EMAGE mouse gene expression spatial database

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EMAGE:16449

Mir182 microRNA 182 ( MGI:2676846)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019104. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019104_01	euxassay_019104_02	euxassay_019104_03	euxassay_019104_04

euxassay_019104_05	euxassay_019104_06	euxassay_019104_07	euxassay_019104_08	euxassay_019104_09

euxassay_019104_10	euxassay_019104_11	euxassay_019104_12	euxassay_019104_13	euxassay_019104_14

euxassay_019104_15	euxassay_019104_16	euxassay_019104_17	euxassay_019104_18	euxassay_019104_19

euxassay_019104_20	euxassay_019104_21	euxassay_019104_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16449_wholemount_strong.wlz

16449_wholemount_moderate.wlz

16449_wholemount_weak.wlz

16449_wholemount_possible.wlz

16449_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16449_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

strong

regional

strong expression: see section 04 17 18

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 14 15 moderate expression: see section 05 06

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 07 15 17 18 19 20 moderate expression: see section 03 06 16

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 15 moderate expression: see section 05 06 07 14 16

dorsal root ganglion

strong

regional

strong expression: see section 07 10 12 13 14 15 moderate expression: see section 05 06 08 09

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 14 15 weak expression: see section 08 09 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70219
Entity Detected:	Mir182, microRNA 182 ( MGI:2676846)
Sequence:	sense strand is shown >T70219 TTTGGCAATGGTAGAACTCACA
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-182 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16451 same embryo
	EMAGE:16452 same embryo
	EMAGE:16450 same embryo
	EurExpress:euxassay_019104 same experiment
	MGI:4826226 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source