EMAGE mouse gene expression spatial database

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EMAGE:15840

LOC432938 (LOC432938)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013312. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013312_01	euxassay_013312_02	euxassay_013312_03	euxassay_013312_04

euxassay_013312_05	euxassay_013312_06	euxassay_013312_07	euxassay_013312_08	euxassay_013312_09

euxassay_013312_10	euxassay_013312_11	euxassay_013312_12	euxassay_013312_13	euxassay_013312_14

euxassay_013312_15	euxassay_013312_16	euxassay_013312_17	euxassay_013312_18	euxassay_013312_19

euxassay_013312_20	euxassay_013312_21	euxassay_013312_22	euxassay_013312_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15840_wholemount_strong.wlz

15840_wholemount_moderate.wlz

15840_wholemount_weak.wlz

15840_wholemount_possible.wlz

15840_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15840_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 08 14 15

pons mantle layer

strong

regional

strong expression: see section 14 moderate expression: see section 09

facial vii ganglion

strong

regional

strong expression: see section 04 05 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 16

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 21

vagus x ganglion

strong

regional

strong expression: see section 07 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 17

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39938
Entity Detected:	LOC432938, (LOC432938)
Sequence:	sense strand is shown >T39938 GAAACTGGGAAAGATGGAGATGAGAATTTGCCTATAGGGGAAGAAGTAGAAGCAATACCAAAGGGGATGG TTTGAGGAGGGGTTGAAAGGCCATAGAGAAATGTATTTTATGTTAACATAAAAATTGCATGCACTATATG TAAAGGTATGTACCTACATATATAATTAGCTAAATGAAGATATGCCACTTGAGGCAATAGCCGTAGACCA TATGGCAAAATCTCCAGGACTTCCTTCAACTTGTTGGTTAAGGGAACCTAAGAGGCTCTGAAAATAATAT AGGTGATTGCCAAAGCACTTGGTTAAATCCCAGAACTTTTGGGTAAAGTCCTATTGTAGAAAACACCATG TAGTTCAGACACAGGATTGGGTGGAGCCAAGCTAAGCTTGAACTGACCAGGAATCCCCTCCTTGAGGTCT GCTCCTATCGTGCAGGAAGAGTCTGTGAAAGCTGCGGGGAAGATAAACAACTAAAATCCTACCTAGCTGT GAAGTCTAAGAACCAAGCCATGGCCAGCTCAGCAGATATTGCCAAGGCAAGCTAACAGTCAACTGTGACT CCATTTTCTGGCATTCATAAGCTCTACACTCAGAAGCACATATCCCCTAATATACTGACATATACAAATA CATAAATTAAAACTAGTAAAATAATACCTGGAAAAGAATTTATGCCATCAGTATTATATCCCCTTTATTA TGTCCTTGAGTGTGAGATTATTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 110912. Forward Primer - name:110912_F_cDNA_LOC432938, sequence:GAAACTGGGAAAGATGGAGATG; Reverse Primer - name:110912_N_SP6_cDNA_LOC432938, sequence:CCAATAATCTCACACTCAAGGAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15837 same embryo
	EMAGE:15839 same embryo
	EMAGE:15841 same embryo
	EMAGE:15838 same embryo
	EMAGE:15836 same embryo
	EurExpress:euxassay_013312 same experiment
	MGI:4825096 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	BrainStars same gene
	ViBrism same gene
Data Source