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EMAGE:15849

Zfp568 zinc finger protein 568 ( MGI:2142347)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15849 EMAGE:15849 EMAGE:15849 EMAGE:15849 EMAGE:15849
"Pseudo-wholemount" of euxassay_013289. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013289_01 euxassay_013289_02 euxassay_013289_03 euxassay_013289_04
EMAGE:15849 EMAGE:15849 EMAGE:15849 EMAGE:15849 EMAGE:15849
euxassay_013289_05 euxassay_013289_06 euxassay_013289_07 euxassay_013289_08 euxassay_013289_09
EMAGE:15849 EMAGE:15849 EMAGE:15849 EMAGE:15849 EMAGE:15849
euxassay_013289_10 euxassay_013289_11 euxassay_013289_12 euxassay_013289_13 euxassay_013289_14
EMAGE:15849 EMAGE:15849 EMAGE:15849 EMAGE:15849 EMAGE:15849
euxassay_013289_15 euxassay_013289_16 euxassay_013289_17 euxassay_013289_18 euxassay_013289_19
EMAGE:15849 EMAGE:15849
euxassay_013289_20 euxassay_013289_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15849Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15849_wholemount_strong.wlz
15849_wholemount_moderate.wlz
15849_wholemount_weak.wlz
15849_wholemount_possible.wlz
15849_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15849_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 11 12 13 14 15 16 18 20 21 weak expression: see section 01 10 17 19
tongue muscle
weak weak
regionalweak expression: see section 11 12 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39602
Entity Detected:Zfp568, zinc finger protein 568 ( MGI:2142347)
Sequence:sense strand is shown

>T39602
AAGGAACTACACTCGTTCCCCTAACACTGAGCCACTTGACAGCTGAGACACCTTCTACCTCTTCAATGTG
GAAAAGGGCGGCAGCATCTAAAGTCACGTCGGTTAGTTGCCAGTCCACGGCCTTAGAGGACTTTGTGATT
ACAGCAGCGTGTAGTATCAGCAGCCCAGTAATTGAGCGGTGTGGTGCTTTGTGCAAAGGACTTTGGTTGA
AGTGCTAATGAGTGAGTGAGAGAGGACTCTGCAGTCACTGGAGACTAGCAGAGCCTGCTGTGAAGGTCCT
TGTCCTTCCTCTTGGTCTGCTCTCTCCTGGAGCTCATCTGCCCCGCCCTGCCCATCATCTCAATGAGGAG
GGAAAGCTGCTAATTACTAAACAGGCTCCGGCAGAGCAGTTGTGACAGAGGTCAGGCAGCATGTGGGCAT
GGGTGCAACTTGTGTGGAGGCCAGGCCAGCGGTCAGGCTAAGGGGTCCTGCCTCAGGAGCCTTTGGCTTC
AGTTTTCTGATGCAGGGTCTCACAGGAGCTGGGATTTACCAATTAGGCTCAGCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 108397. Forward Primer - name:108397_F_cDNA_LOC243905, sequence:AAGGAACTACACTCGTTCCCCT; Reverse Primer - name:108397_N_SP6_cDNA_LOC243905, sequence:AGCTGAGCCTAATTGGTAAATCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15853 same embryo
 EMAGE:15848 same embryo
 EMAGE:15852 same embryo
 EMAGE:15851 same embryo
 EMAGE:15850 same embryo
 EurExpress:euxassay_013289 same experiment
 MGI:4829320 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS