTTGGAAGTTTCCCATCCGGCTCCTGGGAAATCAAGAAG
GAAAGCATTTCTACAACAGTCGTTCCCTTTGGCCTATGCTGACAGTTACAGAAAACCTGGTGTCAACTAG
TCTTTTTTCTAAATGGCACTTTCTTAATGGCCCAAGATTAGAAAACATAGAACAATATAGACTTCCTTAT
CGCCAGCCCCCAAGTCTGTCTGGTCGAGTCCCTGGAAGGAAGGCTTTGCTGAATGGATTGGAGTGGAAAG
CCAGAGTGTGTAATTTACATGCAAGTATGCCCAAGGTCTATAGGTTCACAATTGTTCCTCAAAAGAACAG
TCACCCAGTTAGAGTTGTATGCCTTCCTCCTCCTTCTTCCATGCTGTGGGTTTGGTTCGTTTGGTTTGTT
TGTCTGTCAGGAGTTACATTGGACAGTTGGTCCTAAGAATAACAGTATTAGGAAGAACTTTGAAACCCCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 109567. Forward Primer - name:109567_F_cDNA_LOC432457, sequence:AGGAGCGAACATTAGCAGAAAG; Reverse Primer - name:109567_N_SP6_cDNA_LOC432457, sequence:GGGGGTTTCAAAGTTCTTCCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15763 same embryo
 EMAGE:15765 same embryo
 EMAGE:15766 same embryo
 EurExpress:euxassay_013345 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS