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EMAGE:15824

LOC432641 (LOC432641)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15824 EMAGE:15824 EMAGE:15824 EMAGE:15824 EMAGE:15824
"Pseudo-wholemount" of euxassay_013247. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013247_01 euxassay_013247_02 euxassay_013247_03 euxassay_013247_04
EMAGE:15824 EMAGE:15824 EMAGE:15824 EMAGE:15824 EMAGE:15824
euxassay_013247_05 euxassay_013247_06 euxassay_013247_07 euxassay_013247_08 euxassay_013247_09
EMAGE:15824 EMAGE:15824 EMAGE:15824 EMAGE:15824 EMAGE:15824
euxassay_013247_10 euxassay_013247_11 euxassay_013247_12 euxassay_013247_13 euxassay_013247_14
EMAGE:15824 EMAGE:15824 EMAGE:15824 EMAGE:15824 EMAGE:15824
euxassay_013247_15 euxassay_013247_16 euxassay_013247_17 euxassay_013247_18 euxassay_013247_19
EMAGE:15824 EMAGE:15824 EMAGE:15824 EMAGE:15824 EMAGE:15824
euxassay_013247_20 euxassay_013247_21 euxassay_013247_22 euxassay_013247_23 euxassay_013247_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15824Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15824_wholemount_strong.wlz
15824_wholemount_moderate.wlz
15824_wholemount_weak.wlz
15824_wholemount_possible.wlz
15824_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15824_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39920
Entity Detected:LOC432641, (LOC432641)
Sequence:sense strand is shown

>T39920
AGAGAAAGGTGCTCGAGTTTTGGAGGCCCTCGGGTAGCCCTCAGGGCTCCCGTCCACACTATCTTATTAT
TTATTATCCTATCTGGTCACCTCATTAGAGCCCCCAGGATAGCCTGGGTTGCAAAGAAATTTAGTGCTCT
TTCTCATAGGGTTAGGGTAGGGCATTGTGGCCCCGCTCTTCTAGCCACTTTCTGCCTGTGATGTCGACTG
TAGAAGAAATGATACCTTTCAAGGACTACTGATGCCAGAGAGAGCCGGCTCCAGCATGAGGTGCCCTTGG
GGACCTAGGGGTTCTGCAGTTCAGTTACAAAGAGGAACAAAGATAGGCAAGGCTAGCCAATTCCTTCCAG
GTGGTAGAAGGCCTGGGAAGGCAGTAGAGCTTTGAGAAGAGAGGGGGCCTTGAGGGGCTCATATAGGAGA
TGATCCTTTGCGACCTAGAAAAAGGGCCAGGCCTGCACAGAGGTGCTGTGTGTGTGTGTGTGTGTGGGGG
GGTGGGGCAGCTGAGGACAAGGGTTGGGTGGTGCTCTGGCAATGTTGAGACCATGCAGCTACGGGCAATG
GAGAGTGACCGATCTTGATGGCCTCACTCCGTTCTCTTATTCCAGGGGTGTGGGGTGGAGGGGCCCAATT
AGCCATTGGACTCTGAGCTGTGTTGAGGTGTGAGCCCTGCTCTCTTCTTCTAGAAGATGATTTCCATGAG
GTGTCACCTGCCATTGTGACACCTTAGTGGTCTCAATACCTTAGCCTCTGAGGGATACTCAAGTGTCGTG
GATGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 110082. Forward Primer - name:110082_F_cDNA_LOC432641, sequence:AGAGAAAGGTGCTCGAGTTTTG; Reverse Primer - name:110082_N_SP6_cDNA_LOC432641, sequence:GCCATCCACGACACTTGAGTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15822 same embryo
 EMAGE:15821 same embryo
 EMAGE:15823 same embryo
 EMAGE:15825 same embryo
 EMAGE:15826 same embryo
 EurExpress:euxassay_013247 same experiment
  Allen Brain Atlas same gene
  BioGPS same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS