EMAGE mouse gene expression spatial database

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EMAGE:16745

Mir532 microRNA 532 ( MGI:3629950)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019296. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019296_01	euxassay_019296_02	euxassay_019296_03	euxassay_019296_04

euxassay_019296_05	euxassay_019296_06	euxassay_019296_07	euxassay_019296_08	euxassay_019296_09

euxassay_019296_10	euxassay_019296_11	euxassay_019296_12	euxassay_019296_13	euxassay_019296_14

euxassay_019296_15	euxassay_019296_16	euxassay_019296_17	euxassay_019296_18	euxassay_019296_19

euxassay_019296_20	euxassay_019296_21	euxassay_019296_22	euxassay_019296_23	euxassay_019296_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

16745_wholemount_strong.wlz

16745_wholemount_moderate.wlz

16745_wholemount_weak.wlz

16745_wholemount_possible.wlz

16745_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:16745_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

weak

regional

weak expression: see section 01 02 20 21 22 24

fibula

weak

regional

weak expression: see section 24

tibia

weak

regional

weak expression: see section 24

femur

moderate

regional

moderate expression: see section 03 17 18 21 22 weak expression: see section 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70081
Entity Detected:	Mir532, microRNA 532 ( MGI:3629950)
Sequence:	sense strand is shown >T70081 CATGCCTTGAGTGTAGGACCGT
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-532-5p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:16747 same embryo
	EMAGE:16744 same embryo
	EMAGE:16743 same embryo
	EMAGE:16746 same embryo
	EurExpress:euxassay_019296 same experiment
	MGI:4826329 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source